FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16392106
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC15021319.16374625688731No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA11564247.054761602932534No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA5770953.520566545872751RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC5546053.3833663593927468RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA3562262.1731557860838624No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2963771.808047117313663RNA PCR Primer, Index 1 (100% over 28bp)
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGGTGGAATTCTCGGGTGCCA2766951.6879771275271158No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC2638281.609482027507631No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC2538021.5483184405957355No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2482301.5143264690943312RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1906381.1629866229513157RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1738101.0603274527385316No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1584530.966642114198139RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC1558980.9510553433463643No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC1450870.8851028659770745RNA PCR Primer, Index 41 (100% over 50bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC1438500.8775565506958044No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1369960.8357437415302218RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG1310250.7993176715670335No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1203800.7343778767658042RNA PCR Primer, Index 1 (100% over 27bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA1034450.6310659533314389No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC937750.5720741434932156RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC921120.5619290163204167RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA845610.5158641604684596No Hit
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGTGGAATTCTCGGGTGCCA727210.44363427127667426No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA646270.3942568453376278No Hit
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC631360.38516100371727713RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA630200.38445334601911435RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG530150.3234178695525761RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA511060.3117720200198803RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA504180.30757487781008735RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT501140.3057203266011091No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA497220.3033289316211108RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC492240.3002908839169293RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC468310.28569239364362337RNA PCR Primer, Index 6 (97% over 36bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC462630.282227311121585No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT451890.2756753769161815RNA PCR Primer, Index 1 (100% over 30bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA430160.2624189960704256No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCTGGAATTCTCGGGTGCCA428590.26146121797894667No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA424290.2588380040978261RNA PCR Primer, Index 1 (100% over 28bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT395270.24113436064896113No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA393500.24005457260952312No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA382230.23317931204202805No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA369650.22550488631540083No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGAC358400.21864182674270166RNA PCR Primer, Index 41 (100% over 39bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGCCTGGAATTCTCGGGTGCCAAG334940.2043300598470996No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA332320.2027317295288354No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG329450.20098088677562237Illumina Small RNA Adapter 2 (100% over 21bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA306090.18673012485399984No Hit
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGTGGAATTCTCGGGTGCCAA291210.17765258472584305No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC281310.17161309230186775RNA PCR Primer, Index 1 (100% over 26bp)
CTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCCA272840.1664459710058No Hit
TCCATTGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA268350.16370684767411825No Hit
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG256690.1565936677081029No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG255190.1556785930984097RNA PCR Primer, Index 1 (100% over 29bp)
GCGTCTGTAGTCCAACGGTTAGGATAATTGCCTGGAATTCTCGGGTGCCA254840.15546507568948129No Hit
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA245970.15005393449749532No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA230890.1408543844213794RNA PCR Primer, Index 1 (100% over 28bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTC229160.13979899837153323RNA PCR Primer, Index 41 (100% over 44bp)
CTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCAA224860.13717578449041265No Hit
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCG221800.13530903228663846RNA PCR Primer, Index 41 (100% over 45bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC221330.13502230890893457RNA PCR Primer, Index 1 (100% over 27bp)
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG212080.12937934881582636No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC205750.12551773396292093RNA PCR Primer, Index 1 (100% over 26bp)
TCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTGGAATTCTCGGGTGCC198170.12089355693527117No Hit
AGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCTGGAATTCTCGGGTGCCA197160.12027740669807772No Hit
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC196390.1198076683984352No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA194510.11866077488761968RNA PCR Primer, Index 1 (100% over 28bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA191660.11692213312920255No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC187270.11424401477150038RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG184010.11225525261976708RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG183750.11209663968742027RNA PCR Primer, Index 1 (100% over 29bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCT182560.11137068049706365RNA PCR Primer, Index 41 (100% over 43bp)
TCCGATGTCGTCCAGCGGTTAGGATATCTGGCTGGAATTCTCGGGTGCCA173240.10568501692216972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)