FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8660785
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA3427363.957331812301079No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2139632.470480447211194RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA1717211.9827417491601513RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1457271.6826072925260238RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC997501.1517431733959451RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGC974901.1256485410964479RNA PCR Primer, Index 19 (100% over 50bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA876591.0121368905936357RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA789520.9116032784557058RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG764430.882633618084273RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC706100.8152840648971196RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC701630.8101228699246085No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA700690.8090375179617091No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC664190.7668935321682734RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA615510.7106861560470558No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC566390.6539707428368213RNA PCR Primer, Index 1 (100% over 27bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA540050.6235577952806819No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA539360.6227611007547237RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA490290.5661034190318776RNA PCR Primer, Index 19 (100% over 39bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC466420.5385424069527185No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA437130.5047233016406711RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA427850.4940083375814086No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA417920.4825428641860986No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA406970.4698996684480679RNA PCR Primer, Index 1 (100% over 28bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG394320.45529360213883613Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG380550.4393943505121072No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG350370.40454762472454864RNA PCR Primer, Index 15 (97% over 36bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA348130.40196125408955424RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC320520.3700819267537527RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA319120.3684654451068812RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC315050.3637661020334762RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG300930.3474627299950293RNA PCR Primer, Index 19 (100% over 45bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT293800.3392302198934623RNA PCR Primer, Index 1 (100% over 30bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG282590.32628682042101265RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC282410.3260789870664149No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA270030.31178467078907973No Hit
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC269120.3107339577186133RNA PCR Primer, Index 19 (100% over 44bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA262340.3029055680287641No Hit
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC257620.2974557156193116No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC250810.28959268703702956RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA250720.2894887703597307No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA235750.2722039630356833No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA233340.26942130534356873RNA PCR Primer, Index 1 (100% over 28bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTGGAATTCTCGGGTGCCAAG231900.2677586385067866No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG228240.2635326936299654Illumina Small RNA Adapter 2 (100% over 21bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAAT227310.2624588879645436RNA PCR Primer, Index 19 (100% over 41bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCT224030.25867170239187326RNA PCR Primer, Index 19 (100% over 43bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA206260.2381539317740828No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGC204510.23613332971549345No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC197860.22845504189285384RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC191260.22083448555760246RNA PCR Primer, Index 1 (100% over 26bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGCCTGGAATTCTCGGGTGCCAAG174160.20109031687081483No Hit
GGTCCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA172510.19918517778700198No Hit
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC172010.1986078629131193RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA169420.19561737186640704No Hit
GGGGATGTAGCTCAGTGGTAGAGCGCATGCTTGGAATTCTCGGGTGCCAA166910.19271925119951597No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGT162740.18790444515133445RNA PCR Primer, Index 19 (100% over 46bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA155790.1798797684043652RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC151940.17543444387546858No Hit
TCTATTTTGTTGGTTTTCGGAACTGAGGCTGGAATTCTCGGGTGCCAAGG149170.1722361194741585Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG148640.1716241657078429No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT144940.16735203564111104No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA143620.1658279243740608No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA141670.16357639636591834No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA136680.15781479392456918RNA PCR Primer, Index 19 (100% over 38bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA136380.1574684050002396RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGTAGCGCAGTTGGTAGCGCATCACCTGGAATTCTCGGGTGCCAA135970.15699500680365577No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC133930.15463956211821447RNA PCR Primer, Index 19 (100% over 42bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC125400.14479057036977597RNA PCR Primer, Index 1 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA124470.14371676470435416No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG123160.14220419973478154RNA PCR Primer, Index 6 (100% over 34bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG122230.14113039406935976RNA PCR Primer, Index 1 (100% over 29bp)
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCA113220.13072718004199388RNA PCR Primer, Index 1 (100% over 28bp)
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT112710.13013831887063354RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG111980.12929543915476485No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT110950.12810617051456652RNA PCR Primer, Index 1 (100% over 30bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAA110810.12794452234987935RNA PCR Primer, Index 19 (100% over 40bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC110090.1271131889314883RNA PCR Primer, Index 1 (100% over 26bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGTGGAATTCTCGGGTGCCAAGGA109610.12655896665256094RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC106170.12258704032024811No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCGCTGCGTGGAATTCTCGGGTGCCA105140.12139777168004978No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG102910.11882294734253304RNA PCR Primer, Index 1 (100% over 29bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC102600.11846501212072577No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTTTGGAATTCTCGGGTGCCA101580.11728728977800512No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG100480.1160171970554632RNA PCR Primer, Index 1 (100% over 29bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC99290.11464318765562244No Hit
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG97650.11274959486928726Illumina Small RNA Adapter 2 (100% over 21bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCTGGAATTCTC97100.11211454850801632No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG96890.11187207626098558RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC95610.11039415018384592No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA94170.1087314833470638RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC93010.107392112839656No Hit
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG90830.1048750199895275RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT89620.10347791799473142RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG88750.10247339011417557RNA PCR Primer, Index 1 (100% over 29bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGATGGAATTCTCGGGTGCCAA87810.10138803815127613No Hit
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG87210.10069526030261691RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTTGGAATTCTCGGGTGCCAAG87070.10053361213792976No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)