FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10762128
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3711373.4485466071394058RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3198972.9724325895399124RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA2815082.6157280418891133No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2630442.4441634591225827RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC2597632.413676923374262No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC2292692.130331473478108RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA2055711.910133386259669No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG1897351.762987765988288RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1160811.0786063871383058RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1095241.0176797748549358RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGC1081321.0047455298803358RNA PCR Primer, Index 15 (100% over 50bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA969560.9008998963773708RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC963430.8952039968303667RNA PCR Primer, Index 1 (100% over 26bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA930200.8643272036905711No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA928100.8623759167331962RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC872200.8104345162964053RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC830080.7712972750370558RNA PCR Primer, Index 1 (100% over 27bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCA729710.6780350503171864RNA PCR Primer, Index 15 (100% over 39bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT676700.6287789924074495RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA614090.5706027655497129RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG603720.5609671247173421RNA PCR Primer, Index 15 (100% over 36bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA566770.5266337661101967No Hit
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG558840.5192653348854427RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA558530.5189772877631635RNA PCR Primer, Index 1 (100% over 28bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA523990.4868832632356723RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA499510.46413683241827264RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC457840.4254177240783607RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC443720.41229764225067755No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC437240.40627652821077764No Hit
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGGTGGAATTCTCGGGTGCCA414530.38517475354316544No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC406900.3780850775980364RNA PCR Primer, Index 1 (100% over 26bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA403220.3746656795013031No Hit
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCG392090.3643238586272157RNA PCR Primer, Index 15 (100% over 45bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC386330.3589717572584158RNA PCR Primer, Index 15 (100% over 44bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC381250.3542515011900992RNA PCR Primer, Index 1 (100% over 26bp)
TCCACAGTAGCTCAGTGGTAGAGCTATCGGCTGTGGAATTCTCGGGTGCC344720.32030839997442884No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC332870.3092975664292415RNA PCR Primer, Index 1 (100% over 26bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC329280.30596179491639575No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAAT325310.30227293338269157RNA PCR Primer, Index 15 (100% over 41bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA324060.3011114530509208No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG321640.29886282712861245RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA319790.29714383623759166RNA PCR Primer, Index 1 (100% over 28bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCT305490.2838565012421335RNA PCR Primer, Index 15 (100% over 43bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC300950.2796380046771419RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA284940.2647617645878213RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA281370.2614445767602838No Hit
GCGCCCATAGCTCAATTGGATAGAGCGTCTGACTGGAATTCTCGGGTGCC278250.2585455218521839No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA276520.2569380330730131RNA PCR Primer, Index 1 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA258350.24005475497039247No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT257040.23883752358269666RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG251560.23374559380821341RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG247350.2298337280508093RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG242240.22508559645453016RNA PCR Primer, Index 1 (100% over 29bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC241600.22449091852466352No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC234170.21758707943261782RNA PCR Primer, Index 1 (100% over 26bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG229980.21369379736052202Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG228480.21230002096239703No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGATGGAATTCTCGGGTGCCAA227890.2117518022458012No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGC225870.20987485002965955No Hit
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG224260.20837886336233874Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC219230.20370506650729298RNA PCR Primer, Index 1 (100% over 26bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTC217920.20248783511959714RNA PCR Primer, Index 15 (100% over 38bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA217310.20192103271769302No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC201280.18702620894306404No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATC200000.18583685308333073RNA PCR Primer, Index 15 (100% over 42bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA195230.1814046441372933RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA190880.17736269258273085No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA188860.1754857403665892RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCTGGAATTCTCGGGTGCCA187830.17452868057321005No Hit
TCCTCGATAGCTCAATGGTAGAGCACTCGGCTGGAATTCTCGGGTGCCAA187720.17442647030401423No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGT178650.16599876901668517RNA PCR Primer, Index 15 (100% over 46bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT171080.1589648441274811RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA168900.1569392224288728RNA PCR Primer, Index 1 (100% over 28bp)
CGAGAATTCTACCACTGAACCACCAATGCTGGAATTCTCGGGTGCCAAGG167910.15601933000611032Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG165350.1536406182866437RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA164890.15321319352455204RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGTAGCGCAGTTGGTAGCGCATCACCTGGAATTCTCGGGTGCCAA162700.15117827998328956No Hit
GCGGATGTAGCGCAGCTGGTAGCGCATAACCTGGAATTCTCGGGTGCCAA159100.1478332166277896No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG156180.14511999857277297RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT153740.14285278896515632RNA PCR Primer, Index 1 (100% over 30bp)
CTGGCCCTCTCTGCCCTTCCGTTTGGAATTCTCGGGTGCCAAGGAACTCC151110.14040903434711052RNA PCR Primer, Index 1 (100% over 27bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA149320.13874579451201471No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG148270.13777015103332724Illumina Small RNA Adapter 2 (100% over 21bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC145700.13538214747120644No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG145480.13517772693281477RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT142570.1324738007204523RNA PCR Primer, Index 1 (100% over 35bp)
TCCTCCATAGCTCAATGGCAGAGCATTCGACTGGAATTCTCGGGTGCCAA140900.1309220629972065No Hit
TCCACAGTAGCTCAGTGGTAGAGCTATCGGCTGGAATTCTCGGGTGCCAA140850.13087560378393567No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT140530.13057826481900234RNA PCR Primer, Index 15 (100% over 37bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTGGAATTCTCGGGTGCCAAG139810.12990925214790236No Hit
CGGGAAGTAGCTCAGCTTGGTAGAGCACTTGGTGGAATTCTCGGGTGCCA139320.1294539518578482No Hit
GGGGATGTAGCTCAGTGGTAGAGCGCATGCTTGGAATTCTCGGGTGCCAA137120.12740974647393155No Hit
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG136230.12658277247771071RNA PCR Primer, Index 1 (100% over 29bp)
TCCGTTGTAGTCTAGCTGGTCAGGATATTCGGTGGAATTCTCGGGTGCCA135010.1254491676739024No Hit
GGGCCTGTAGCTCAGCTGGCTAGAGCGTACGACTGGAATTCTCGGGTGCC134270.12476157131749409No Hit
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT133320.12387884626534826RNA PCR Primer, Index 1 (100% over 30bp)
GGCTCCTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCAA131600.1222806493288316No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA130260.1210355424131733RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT129140.11999485603590666RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA127510.11848028568327751RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG118740.11033133967557344RNA PCR Primer, Index 1 (100% over 29bp)
GGGATCTTAGCTCAGCTGGGAGAGCATCTGCCTGGAATTCTCGGGTGCCA118010.10965303516181929No Hit
GGCGAGAATTCTACCACTGAACCACCAATGCTGGAATTCTCGGGTGCCAA117600.10927206961299847No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC117250.10894685512010263RNA PCR Primer, Index 1 (100% over 26bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTGGAATTCTCGGGTGC115870.10766458083382766No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAA115150.10699556816272768RNA PCR Primer, Index 15 (100% over 40bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG114640.10652168418736518RNA PCR Primer, Index 1 (100% over 29bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA114540.10642876576082351No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT111280.10339962505556521RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC110150.10234964683564439RNA PCR Primer, Index 1 (100% over 26bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA108950.10123462571714442No Hit
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG108800.10109524807733192No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)