FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15549692
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA6504554.183073208138142RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC5161443.319319765304676RNA PCR Primer, Index 1 (100% over 50bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC4563572.934829834571643No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA3721582.393346440559723No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3588152.307537666984015RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3324132.137746522567778RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC3036411.952713918706557RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA2006701.2905078762974855No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1990481.2800768015212134RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC1902091.2232332318865222RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1879611.2087763538981995RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG1710501.1000217882129113RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1487880.9568549653587993RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1179090.7582722538812987RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC1163750.7484071067131105No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG1147110.7377059301238892RNA PCR Primer, Index 1 (100% over 39bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC1131930.7279436788844436RNA PCR Primer, Index 1 (100% over 36bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1123100.7222651098169661RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1059280.6812224962397969RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1048620.6743670549873271RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1018810.655196257263488RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1005680.6467523601110555RNA PCR Primer, Index 1 (100% over 27bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC913630.5875550461063795No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC907440.5835742598631536RNA PCR Primer, Index 1 (100% over 44bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC876700.5638053795534985RNA PCR Primer, Index 1 (100% over 26bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG862930.5549498986860962RNA PCR Primer, Index 1 (100% over 45bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC851390.5475285298255426No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT821670.528415611061621RNA PCR Primer, Index 1 (100% over 43bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA763950.49129590476776RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATGTCGTATGC722890.46489023705421306RNA PCR Primer, Index 1 (98% over 50bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC655460.4215260340847909RNA PCR Primer, Index 1 (100% over 26bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT591880.3806377644007354RNA PCR Primer, Index 1 (100% over 41bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC591710.38052843747644644No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA555950.35753119740249517RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC516590.33221879893183737RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC515790.33170431928812477RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT496340.31919603295036325RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA491280.31594194920388136RNA PCR Primer, Index 1 (100% over 34bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC470570.3026233574272725RNA PCR Primer, Index 1 (100% over 42bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA459330.29539491843311105No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA458060.2945781819987174RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA393590.25311755371103173No Hit
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA392720.25255805709849427RNA PCR Primer, Index 1 (100% over 28bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA388340.24974128104916804No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC373520.24021054564939293RNA PCR Primer, Index 1 (100% over 38bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA355440.22858330570148913RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG345330.22208156920407168RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC325360.209238871097897RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG319950.2057597025072908No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA313730.2017596232774257No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG313190.20141234951791973RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG304440.19578522841481363RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG301830.1941067385772014RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG272330.17513530171530087RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT272090.1749809578221871RNA PCR Primer, Index 1 (100% over 35bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA270730.17410634242787576RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA267480.17201626887529348RNA PCR Primer, Index 1 (100% over 37bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG262680.16892939101301815RNA PCR Primer, Index 1 (100% over 29bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA260390.16745669303289093No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC257480.1655852733288865RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA253430.16298072013259168RNA PCR Primer, Index 1 (100% over 28bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG248540.1598359633103987RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT245570.15792595763311582RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT237060.1524531804231235RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT231470.148858253912682RNA PCR Primer, Index 1 (100% over 30bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTGGAATTCTCGGGTGCCAAG229360.14750131385239013No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATATCGTATGC225640.14510898350912674RNA PCR Primer, Index 1 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGCTCTCGTATGC224640.14446588395448604RNA PCR Primer, Index 1 (98% over 50bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA220810.1420028126602122RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA217640.13996418707200117RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG216140.13899953774004012RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAGCTCGTATGC210060.13508949244782467RNA PCR Primer, Index 1 (98% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA208710.13422130804905977No Hit
TCAGTGCATGACAGAACTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG206080.1325299562203547RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG204880.13175823675478587Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA204530.13153315191066162No Hit
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG198110.12740445276986836RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA191450.12312140973596132RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCTGGAATTCTCGGGTGCCA188910.12148793686717398No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG187660.12068406242387308RNA PCR Primer, Index 1 (100% over 29bp)
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC179970.11573862684868613No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG177200.1139572410823314Illumina Small RNA Adapter 2 (100% over 21bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA172630.11101827611762341No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG171550.11032372859861148RNA PCR Primer, Index 1 (100% over 29bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA169810.10920473537353666RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG169070.10872884170310254RNA PCR Primer, Index 1 (100% over 29bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC168860.108593790796628RNA PCR Primer, Index 1 (100% over 26bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT167590.10777705436223431RNA PCR Primer, Index 1 (100% over 46bp)
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG162740.10465802152222693No Hit
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG161810.10405993893641109Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA160360.10312744458218209No Hit
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC158130.10169333257533332RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph