FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13773564
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC7204055.2303456098944325No Hit
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG2911742.1140062223546496No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGG2307621.6753978853984344No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2221351.612763406769664RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA2197641.5955492710528663No Hit
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG1785071.296011693124597No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT1581351.1481051672609937No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATCTCGTATGC1468011.065817097158005RNA PCR Primer, Index 28 (100% over 50bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1124340.8163028828268413No Hit
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCC1077940.7826151604624628No Hit
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGG911090.6614773053655539No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA870540.6320368497216842No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC824650.5987194018919141RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG633780.46014234224344547No Hit
CGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACT616930.4479087620313813RNA PCR Primer, Index 1 (100% over 25bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA604570.43893504978087006RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC578530.42002926766086107RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCTGGAATTCTCGGGTGCCA562400.408318427968244No Hit
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGGT553170.40161718492032994No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA551020.40005622364698057RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA527500.38298003334503694RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA522000.3789868765992593No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTTGGAATTCTCGG521600.37869646519956635No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC515700.3744128970540958RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA487540.3539679345157143RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA481050.3492560095556967RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA477930.3469908006380919No Hit
CGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCCA404050.2933518151148098No Hit
CGCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAA403400.2928798965903088RNA PCR Primer, Index 1 (100% over 23bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCATGGAATTCTCGGGTG388860.2823234422114712No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC371520.26973410803478315RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGA368290.2673890359822628RNA PCR Primer, Index 1 (100% over 22bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC355030.2577618980824426No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC345770.2510388741795515No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTGGAATTCTCG330380.23986529557636643No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAA325500.23632227650011284RNA PCR Primer, Index 2 (97% over 36bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC303790.22056019778177965No Hit
GCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAAC302700.21976882671761647RNA PCR Primer, Index 1 (100% over 24bp)
GGCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGA302370.21952923731286977RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAGATGTCGTATGC289880.21046114135745841RNA PCR Primer, Index 28 (98% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA284930.20686730028625852No Hit
GCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTGGAATTCTC280250.20346948690985137No Hit
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG275470.199999070683521Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC255560.18554384326380594RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCG248070.18010588980455602No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC241400.1752632797146766RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG228350.16578860779969512RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTC220210.15987873581594422No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA203050.147420086769118No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC202460.14699172995457094No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC192110.13947733498751666RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT190150.13805431912902136RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTTGGAATTCTCG181450.13173787118570038No Hit
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA180950.13137485693608422No Hit
CCGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA179270.13015512905737397No Hit
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG177130.12860142806901684Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAAC174990.12704772708065973RNA PCR Primer, Index 1 (100% over 24bp)
GCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTGGAA173510.12597320490179592No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA173490.1259586843318113RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGGTG172370.12514553241267112No Hit
CAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA172360.1251382721276788RNA PCR Primer, Index 1 (100% over 28bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTTGGAATT172220.12503662813778627No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGATTTCTCGGGTGC165890.12044086773764583No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT165640.12025936061283776No Hit
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC165320.12002703149308343RNA PCR Primer, Index 1 (100% over 26bp)
GGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCA160870.11679620467149969RNA PCR Primer, Index 1 (100% over 28bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAAAAG160600.11660017697670698RNA PCR Primer, Index 28 (100% over 39bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC157920.11465442059876442No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT151360.10989167364380055RNA PCR Primer, Index 1 (100% over 25bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTGGAATTC140390.10192714100722225No Hit
GGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGAACTCC138420.1004968648637346RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)