FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15313820
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA8725685.697912082027868RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA6953074.540389008098567RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC6387484.171055948156632RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC4934233.2220765295661042RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG3404472.2231357035671047RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA3355182.190949090429429No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3115852.0346654198625815RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3094022.0204103221795737RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2328541.5205481062203943RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC2173251.419142970205997RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC2023591.3214142519632595RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1881951.2289226332815717RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1701631.111172783799209No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1555791.0159385443997644RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC1176490.7682537733890042RNA PCR Primer, Index 10 (100% over 50bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1157650.755951160455066RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC1138600.7435114164852401RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1052570.6873334021165195RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA1001380.6539060796065254No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT992290.6479702647673801RNA PCR Primer, Index 1 (100% over 30bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG956560.6246383985184625RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC937330.6120811136607326RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA919260.60028131452505RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA811920.5301877650383771RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC732820.47853507485395547RNA PCR Primer, Index 1 (100% over 26bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA652240.4259159373689909RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA610970.39896642379236535RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA576800.37665324523861454RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC571300.3730617181082186No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC570410.37248054371802725RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG531170.3468566301549842RNA PCR Primer, Index 7 (97% over 36bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG475820.310712807124545RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG453140.2959026552486577RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC430430.2810729132247865RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG429570.28051132898257913RNA PCR Primer, Index 1 (100% over 29bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG420830.27480406586991357Illumina Small RNA Adapter 2 (100% over 21bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG406760.26561628646542795RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG387850.2532679631861939RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT381780.2493042232441024RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA379790.24800474342783185RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA378560.24720154736048877RNA PCR Primer, Index 1 (100% over 28bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA373930.24417813452162818No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC360860.23564336004994182No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC359600.23482057383461477No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG359310.23463120240410298RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA357950.23374311569549594RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT355490.23213672356080978RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA353700.2309678447310991RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC347220.2267363727665599RNA PCR Primer, Index 1 (100% over 26bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG330250.2156548790569564Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA324630.21198499133462453No Hit
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG314420.20531781097074409RNA PCR Primer, Index 1 (100% over 29bp)
TTTGGTCCCCTTCAACCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC311690.20353510750420206RNA PCR Primer, Index 1 (100% over 27bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC303990.19850696952164776No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC302560.19757317246774483RNA PCR Primer, Index 1 (100% over 26bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA299810.19577740890254686No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC294900.1925711546825025RNA PCR Primer, Index 1 (100% over 26bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC290950.18999178519794538RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG281820.18402985016148812RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA277260.18105214766792346RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT277120.1809607269773316RNA PCR Primer, Index 1 (100% over 30bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA276160.1803338422418443No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG269090.1757170973669535RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA251830.16444623222683824RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA247320.16150117997991356No Hit
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACT243580.1590589415312443RNA PCR Primer, Index 1 (100% over 25bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA243180.15879773955812462RNA PCR Primer, Index 1 (100% over 28bp)
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG237130.1548470597146891RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG233530.15249624195661174No Hit
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG226460.14787949708172093RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA222690.14541766848506774No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA216170.14116007632321653No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT214970.14037647040385742RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC214080.13979529601366608No Hit
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG211390.13803871274443608RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCATGACAGAACTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG210320.13733999746634085RNA PCR Primer, Index 1 (100% over 29bp)
CTGGCCCTCTCTGCCCTTCCGTTTGGAATTCTCGGGTGCCAAGGAACTCC202780.13241634027303442RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC200740.13108421021012392RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT194960.1273098416985442RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA190280.12425377861304365RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC189540.1237705549627722No Hit
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC187930.12271921702096536No Hit
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT187670.12254943573843757RNA PCR Primer, Index 1 (100% over 25bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT184780.12066225148264771RNA PCR Primer, Index 3 (100% over 34bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA182650.11927135097578527RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG179550.11724703568410756RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT179220.11703154405628381RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT177960.11620875784095672RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC177550.11594102581850903RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC176800.11545127211890957RNA PCR Primer, Index 1 (100% over 27bp)
GGGGATGTAGCTCAGTGGTAGAGCGCATGCTTGGAATTCTCGGGTGCCAA175410.11454359526231861No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT174640.11404078146406318RNA PCR Primer, Index 10 (100% over 39bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG172350.11254540016795286RNA PCR Primer, Index 1 (100% over 29bp)
TCCTCGATAGCTCAATGGTAGAGCACTCGGCTGGAATTCTCGGGTGCCAA170100.11107613906915453No Hit
TTGGTCCCCTTCAACCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA169660.11078881689872286RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC168080.10975706910490002RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG162690.10623737251711199RNA PCR Primer, Index 1 (100% over 29bp)
AAGGTTACTTGTTAGTTCAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG161780.10564313802826465RNA PCR Primer, Index 1 (100% over 29bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT158950.10379513406844276RNA PCR Primer, Index 1 (100% over 30bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA154460.10086314192017407RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)