FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17572375
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA9182595.225582768407799RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC9092065.174064405067613RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA5962183.3929278199446578No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG4570862.6011623357685005RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4508812.5658512295577576RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC4421832.5163530826083558RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA4054542.307337511292583RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC2811451.5999260202448444RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2506521.4263979684021084RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2196581.2500188506106886RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1852271.0540806237062434RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1757451.0001209284459271RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1724760.9815178653995263RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1545060.8792550807730883No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1457410.8293756535471158RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1446470.8231499726132636No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC1308490.7446289986413334RNA PCR Primer, Index 7 (100% over 50bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1244970.7084813521222942RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC1202210.6841477034265431RNA PCR Primer, Index 1 (100% over 26bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA1157140.6584994913891833No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC1140090.6487967619630244No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC1116560.6354064262798853RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC1058100.6021382994615128No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC1031560.5870350479090048No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC896610.5102383713072365RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC886610.5045476209106624No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA868430.4942018366896905No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTTGGAATTCTCGGGTGCC753890.4290199816473299No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT736630.41919774646284297RNA PCR Primer, Index 1 (100% over 30bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA695470.3957746178305437No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA668200.3802559414990859RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG654410.37240839670221015RNA PCR Primer, Index 1 (100% over 29bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA638600.3634113203252264No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA609440.3468170921688161RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC609350.34676587541524695No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA593640.33782570654222893No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA566250.32223874120601226RNA PCR Primer, Index 1 (100% over 28bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG533130.30339097589255865Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC488230.2778395066119406RNA PCR Primer, Index 1 (100% over 26bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC478800.27247312898797116RNA PCR Primer, Index 7 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG478140.27209753946179727No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG477500.27173333143641654RNA PCR Primer, Index 7 (100% over 36bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC426620.24277879341864714No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC425180.2419593253615405RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA421030.2395976639469622RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT406250.2311867348608256RNA PCR Primer, Index 1 (100% over 25bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA382250.21752893390904757RNA PCR Primer, Index 1 (100% over 28bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA380500.21653305258964708No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC371860.211616244247007RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA362890.20651164114127998RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG349200.19872100384836994RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA345360.19653575569608547RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTAGTGGAATTCTCGGGTGCCAAGGAACT342400.19485129357869954RNA PCR Primer, Index 1 (100% over 25bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG336850.19169292710860086RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGC329710.1876297313254469No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC316780.1802715910626765RNA PCR Primer, Index 7 (100% over 44bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG310580.17674332581680052RNA PCR Primer, Index 7 (100% over 45bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT309430.1760888895211945RNA PCR Primer, Index 1 (100% over 30bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA305080.17361341309868472No Hit
TCTATTTTGTTGGTTTTCGGAACTGAGGCCTGGAATTCTCGGGTGCCAAG290520.16532768052127275No Hit
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT287770.16376272416221482RNA PCR Primer, Index 1 (100% over 30bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC285500.1624709238221925No Hit
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG284770.1620554990432426RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC281970.16046208893220185No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCT276890.15757118773074213RNA PCR Primer, Index 7 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA274000.1559265608661322No Hit
CATTGCACTTGTCTCGGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG272010.15479410153721396RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC270200.15376407571543405RNA PCR Primer, Index 1 (100% over 26bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG270100.15370716821146826No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTTGGAATTCTCGGGTGCC266540.1516812610702879No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCTGGAATTCTCGGGTGCCAAG265430.15104958777626815No Hit
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA263440.1499171284473499RNA PCR Primer, Index 1 (100% over 28bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG254730.14496048485193377RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG254270.14469871033369136RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT253990.14453936932258732No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT253860.14446538956743182RNA PCR Primer, Index 7 (100% over 41bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA252710.1438109532718258RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT250180.14237119342149254RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG243910.13880309292284054RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG239680.13639590550508968Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC236850.13478542314285916No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT233750.1330212905199212RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTGGAATTCTCGGGTGCC228120.12981739804664993No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTTCTGGAATTCTCGGGTGCC226770.1290491467431124No Hit
GGCTCCTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCAA226630.12896947623756036No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTTTGGAATTCTCGGGTGCCA225690.12843454570028243No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGTGGAATTCTCGGGTGCCAAGGA220540.1255038092460467RNA PCR Primer, Index 1 (100% over 22bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA219400.12485506370083724RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC217170.12358602636240121RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG216170.12301695132274379RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA213500.1214975209668585No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG211040.12009759636930126No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC209950.11947730457607467RNA PCR Primer, Index 1 (100% over 26bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCA209230.11906757054752132RNA PCR Primer, Index 1 (100% over 28bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA208530.11866921801976112RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC208010.11837329899913927RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG205520.1169563021503923RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA204380.11630755660518284RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC204350.11629048435399313RNA PCR Primer, Index 1 (100% over 24bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG201780.11482796150207356RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA199560.1135646149140341RNA PCR Primer, Index 1 (100% over 28bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGTCTGGAATTCTCGGGTGCCA196290.11170373953435435No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC194500.11068509521336757RNA PCR Primer, Index 1 (100% over 27bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC193570.11015585542648618No Hit
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC193230.10996236991300265RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA189340.10774866800873531No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA189120.10762347150001068No Hit
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG187810.10687798319805947No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC185230.10540976959574332No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTGGAATTCTCGGGTGC178850.10177907084272898No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA175860.10007753647415332RNA PCR Primer, Index 7 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)