FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20023410
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA14075147.029342155007563RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC13945946.964817680904502RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG5911832.952459146568941RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA5733052.8631736552365457RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC4517552.2561341949248406RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC4349312.1721125422692737RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC3703911.8497898210145025No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3441061.7185184741260355RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2856861.426759977446399RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA2791121.3939284067998408No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA2724321.3605674557929943No Hit
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA2330931.1641024181195911No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC2234901.116143553970078RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA2042631.0201209484298628RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1894680.9462324349349087RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1748880.8734176646235581RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC1702940.8504745195748377RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1593520.7958284827609283RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1446550.7224293963915237RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCGATAGCTCAATGGTAGAGCACTCGGCTGGAATTCTCGGGTGCCAA1441010.7196626348858661No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1431760.7150430421191994RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC1367170.6827857992220107RNA PCR Primer, Index 25 (100% over 50bp)
GGGCCTGTAGCTCAGCTGGCTAGAGCGTACGACTGGAATTCTCGGGTGCC1275400.6369544448223354No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1244200.6213726832742276RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC1156540.577593926309255RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT924630.461774492955995RNA PCR Primer, Index 1 (100% over 30bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC875980.4374779320804998No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA741710.3704214217258699No Hit
GCGCCCATAGCTCAATTGGATAGAGCGTCTGACTGGAATTCTCGGGTGCC688800.3439973511005368No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA631110.31518607469956417No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG626930.31309851818446505No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC617200.30823920600936605RNA PCR Primer, Index 1 (100% over 26bp)
GGGATCTTAGCTCAGCTGGGAGAGCATCTGCCTGGAATTCTCGGGTGCCA607960.3036246073970418No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA601580.3004383369266274RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC594700.29700235873909586No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA494920.24717068671120454No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC459550.22950636280233985RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC455440.22745376536763717RNA PCR Primer, Index 1 (100% over 26bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC425120.21231148940165537RNA PCR Primer, Index 1 (100% over 24bp)
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT422720.2111128923594932RNA PCR Primer, Index 1 (100% over 25bp)
GCGGATATGGCGGAATTGGCAGACGCGCCAGATGGAATTCTCGGGTGCCA409800.20466044494918695No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG407990.20375650301322304Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC404420.20197358991300682RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG401830.20068010393834018RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA397490.1985126409537636RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCGATAGCTCAATGGTAGAGCACACGGCTGGAATTCTCGGGTGCCAA374980.18727079952915113No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC363260.18141765063992596No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT362460.18101811829253858RNA PCR Primer, Index 8 (100% over 36bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA355390.17748725117250258RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCCTTAGCTCAGTCGGTAGAGCATGCGGTGGAATTCTCGGGTGCCAA350410.17500016231001614No Hit
TCCTCGATAGCTCAATGGTAGAGCACACGGTGGAATTCTCGGGTGCCAAG342370.17098486221877293No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG341270.17043550524111528RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG328130.1638731864352775RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA326390.16300420357970993RNA PCR Primer, Index 1 (100% over 28bp)
GCCCAGATAGCTCAGTTGGTAGAGCAGAGGACTGGAATTCTCGGGTGCCA324400.16201036686558382No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA318550.15908878657531358RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA312210.1559224927222686RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT309560.15459904182154788RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG308160.15389986021361995RNA PCR Primer, Index 1 (100% over 29bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTGGAATTCTCGGGTGCCAA297400.14852615014125967No Hit
TCCTCCATAGCTCAGTCGGTAGAGCACGCGGCTGGAATTCTCGGGTGCCA294050.146853108436575No Hit
GCGGGCGTAGCTCATCTGGTAGAGCGCCACCTGGAATTCTCGGGTGCCAA291320.14548970430111555No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG288790.14422618325250294RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTAGTGGAATTCTCGGGTGCCAAGGAACT288360.14401143461578222RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC288260.1439614930723588No Hit
TCCACAGTAGCTCAGTGGTAGAGCTATCGGCTGTGGAATTCTCGGGTGCC286420.14304256867336784No Hit
GGTGGGTGTAGCTCAGTTGGTTAGAGCACCGGATGGAATTCTCGGGTGCC284590.14212863842871917No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA284490.14207869688529576No Hit
GCTGCTATAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA278460.13906722181686337No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA268880.13428282195689945RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA268640.13416296225268323RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA267810.13374844744226883No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA257260.12847961461109772No Hit
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT254100.12690146183891754RNA PCR Primer, Index 1 (100% over 30bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGCCTGGAATTCTCGGGTGCCAAG251810.12575780049452118No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA250120.1249137884106653No Hit
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC248780.12424457172879144RNA PCR Primer, Index 1 (100% over 27bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA246800.12325572916900768RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG239630.11967492050554826RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC234820.1172727322668816RNA PCR Primer, Index 1 (100% over 26bp)
GCGGAAGTGTCGGAACTGGCAGACGAGCAAGACTTGGAATTCTCGGGTGC232340.11603418198998072No Hit
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGGTGGAATTCTCGGGTGCCA229640.11468576031754832No Hit
GCGGAAGTGTCGGAACTGGCAGACGAGCAAGACTGGAATTCTCGGGTGCC229190.11446102337214291No Hit
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC228550.114141397494233No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGCACTTGGTGGAATTCTCGGGTGCCA227390.1135620755905213No Hit
TATGCGAAACCGAAAGGTGAGGTATATGGGCTGGAATTCTCGGGTGCCAA226780.11325743217563843No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGTGGAATTCTCGGGTGCCAAGGA223890.1118141215707015RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT221460.11060054206551231RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG216320.10803354673354838RNA PCR Primer, Index 1 (100% over 29bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC211240.1054965163276385RNA PCR Primer, Index 1 (100% over 26bp)
GCAGGTATGGCGGAATTGGCAGACGCGTATGGTGGAATTCTCGGGTGCCA207470.10361372014057546No Hit
TCCTCGATAGCTCAATGGTAGAGCACTCGGCTTGGAATTCTCGGGTGCCA200930.1003475432006836No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)