FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7186169
Sequences flagged as poor quality0
Sequence length76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2628903.658277449361405RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2126302.9588783675975336RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC1822792.536525372559426RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC1259661.7528950404589707RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA1223981.703244106839124No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1001821.3940946838294508RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA762261.060732081307857RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC761131.0591596162016228RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG759661.057114020001478RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC665410.9259592976452404RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA602100.8378595048349128RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC560000.7792747429123918No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC450730.6272187587016114RNA PCR Primer, Index 26 (100% over 39bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC442330.6155296375579256RNA PCR Primer, Index 1 (100% over 27bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC430830.5995266740874031No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA422040.5872948437477604RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA392430.5460906917162677RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA367820.51184434988935RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC310770.43245573545515RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA308430.42919948027940896No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT295580.41131790805365137RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG265570.3695571312057927RNA PCR Primer, Index 15 (100% over 36bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC244480.34020908776289566RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG240200.3342532022277795No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC239010.33259724339909064No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC231800.3225640810840936RNA PCR Primer, Index 1 (100% over 27bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA230690.32101944721867803RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG216060.30066089456009176RNA PCR Primer, Index 1 (100% over 29bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC215850.30036866653149963No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC203870.28369775328133806RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA199950.27824283008095135RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA196240.2730801349091567No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAT195240.2716885728682417RNA PCR Primer, Index 26 (100% over 41bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA195010.2713685135988313No Hit
CAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA193220.2688776175455935RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC191430.26638672149235565RNA PCR Primer, Index 1 (100% over 26bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA179690.2500497831320137No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC176020.24494275044185573No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG165920.23088797382861437Illumina Small RNA Adapter 2 (100% over 21bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC148740.20698093796569492RNA PCR Primer, Index 1 (100% over 26bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA133300.18549522005396757No Hit
GTGAAATGTTTAGGACCACTAGTGGAATTCTCGGGTGCCAAGGAACTCCA123110.17131520285704388RNA PCR Primer, Index 1 (100% over 28bp)
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC122730.17078640928149616No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT118410.16477486126474342RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG116610.16227004959109645RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA114160.15886072259085474RNA PCR Primer, Index 1 (100% over 28bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCT111920.15574362361920518RNA PCR Primer, Index 26 (100% over 43bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG111050.15453296464360913No Hit
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCG109650.15258477778632815RNA PCR Primer, Index 26 (100% over 45bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA107600.14973207560245244RNA PCR Primer, Index 1 (100% over 34bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG104860.14591919561034536RNA PCR Primer, Index 26 (100% over 38bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATC102800.1430525778060605RNA PCR Primer, Index 26 (100% over 42bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA102670.14287167474074156No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT96280.13397959329929482RNA PCR Primer, Index 1 (100% over 25bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG96090.13371519651152095RNA PCR Primer, Index 1 (100% over 29bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA93330.1298744852785956RNA PCR Primer, Index 26 (100% over 40bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC90890.12647907389876303No Hit
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA90880.12646515827835386RNA PCR Primer, Index 1 (100% over 28bp)
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG88650.12336197492711345Illumina Small RNA Adapter 2 (100% over 21bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT87780.12215131595151743RNA PCR Primer, Index 1 (100% over 30bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTC87460.12170601609842462RNA PCR Primer, Index 26 (100% over 44bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG87190.12133029434737758RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG76820.10689979598308917RNA PCR Primer, Index 1 (100% over 29bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGGCCTGGAATTCTCGGGTGCC75310.10479853730130755No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA75070.10446456241148797No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT74750.10401926255839515No Hit
TGAGGTAGTAGTTTGTGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG73450.10221023190520569RNA PCR Primer, Index 1 (100% over 29bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCGCG72610.10104131979083708RNA PCR Primer, Index 26 (97% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT72440.10080475424388154No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA72280.10058210431733515RNA PCR Primer, Index 1 (97% over 37bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG71970.10015072008465149RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)