FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16248232
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA8103674.987416477066551No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC6810074.191268317685272RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA6281783.86613140432756RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3641242.2410068984736307RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC2804251.7258800834453865No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2653511.6331069128013436No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTAGCTATCTCGTATGC2562351.5770023470861323RNA PCR Primer, Index 38 (100% over 50bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2449631.5076286453812329RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC2286181.4070330851996697RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2267921.395794939412485RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC2162821.3311109787206388RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC2036231.2532009636494603RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1964481.2090423130344272RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1794961.1047109617834112RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA1791171.1023784003084152No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1604470.9874735909728517No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1283040.7896489907332688No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC1210280.7448687340259543No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA1185350.7295255262234069No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA1083460.6668171650921775No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA1017370.6261419704002258No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA956270.5885378790750896No Hit
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC925510.5696065885814531RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC896080.5514938486845831RNA PCR Primer, Index 1 (100% over 26bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG870420.5357013612311788Illumina Small RNA Adapter 2 (100% over 21bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA853290.5251586757254574RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA850320.5233307845432045RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA830710.511261779127723RNA PCR Primer, Index 1 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC713880.4393585714433422No Hit
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG652290.40145290884571316RNA PCR Primer, Index 1 (100% over 29bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA639160.3933720296460563No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC631840.38886692410595813No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTA626980.3858758294440897RNA PCR Primer, Index 2 (97% over 36bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC588010.3618916815072557RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA553100.34040626697107723RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC535480.32956201019286285No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC532290.32759871966377635RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT498450.30677183831447014RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA469470.2889360516270324RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA455040.2800550853779045No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA443790.2731312551420979No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC443460.27292815612184756No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC440790.2712849004125495RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGC439830.270694066899094No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC386010.23757046305099536RNA PCR Primer, Index 1 (100% over 26bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA381990.23509634771340043No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA339150.208730402175449No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG332720.20477304853845024RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA332630.20471765789656376RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA328630.20225585159049922RNA PCR Primer, Index 1 (100% over 28bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC321350.1977753641134617RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG316190.19459963397863841No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC313380.19287021504862806No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC303420.1867403173465273No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTGGAATTCTCGGGTGCCAAG281510.17325577330505867No Hit
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT268420.16519951216846238RNA PCR Primer, Index 1 (100% over 25bp)
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG263540.16219610847506363No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG250800.154355255390248Illumina Small RNA Adapter 2 (100% over 21bp)
GGTCCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA243090.14961012373530858No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA241210.1484530747714582RNA PCR Primer, Index 1 (100% over 28bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTTGGAATTCTCGGGTGCC237880.14640362102165946No Hit
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA233550.14373871569534458RNA PCR Primer, Index 1 (100% over 28bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCTGGAATTCTCGGGTGCCAAG230370.14178157968202323No Hit
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC229160.14103688327443872RNA PCR Primer, Index 1 (100% over 26bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC226070.13913513790300386RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG223720.1376888266981909No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC221130.13609480711501412RNA PCR Primer, Index 1 (100% over 24bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA218630.13455617817372376No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA216600.133306811473396RNA PCR Primer, Index 1 (100% over 23bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG214720.13214976250954566RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC212670.13088808677768757RNA PCR Primer, Index 1 (100% over 26bp)
GCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTGGAATTCTCGGG210670.1296571836246553No Hit
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTTGGAATTCTCGGGTG205690.1265922347736049No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA202940.12489974293818551No Hit
GGGGATGTAGCTCAGTGGTAGAGCGCATGCTTGGAATTCTCGGGTGCCAA201480.12400118363647195No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA200130.12317032400817515RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA199040.12249948178977257RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG194080.11944684197025252RNA PCR Primer, Index 1 (100% over 29bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGCCTGGAATTCTCGGGTGCCAAG191320.11774819561906798No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG189670.11673270051781634RNA PCR Primer, Index 1 (100% over 29bp)
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG186720.11491711836709373No Hit
AAACCGTTACCATTACTGAGTTTAGTGGAATTCTCGGGTGCCAAGGAACT186420.11473248289413887RNA PCR Primer, Index 1 (100% over 25bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGTCTGGAATTCTCGGGTGCCA186060.11451092032659306No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCGCTGCTGGAATTCTCGGGTGCCAA185330.11406164067573629No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT183790.11311384524790144RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT181910.11195679628405109RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC181140.11148289857013366RNA PCR Primer, Index 1 (100% over 27bp)
GCGGATGTAGCGCAGCTGGTAGCGCATAACCTGGAATTCTCGGGTGCCAA179740.11062126636301106No Hit
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG172410.10611000630714777Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC172210.10598691599184452No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG169720.10445444156631935RNA PCR Primer, Index 1 (100% over 29bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA165600.10191878107107283No Hit
GCGCCCATAGCTCAATTGGATAGAGCGTCTGACTGGAATTCTCGGGTGCC164850.10145719238868574No Hit
TCCTCCATAGCTCAATGGCAGAGCATTCGACTGGAATTCTCGGGTGCCAA162930.10027552536177474No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)