FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22073305
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA11936175.4075137366153365RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC9610854.354060255136238RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA4527782.051246970039149RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC4077851.8474125193304762RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA3937641.783892353229387No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC3452301.5640159006546595RNA PCR Primer, Index 31 (100% over 50bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA3317051.5027427927082055RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC3229821.463224469557232RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3115061.4112340675761967RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2760511.2506101827524243RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2272661.0295966100228306RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2051990.9296251739374779RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT1860860.8430364188779161RNA PCR Primer, Index 31 (100% over 39bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC1854750.8402683694172667RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1645620.7455249678287869RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC1590610.7206034619645767RNA PCR Primer, Index 30 (100% over 36bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1439960.6523536008767151RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1422960.6446519902660702RNA PCR Primer, Index 1 (100% over 30bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1405670.6368189992391262RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA1380430.6253843726619099No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1315660.5960412362353531No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1150290.5211226864305096RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC1147350.5197907608307863RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCG1082990.49063336913072153RNA PCR Primer, Index 31 (100% over 45bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC1046420.4740658455994696RNA PCR Primer, Index 31 (100% over 44bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC962230.4359247516400467RNA PCR Primer, Index 1 (100% over 27bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC932900.42263720815709294RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA883010.40003524619444164RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATAT856900.3882064783683277RNA PCR Primer, Index 31 (100% over 41bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCT846160.3833408726060733RNA PCR Primer, Index 31 (100% over 43bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC796770.3609654286025586RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT788680.3573003680237282RNA PCR Primer, Index 1 (100% over 30bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA776970.3519953174207487RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA707980.32074036941907885RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC658930.2985189576277771RNA PCR Primer, Index 2 (100% over 34bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG612890.27766118395047773Illumina Small RNA Adapter 2 (100% over 21bp)
TCACAGTGAACCGGTCTCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG601600.272546408433173RNA PCR Primer, Index 1 (100% over 29bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA584140.26463640130012245No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG582850.26405198496555RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG560060.2537272963881032No Hit
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG556630.252173383188426Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA556300.2520238813353959RNA PCR Primer, Index 31 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC554630.2512673113518796RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA550510.24940080336859388RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA544510.2466825878589545No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG540700.24495652101033352RNA PCR Primer, Index 1 (100% over 29bp)
TCACAGTGAACCGGTCTCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT536450.2430311183576723RNA PCR Primer, Index 1 (100% over 30bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATC536300.24296316296993134RNA PCR Primer, Index 31 (100% over 42bp)
CTGGCCCTCTCTGCCCTTCCGTTTGGAATTCTCGGGTGCCAAGGAACTCC530200.24019964386846462RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG510730.23137903453968492RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA489940.22196041779878453No Hit
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG486910.22058771896641668RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC479250.21711746383244376RNA PCR Primer, Index 1 (100% over 26bp)
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGT471800.2137423462413082RNA PCR Primer, Index 31 (100% over 46bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG446550.20230318930490923RNA PCR Primer, Index 1 (100% over 29bp)
CGAGAATTCTACCACTGAACCACCAATGCTGGAATTCTCGGGTGCCAAGG440060.199362986195316Illumina Small RNA Adapter 2 (100% over 21bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA429920.1947692019840255RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG422350.19133972008269717RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG418260.18948680317695968RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA402880.18251911075391744RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT401880.18206607483564424RNA PCR Primer, Index 1 (100% over 30bp)
TCACAGTGAACCGGTCTCTTTTTGGAATTCTCGGGTGCCAAGGAACTCCA380660.17245265264988638RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC379550.1719497827806031RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG368800.1670796466591659RNA PCR Primer, Index 31 (100% over 37bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC365500.16558462812886426RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA363580.16471479916577966No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA358510.16241790706013443RNA PCR Primer, Index 7 (100% over 35bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA351430.15921041275875997RNA PCR Primer, Index 1 (100% over 28bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC336430.15241487398466155RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG331150.15002284433617893RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG323730.1466613178225916RNA PCR Primer, Index 1 (100% over 29bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC321930.14584585316969978No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG318050.14408807380679967RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA316050.14318200197025321RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA313500.14202676037865647No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA313110.1418500763705299No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC312050.1413698582971603RNA PCR Primer, Index 1 (100% over 26bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC311410.14107991530946545No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA306690.13894158577521581RNA PCR Primer, Index 31 (100% over 40bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG306450.13883285715483024RNA PCR Primer, Index 1 (100% over 29bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG302800.13717927605313296RNA PCR Primer, Index 1 (100% over 29bp)
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTGGAATTCTCGGGTGCCAAG298020.13501376436378693No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA290750.13172019323794057RNA PCR Primer, Index 1 (100% over 28bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA286330.12971777447917293No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG273090.1237195789212354RNA PCR Primer, Index 1 (100% over 29bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA265130.12011341301178052RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC261950.11867275879167166RNA PCR Primer, Index 1 (100% over 27bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA259690.11764889761637418RNA PCR Primer, Index 1 (100% over 28bp)
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG258630.11716867954300456No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT255960.11595907364121502RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA252250.11427831038442136RNA PCR Primer, Index 1 (100% over 28bp)
ACCACAGGGTAGAACCACGGACTGGAATTCTCGGGTGCCAAGGAACTCCA249120.11286030796022616RNA PCR Primer, Index 1 (100% over 28bp)
GGCGAGAATTCTACCACTGAACCACCAATGCTGGAATTCTCGGGTGCCAA244900.11094849638511314No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA242120.10968905653231359RNA PCR Primer, Index 1 (100% over 28bp)
AAGGTTACTTGTTAGTTCAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG240030.10874221146312253RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA225740.10226832819099814RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC220890.10007110398737298RNA PCR Primer, Index 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph