FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651366
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA99631.529554812501727No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAAC87951.3502393431649793RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA84051.2903651710405517No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC67771.0404288833006328RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA64860.9957535394847136RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA64780.9945253513385716RNA PCR Primer, Index 1 (100% over 28bp)
AGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGG49130.7542610452495218No Hit
AGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTG42940.6592299874417762No Hit
CCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCCAAGG42000.6447987767246064Illumina Small RNA Adapter 2 (100% over 21bp)
CAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGG40540.622384343057513No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA40530.6222308195392452No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC35940.5517635246543419RNA PCR Primer, Index 1 (100% over 27bp)
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG34280.5262786206218931No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCCA29370.4508985731524212No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG28700.4406124974284811RNA PCR Primer, Index 1 (100% over 29bp)
CCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCAAGGAACTC26370.4048415176720922RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC24730.379663660676179RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC24400.3745973845733428RNA PCR Primer, Index 1 (100% over 27bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCC23730.3643113088494026No Hit
GTCCCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCCA23470.3603196973744408No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA22750.3492660040591618RNA PCR Primer, Index 1 (100% over 28bp)
TTCAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTC22690.34834486294955525No Hit
GAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGT22180.3405151635178993No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA22090.3391334518534894RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGC19330.2967609608115867RNA PCR Primer, Index 48 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17580.2698943451147281No Hit
TCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCCAAGG17090.2623716927196077Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGG16740.256998369580236Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTCGGGTGCCAAGG16300.25024333477645444No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCAAG15330.23535155350448136No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC14340.2201527251959728RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC14190.21784987242195633No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC14070.21600759020274315No Hit
GTCGGTAGAGCATGGGTGGTTCAGTGGTAGAATTCTCGGGTGCCAAGGAA14060.2158540666844754RNA PCR Primer, Index 1 (95% over 23bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA13570.208331414289355RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA13520.20756379669801617No Hit
TCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCCA13300.20418627929612537No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG13030.20004114430289577Illumina Small RNA Adapter 2 (100% over 21bp)
TCAGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCG12350.18960154506068785No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG11990.18407469840304838No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC10810.1659589232474523RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATTCTCGGGTGCCAAGGAAC10510.16135321769941938RNA PCR Primer, Index 1 (95% over 24bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC10420.1599715060350095RNA PCR Primer, Index 18 (100% over 50bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA10290.15797570029752858RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC9790.1502995243841404RNA PCR Primer, Index 1 (100% over 26bp)
ATACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT9020.13847821347752262RNA PCR Primer, Index 3 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC8510.1306485140458667No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA8190.12573576146129828No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT7980.12251176757767522RNA PCR Primer, Index 1 (100% over 30bp)
AGTCCCATGCTCTACCGACTGAGCTAGCCGGGCTGGAATTCTCGGGTGCC7930.12174414998633641No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA7820.12005539128539101No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA7500.11514263870082257RNA PCR Primer, Index 1 (100% over 28bp)
AGAGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCGGGTG7490.11498911518255481No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA7370.11314683296334166No Hit
AGTTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCT7150.10976931556145085No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGTTCAGTGGTAGAATTCT7150.10976931556145085No Hit
CGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCCC7040.10808055686050545RNA PCR Primer, Index 46 (100% over 37bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC7040.10808055686050545RNA PCR Primer, Index 1 (100% over 26bp)
TTCTACAGCCCGGCTAGCTCAGTCGGTAGAGCATGGAATTCTCGGGTGCC6970.1070058922326311No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA6860.10531713353168573RNA PCR Primer, Index 1 (100% over 28bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA6520.10009733391058177RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)