FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14061140
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA10599347.538037456422453No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4608173.27723783420121RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC3753412.6693497113320825RNA PCR Primer, Index 18 (100% over 50bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3283892.3354365293283474RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2462311.7511453552130196RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2392411.7014338808944367RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1839801.3084287618215877No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1771711.2600045231041013RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1387450.9867265385310152No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1385860.9855957625057428RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1297120.922485659057516RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA1121280.7974317871808403No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1091180.7760252724885749RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1051840.7480474556117072RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG967410.688002537489848RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC836790.5951082202438779RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC826310.5876550549955408RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG778000.5532979545044001No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC772800.549599819075836RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA770410.5479000991384767RNA PCR Primer, Index 1 (100% over 28bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG720190.5121846450572286Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA698800.49697250720780817No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC684540.48683108197486125RNA PCR Primer, Index 1 (97% over 36bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC597240.4247450775683906RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC444730.3162830325279458RNA PCR Primer, Index 1 (100% over 26bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC431330.30675322200049215RNA PCR Primer, Index 1 (100% over 26bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA427940.30434232217302437No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC417440.2968749333268853No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA411900.2929349967356843No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT407610.2898840350071189RNA PCR Primer, Index 1 (100% over 30bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA401370.285446272492842RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA368930.26237559685772277RNA PCR Primer, Index 1 (100% over 28bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA356220.25333650045444395No Hit
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA356200.25332227685664177RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC344650.2451081491258888No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC340290.24200740480501579No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA330430.23499517108854615No Hit
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG329160.23409197262810838RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTTGGAATTCTCGGGTGC322300.22921327858196422No Hit
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC300600.21378067496661013RNA PCR Primer, Index 1 (100% over 26bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC291990.20765741611277608No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA290950.20691778902706326No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA290520.2066119816743166RNA PCR Primer, Index 1 (100% over 28bp)
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG283470.2015981634490518No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC283160.20137769768311817No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC281380.20011179747872507RNA PCR Primer, Index 1 (100% over 27bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA262930.18699052850622352No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA251840.17910354352492047No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC240930.1713445709238369RNA PCR Primer, Index 1 (100% over 26bp)
CGAGAATTCTACCACTGAACCACCCATGCTGGAATTCTCGGGTGCCAAGG237280.16874876432494093Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT229310.16308066060077633No Hit
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG226680.161210257489791RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCTCAGCTTGGAATTCTCGGGTGCCAAGGAACTC211760.15059945352937243RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT203850.14497402059861433RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG203350.14461843065356011RNA PCR Primer, Index 6 (100% over 34bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG202300.14387169176894618RNA PCR Primer, Index 1 (100% over 29bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC200120.1423213196085097No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT197630.14055048168213957RNA PCR Primer, Index 1 (100% over 25bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC194990.1386729667722532No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA194720.1384809482019239No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA187650.13345290637885693RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA186980.13297641585248424No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC185360.13182430443050847RNA PCR Primer, Index 18 (100% over 37bp)
GGGGATGTAGCTCAGTGGTAGAGCGCATGCTTGGAATTCTCGGGTGCCAA184350.1311060127414989No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG183180.130273932270072No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG182960.13011747269424812RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG180650.1284746471480975RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC172760.1228634378151416RNA PCR Primer, Index 18 (100% over 39bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG170060.12094325211184867Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG169480.12053076777558576RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA169420.12048809698217924RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT166290.11826210392613971RNA PCR Primer, Index 1 (97% over 35bp)
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA164330.11686819134152707RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT161690.11499067643164068RNA PCR Primer, Index 1 (100% over 30bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC159140.11317716771186404No Hit
CGAGAATTCTACCACTGAACCACCAATGCTGGAATTCTCGGGTGCCAAGG158290.11257266480527184Illumina Small RNA Adapter 2 (100% over 21bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA158280.11256555300637075RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA157160.11176903152944925No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA154330.10975639244044223No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC153920.10946480868549775RNA PCR Primer, Index 1 (100% over 26bp)
TTCGAGACCCGCGGGCGCTCCCTGGCCCTTTGGAATTCTCGGGTGCCAAG151300.10760151737341354No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA150290.10688322568440398RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG150050.10671254251077791RNA PCR Primer, Index 1 (100% over 29bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG149640.10642095875583345RNA PCR Primer, Index 1 (100% over 29bp)
CTGGCCCTCTCTGCCCTTCCGTTTGGAATTCTCGGGTGCCAAGGAACTCC149620.10640673515803127RNA PCR Primer, Index 1 (100% over 27bp)
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACT148630.10570266706682388RNA PCR Primer, Index 1 (100% over 25bp)
GCGGATGTAGCGCAGTTGGTAGCGCATCACCTGGAATTCTCGGGTGCCAA147120.10462878543276008No Hit
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA145060.1031637548591366RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGAATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA142170.10110844497672308No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)