FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29205612
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA17473825.983035041347533RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA9015453.0868896019025387No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC8589522.9410511924899914RNA PCR Primer, Index 1 (100% over 27bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC8354402.8605461169586173No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA7052372.414731114006445RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC6061152.075337438571738RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA4398161.5059297507616003RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA4336311.4847523140415617No Hit
CGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACT3911331.3392391845786351RNA PCR Primer, Index 1 (100% over 25bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCGTATGC3834511.3129360206524692RNA PCR Primer, Index 19 (100% over 50bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA3489161.1946881989666918RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG3438661.1773970016447524RNA PCR Primer, Index 1 (100% over 29bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA3221241.1029524051747315RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG3107491.0640044112069968No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2850550.9760281688327573RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2740420.9383196626730506RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAAC2553590.8743490805808144RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2342610.8021095397692743RNA PCR Primer, Index 1 (100% over 26bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGG2109410.7222618721360813No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC2090000.7156158891654111RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC2048480.7013994433672542RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1994930.6830639262070591RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1795380.614738016789376RNA PCR Primer, Index 1 (100% over 30bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA1779800.6094034256155975No Hit
CGCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAA1633180.5592007453909886RNA PCR Primer, Index 1 (100% over 23bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1494490.5117132967458445RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCC1464400.5014104823415445No Hit
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG1329660.45527551348692846No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT1203050.4119242562011712No Hit
GGCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGA1122300.3842754604834167RNA PCR Primer, Index 1 (100% over 22bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1096200.37533882186752326RNA PCR Primer, Index 1 (100% over 26bp)
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC1075250.3681655429785207RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1047580.3586913364458858RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1028750.35224394544445775RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGA1008360.3452624105257579RNA PCR Primer, Index 1 (100% over 22bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1003370.34355383479038204No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT996120.3410714351748561RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA991580.33951693941561645RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA933650.31968171048769667RNA PCR Primer, Index 1 (100% over 28bp)
AGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCTGGAATTCTCGGGTGCCA859130.2941660664395596No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT821140.28115829245420365RNA PCR Primer, Index 1 (100% over 30bp)
GGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTC792580.2713793499687663RNA PCR Primer, Index 1 (100% over 26bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG768490.26313093524628073RNA PCR Primer, Index 1 (100% over 29bp)
CGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAAC763400.26138811951620805RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCC732280.2507326331665298RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCCA722490.24738053768570234No Hit
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGG718910.24615474587555297No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG709160.24281634639260427RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC704370.24117625064662232RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC695420.2381117711212489RNA PCR Primer, Index 1 (100% over 26bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC684840.23448917968231586RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG681540.2333592598573178No Hit
GGGCCCTTCCCGTGGATCGCCTCAGCTTGGAATTCTCGGGTGCCAAGGAA669450.22921964449846144RNA PCR Primer, Index 1 (100% over 23bp)
GCGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAA654920.2242445732690005RNA PCR Primer, Index 1 (100% over 23bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA643580.22036175787037093RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC636990.2181053422198446RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG635540.2176088622967394RNA PCR Primer, Index 1 (100% over 29bp)
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG631530.21623583850939335Illumina Small RNA Adapter 2 (100% over 21bp)
GGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCA616930.21123679928364453RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA605890.20745670386910572No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG604340.20692598395130357RNA PCR Primer, Index 15 (97% over 36bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA575880.1971812814605631RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGGT571260.1955993937055659No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT538630.18442688343596428RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT497190.17023782963356493RNA PCR Primer, Index 1 (100% over 30bp)
GGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGAACTCC492980.16879632585682505RNA PCR Primer, Index 1 (100% over 27bp)
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT484730.16597152629433RNA PCR Primer, Index 1 (100% over 25bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAA484260.16581059831925454RNA PCR Primer, Index 19 (100% over 39bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA479230.16408832658599998No Hit
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG474200.1623660548527454RNA PCR Primer, Index 1 (100% over 29bp)
GGCCCGGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAA463700.15877085540956992RNA PCR Primer, Index 1 (100% over 23bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCATGGAATTCTCGGGTG446420.15285418432594394No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA444410.15216596043253605RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTGGAATTCTCG442760.15160100052003705No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG438760.15023140073216065RNA PCR Primer, Index 6 (100% over 34bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTTGGAATTCTCGG433930.14857760898829991No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC428960.14687588125186352RNA PCR Primer, Index 1 (100% over 27bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA407110.13939444241058876RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG398830.13655937084968464RNA PCR Primer, Index 1 (100% over 29bp)
CCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAGGAA396610.13579924296741325RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC395380.13537809103264126RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG394610.13511444307347506RNA PCR Primer, Index 1 (100% over 29bp)
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA392090.13425159520711294RNA PCR Primer, Index 1 (100% over 28bp)
AGCTACATTGTCTGCTGGGTTTTGGAATTCTCGGGTGCCAAGGAACTCCA389090.13322439536620564RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC384260.1315706036223449RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC378430.1295744119315151RNA PCR Primer, Index 1 (100% over 27bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA342060.11712132586024905RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG341910.1170699658682037RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC338780.11599825403419042No Hit
GCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTCC337870.11568667008244854RNA PCR Primer, Index 1 (100% over 27bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG332660.11390276635873954RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA329420.11279339053055967No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC328230.11238593459366646No Hit
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC326940.11194423866207631RNA PCR Primer, Index 1 (100% over 31bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA320590.10976999899882255RNA PCR Primer, Index 19 (100% over 37bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG319800.10949950304071697RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA314790.1077840793064018RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC308860.10575364762087505No Hit
TGTAAACATCCCCGACTGGAAGTGGAATTCTCGGGTGCCAAGGAACTCCA308200.10552766365587544RNA PCR Primer, Index 1 (100% over 28bp)
GGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACT306560.10496612774284614RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCCCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT299310.10248372812732018RNA PCR Primer, Index 1 (100% over 30bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG298460.10219268817239645Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)