FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14377308
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA9236996.4247006463240535No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC4424703.0775580518967804RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA3938872.739643610611945RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3147842.1894502086204177RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC2482951.7269922853429862RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGC2179881.5161948259020395RNA PCR Primer, Index 48 (100% over 50bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1978361.3760295042715924RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA1843701.282368020494518No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1689451.1750808983155958RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1609631.1195628555776924RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG1571671.0931601381844223RNA PCR Primer, Index 1 (100% over 29bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG1215660.8455407646549689Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1085130.7547518631443383RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1022980.711524021047612No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA946760.6585099241109671No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC849520.590875565856974RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC797220.5544987976886911RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA771020.5362756365795321No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA708910.4930756161028198No Hit
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA698740.48600196921426464No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA678260.47175729976710523RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC675360.46974023231609147RNA PCR Primer, Index 1 (100% over 26bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA642800.4470934336247092No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG628070.43684812205455986RNA PCR Primer, Index 10 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA625490.435053627563658RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC622790.43317566821271414RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA619490.4308803845615604No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA566750.39419757857312376RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA565900.39360636914782654RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC560140.389600055865813No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC555450.38633797091917343RNA PCR Primer, Index 1 (100% over 26bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA492170.3423241680570521No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA491880.34212246131195073No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGC485830.33791444128483583No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCA483950.3366068251441786RNA PCR Primer, Index 48 (100% over 39bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA472740.32880981613525984No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC469050.3262432716889699RNA PCR Primer, Index 1 (100% over 26bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTGGAATTC463890.32265428270716606No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA458420.31884967617025384No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG438340.30488322292323433Illumina Small RNA Adapter 2 (100% over 21bp)
GGTCCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA429610.2988111543551825No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTGGAATTCTCGGGTGCCAAG404490.2813391769864011No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT401400.2791899568403209No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGTGGAATTCTCGGGTGCCAAG396510.2757887637936114No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG388540.27024530600582525No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA387990.26986275873063303RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCG364420.25346886913739347RNA PCR Primer, Index 48 (100% over 45bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTC362970.25246033541188656RNA PCR Primer, Index 48 (100% over 44bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA357590.24871832752000586RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG349460.24306358325216376RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT324480.2256889815534313RNA PCR Primer, Index 1 (100% over 30bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA319170.22199566149657501No Hit
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG308790.2147759511029464RNA PCR Primer, Index 1 (100% over 29bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC303020.21076268241592933No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCT302120.2101366959656147RNA PCR Primer, Index 48 (100% over 43bp)
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG280660.19521039682811275No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC277060.19270645102685427RNA PCR Primer, Index 1 (100% over 27bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCTGGAATTCTC273520.19024423765561677No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC273320.19010512955554684RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA272460.1895069647252462RNA PCR Primer, Index 1 (100% over 28bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTTGGAATT271790.189040952590012No Hit
GGGGATGTAGCTCAGTGGTAGAGCGCATGCTTGGAATTCTCGGGTGCCAA268980.18708648378402964No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG255370.1776201775742719RNA PCR Primer, Index 1 (100% over 29bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC253800.17652817898872306No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAAT250360.17413551966752053RNA PCR Primer, Index 48 (100% over 41bp)
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG247370.1720558535714753No Hit
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA240470.16725662411906317RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA239730.16674192414880448RNA PCR Primer, Index 1 (100% over 28bp)
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT236090.16421015672753203RNA PCR Primer, Index 1 (100% over 25bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA231630.1611080460959729No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG229790.15982825157532968No Hit
ACTGGACTTGGAGTCAGAAGGCTTGGAATTCTCGGGTGCCAAGGAACTCC222490.15475080592277776RNA PCR Primer, Index 1 (100% over 27bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTGGAATTCTCGGGTGC217640.1513774344960823No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCGCTGCTGGAATTCTCGGGTGCCAA210700.14655038342365623No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC209690.14584788751830316No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATGTCGTATGC208330.14490195243782772RNA PCR Primer, Index 48 (98% over 50bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA202170.14061742295567434No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG195510.13598512322334613RNA PCR Primer, Index 1 (100% over 29bp)
GCTGAGAAGACGGTCGAACTTGACTATCTAGAGGAAGTGGAATTCTCGGG195300.13583905971827273No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGGAATTCTCGGGTGC193020.1342532273774757No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATC192780.13408629765739177RNA PCR Primer, Index 48 (100% over 42bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA188700.13124849241596548No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC186920.13001043032534323No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG184120.12806291692436442No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGG179380.12476605495270743RNA PCR Primer, Index 48 (100% over 37bp)
GGCTCCATAGCTCAGGGGTTAGAGCACTGGTCTGGAATTCTCGGGTGCCA173660.12078756329070783No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTGGAATTCTCGGGTGCC172290.11983467280522891No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCGCTGCGTGGAATTCTCGGGTGCCA172140.11973034173017648No Hit
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC166570.1158561811432293RNA PCR Primer, Index 1 (100% over 26bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTTGGAATTCTCGGGTG166270.11564751899312445No Hit
TCTATTTTGTTGGTTTTCGGAACTGAGGTGGAATTCTCGGGTGCCAAGGA165060.11480591498770146RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC164540.11444423392751968RNA PCR Primer, Index 1 (100% over 26bp)
CCCTCGGATCGGCCCCGCCGGGGTCGGCTGGAATTCTCGGGTGCCAAGGA163120.11345656641702326RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT161460.11230196918644297RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC160530.11165511652111787RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA155230.10796875186926509RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT155090.10787137619921615RNA PCR Primer, Index 1 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC155080.10786442079421266RNA PCR Primer, Index 1 (100% over 24bp)
GGCTCCGTGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCAA151000.10502661555278638No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG150820.10490141826272345RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA150320.10455364801254866RNA PCR Primer, Index 1 (100% over 28bp)
TCTATTTTGTTGGTTTTCGGAACTGAGGCCTGGAATTCTCGGGTGCCAAG149560.10402503723228299No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGC147390.10251571434652439RNA PCR Primer, Index 48 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG146530.10191754951622375RNA PCR Primer, Index 1 (100% over 29bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT145750.10137502792595109RNA PCR Primer, Index 3 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)