FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19248555
Sequences flagged as poor quality0
Sequence length76
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC1539239679.96650138153227RNA PCR Primer, Index 32 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATATCGTATGC2484901.2909540482389457RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAGCTCGTATGC1190920.6187061833992213RNA PCR Primer, Index 32 (98% over 50bp)
AGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC1051190.546113721263752RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCACTCTCGTATGC1001250.5201689165758157RNA PCR Primer, Index 32 (98% over 50bp)
TGGAAATCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC825660.4289464845542951RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATGTCGTATGC793730.41235822637075875RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCATCTCGTATGCC432400.22464023922834728RNA PCR Primer, Index 32 (97% over 39bp)
TGGAATTATCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC403480.2096157347915207RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCCATCTCGTATGC375530.19509516428635812RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGG344410.17892771691173703RNA PCR Primer, Index 32 (97% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTAGTATGC316750.16455780706655643RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGCTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC299020.1553467260269667RNA PCR Primer, Index 32 (98% over 50bp)
TGGAATTCTCGGGTGCCAAGGACCTCCAGTCACCACTCAATCTCGTATGC284430.14776693627132012RNA PCR Primer, Index 32 (98% over 50bp)
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT281980.14649411345423072RNA PCR Primer, Index 1 (100% over 30bp)
GGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC274080.14238990926851391RNA PCR Primer, Index 32 (98% over 50bp)
ACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC272080.14135087023415524RNA PCR Primer, Index 30 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAACTCAATCTCGTATGC224930.1168555249991493RNA PCR Primer, Index 32 (98% over 50bp)
TCGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC223350.11603468416200592RNA PCR Primer, Index 32 (98% over 50bp)
TTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA216780.11262144093413765RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAAACTCGTATGC206670.10736909861545452RNA PCR Primer, Index 32 (98% over 50bp)
TCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC205390.10670411363346496RNA PCR Primer, Index 1 (100% over 33bp)
CTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC204730.10636123075212658RNA PCR Primer, Index 2 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)