FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8320188
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA6255717.518712317558209RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC5007296.018241414737263RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2781923.343578294144315RNA PCR Primer, Index 1 (100% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2664713.2027040735137238RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC2278692.738748210977925RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2271922.7306113756083397RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA1951602.3456200749309994No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1800602.1641337912076026RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1362091.6370904118993466RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1346951.618893707690259RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1136291.3657023134573403RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1081161.29944179145952No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA939591.1292893862494453No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA783140.9412527697691446RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC742150.8919870560617139RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC650910.7823260724397093RNA PCR Primer, Index 11 (100% over 50bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA622000.7475792614301504RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC582910.700597149968246RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT580560.6977726945593056RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC567000.6814749859017609RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA558690.6714872308173806RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA403590.4850731738273222RNA PCR Primer, Index 1 (100% over 28bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC372690.44793458993955426No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC306650.36856138346873896RNA PCR Primer, Index 1 (100% over 27bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC303850.36519607489638456No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA302720.3638379325082558No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC247050.2969283867143387RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG225080.2705227333805438RNA PCR Primer, Index 1 (100% over 29bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA224800.2701862025233084No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG210640.2531673563145448Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA209570.251881327681538RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG204300.24554733618999952RNA PCR Primer, Index 1 (100% over 29bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC203070.24406900421000102No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA197580.23747059561634903No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG195310.23474229188090462No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA190240.2286486795731058No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA182710.2195984033053099RNA PCR Primer, Index 1 (100% over 28bp)
GCGGATGTAGCGCAGTTGGTAGCGCATCACCTGGAATTCTCGGGTGCCAA178670.21474274379377004No Hit
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT175510.2109447526906844RNA PCR Primer, Index 1 (100% over 30bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA164950.1982527317892336No Hit
AAACCGTTACCATTACTGAGTTTAGTGGAATTCTCGGGTGCCAAGGAACT163420.1964138310336257RNA PCR Primer, Index 1 (100% over 25bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC162640.19547635221704124RNA PCR Primer, Index 1 (100% over 24bp)
CATTGCACTTGTCTCGGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT159850.19212306260387385RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC157660.18949091054192524RNA PCR Primer, Index 6 (97% over 36bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA151320.18187089041738RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC148990.17907047292681366RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGAGATTGGAATTCTCGGGTGCCAAGGAACTC145540.1749239320073056RNA PCR Primer, Index 1 (100% over 26bp)
GGCTCCTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCAA141850.17048893606731003No Hit
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG139700.16790485984210934Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC138070.1659457694946316RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGTGCTGTTCGTGCAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG135350.16267661259577307RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA134940.1621838352691069RNA PCR Primer, Index 1 (100% over 28bp)
TCCGTTGTCGTCCAGCGGTTAGGATATCTGGTGGAATTCTCGGGTGCCAA131850.15846997688033013No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC129870.1560902229613081No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC129440.15557340771626796No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCTGGAATTCTCGGGTGCCAA127750.15354220361366835No Hit
TCCTGTACTGAGCTGCCCCGATTGGAATTCTCGGGTGCCAAGGAACTCCA125630.15099418426602862RNA PCR Primer, Index 1 (100% over 28bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA125460.15078986195984995No Hit
TCCTGTACTGAGCTGCCCCGAGTTTTGGAATTCTCGGGTGCCAAGGAACT120470.144792401325547RNA PCR Primer, Index 1 (100% over 25bp)
CATTGCACTTGTCTCGGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG119360.1434582968557922RNA PCR Primer, Index 1 (100% over 29bp)
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGGTGGAATTCTCGGGTGCCA111860.13444407746555725No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGGCCTGGAATTCTCGGGTGCC111040.1334585228122249No Hit
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG109890.13207634250572223RNA PCR Primer, Index 1 (100% over 29bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGCGTGGAATTCTCGGGTGCCAAGGA107050.12866295809661993RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGATGGAATTCTCGGGTGCCAA106370.1278456688719053No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA105930.12731683466767818No Hit
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG104980.1261750335449151RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG103740.12468468260572957RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT102590.12330250229922689RNA PCR Primer, Index 1 (100% over 30bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA100790.12113908964557052RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC99940.12011747811467723No Hit
TAGCAGCACGTAAATATTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT97420.11708870039955828RNA PCR Primer, Index 1 (100% over 30bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG97370.11702860560362338RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA96010.11539402715419411RNA PCR Primer, Index 1 (100% over 28bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC95790.11512961005208054RNA PCR Primer, Index 1 (100% over 26bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA93300.11213688921452256RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA92820.11155997917354753RNA PCR Primer, Index 1 (100% over 28bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA91090.10948069923420001No Hit
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG88790.10671633862119462RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATTCAGGATAGGTTGGAATTCTCGGGTGCCAAGGAACTCCAG88080.10586299251891904RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT87340.10497358953908253RNA PCR Primer, Index 1 (100% over 30bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA85140.10232941851794695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)