FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11174403
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC5323764.764245570881952RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA5043674.513592359251764RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC3870623.463827105573336No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3550373.177234613786526RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3462313.098429508941104RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA2815752.5198214168577953No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA2721942.4358706232449285No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2215021.9822267015070067RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1917971.7163959452688435RNA PCR Primer, Index 5 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1767711.5819279114955849RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1654911.4809829214142356RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1412401.2639601417632782RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1225241.09647020963894RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC979930.8769417032838354RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC955930.8554640458197186No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA886250.7931072469822326RNA PCR Primer, Index 1 (100% over 28bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA860040.7696518552266283No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA738680.6610465006497439RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC714200.6391392900363447No Hit
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC650970.58255461164234RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC648140.580022037866363RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA622870.5574078543614366RNA PCR Primer, Index 5 (100% over 36bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA575040.5146046728402404RNA PCR Primer, Index 1 (100% over 28bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC513820.4598187482588555No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC503600.4506728457887191RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG501900.44915151171834417RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT501830.4490888685507404RNA PCR Primer, Index 1 (100% over 30bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA500040.4474869932648751No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC498590.4461893847930847No Hit
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA490510.438958573446832RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC482510.43179935429212635RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA451250.4038247054451142RNA PCR Primer, Index 1 (100% over 28bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA386890.3462287873455074RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG380380.34040297275836573No Hit
TCCTCGATAGCTCAATGGTAGAGCACTCGGCTGGAATTCTCGGGTGCCAA350480.31364539116765344No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC345020.30875922409456685RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA332630.2976713834287165No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG294980.2639783082818831Illumina Small RNA Adapter 2 (100% over 21bp)
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG294720.2637456336593552Illumina Small RNA Adapter 2 (100% over 21bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA268580.24035288507135458No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG258850.23164548477444388RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG255350.22851332639426017RNA PCR Primer, Index 5 (100% over 39bp)
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC251790.22532747387041616No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG228310.20431516565135513RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC222640.19924106907545755No Hit
GGGATCTTAGCTCAGCTGGGAGAGCATCTGCCTGGAATTCTCGGGTGCCA211910.18963876638420862No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA206430.18473470126323527RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA203570.18217528041542802No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG203460.1820768411520508RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA198230.177396501629662RNA PCR Primer, Index 1 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA195720.1751502966198731RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCTTAGCTCAGTCGGTAGAGCATGCGGTGGAATTCTCGGGTGCCAA192770.1725103345565754No Hit
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG191170.1710784907256343No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC188200.16842063061444984RNA PCR Primer, Index 1 (100% over 27bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA185240.16577171952720876RNA PCR Primer, Index 1 (100% over 28bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTTGGAATTCTCGGGTGCC179990.1610734819569332No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG179560.16068867392736774RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC179300.16045599930483984RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA174380.15605307952469585RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC173550.15531031053739514No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA172930.15475547105290546No Hit
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC169510.15169490486426881No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC160930.14401664232084704RNA PCR Primer, Index 5 (100% over 35bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC158440.1417883353589449RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC151170.13528239495210614RNA PCR Primer, Index 1 (100% over 26bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA150160.13437854353382458No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGGCCTGGAATTCTCGGGTGCC149710.1339758374563724No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCTGGAATTCTCGGGTGCCA148250.1326692799606386No Hit
GGGCCTGTAGCTCAGCTGGCTAGAGCGTACGACTGGAATTCTCGGGTGCC146880.13144326368039527No Hit
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA141470.1266018417270256RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG139090.12447197402850067RNA PCR Primer, Index 1 (100% over 29bp)
CGAGAATTCTACCACTGAACCACCAATGCTGGAATTCTCGGGTGCCAAGG138890.12429299354963304Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGCCCTCTCTGCCCTTCCGTTTGGAATTCTCGGGTGCCAAGGAACTCC137060.12265532216799412RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT136990.12259267900039045RNA PCR Primer, Index 1 (100% over 25bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGATGGAATTCTCGGGTGCCAA135960.1216709295342221No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAG132700.11875354772867956No Hit
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGGTGGAATTCTCGGGTGCCA132530.11860141432164208No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT129400.11580036982736348RNA PCR Primer, Index 1 (100% over 30bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA126460.11316935678800917No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT126100.11284719192604742No Hit
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC124450.11137060297538937RNA PCR Primer, Index 1 (100% over 27bp)
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTTGGAATTCTCGGGTGCCAA123150.11020722986274972No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA122180.10933917454024167No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGC121680.10889172334307255No Hit
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT120150.10752252267973511RNA PCR Primer, Index 1 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC116350.10412189358124993RNA PCR Primer, Index 1 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG115170.10306590875593086RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)