FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18631988
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA9073894.870060027947635RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC8340854.476629117622875No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA4433642.3795850448164737No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC4214502.261970112904753RNA PCR Primer, Index 1 (100% over 27bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG3856222.0696771595172776No Hit
CGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACT3055251.6397874451185779RNA PCR Primer, Index 1 (100% over 25bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGG2802611.5041926819617961No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA2308881.2392021720924251RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2301771.2353861541774287RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC2205021.1834593281189318RNA PCR Primer, Index 1 (100% over 27bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC2143321.1503442359451928RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2127691.1419554370687657RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1897861.0186030605000391RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1851220.9935708417158706RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT1804290.9683829766313719No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA1685200.9044660183336315No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1436290.7708731886259266RNA PCR Primer, Index 1 (100% over 28bp)
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCC1301960.6987767488901345No Hit
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGG1200870.6445205954404866No Hit
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG1138350.6109653999347788No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1077900.5785211969866018RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1072940.5758591085395719RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAAC966480.5187208149769096RNA PCR Primer, Index 1 (100% over 24bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG847950.4551044150522209RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG841530.45165872798973467No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC818790.43945391119831123RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC766220.4112389939280768RNA PCR Primer, Index 1 (100% over 26bp)
CCCTTCCCGTGGATCGCCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC731230.39245946272614607RNA PCR Primer, Index 1 (100% over 27bp)
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGGT704670.3782044084614052No Hit
CGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAAC695380.3732183597370286RNA PCR Primer, Index 1 (100% over 24bp)
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG691810.3713023001088236Illumina Small RNA Adapter 2 (100% over 21bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC685530.36793175263960026No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTTGGAATTCTCGG682740.3664343278881459No Hit
CGCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAA667750.3583890242952067RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCCA620110.3328200941305887No Hit
AGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCTGGAATTCTCGGGTGCCA608560.3266210776863961No Hit
GCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTGGAATTCTC562280.3017820749991895No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT557540.29923806305585854RNA PCR Primer, Index 1 (100% over 25bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT553700.29717709135493214RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT550320.29536300688901257RNA PCR Primer, Index 9 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGC547380.2937850754304908RNA PCR Primer, Index 34 (100% over 50bp)
CGCGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGA543350.29162212856727904RNA PCR Primer, Index 1 (100% over 22bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA543090.29148258360836216RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCATGGAATTCTCGGGTG540290.2899797917431033No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG524710.2816178284356989RNA PCR Primer, Index 1 (100% over 29bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTTGGAATTCTCGGGTGCCAAG515140.27648150052479636No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTGGAATTCTCG512600.2751182536184545No Hit
CCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAGGAAC499990.2683503231109852RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC475430.25516869160714356RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA449920.2414771842918748RNA PCR Primer, Index 1 (100% over 28bp)
GGCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGA435690.2338397813480773RNA PCR Primer, Index 1 (100% over 22bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC414250.22233268935123834RNA PCR Primer, Index 1 (100% over 26bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC385090.20668218549732856RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA381570.20479296143814607RNA PCR Primer, Index 1 (100% over 28bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA381270.2046319480240112No Hit
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC365180.19599626191257746RNA PCR Primer, Index 1 (100% over 31bp)
GCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCTGGAA348050.18680239596547613No Hit
GCGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAA329100.17663171530595662RNA PCR Primer, Index 1 (100% over 23bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC314750.16892990699650515No Hit
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACT311460.16716412655482604RNA PCR Primer, Index 1 (100% over 25bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA310160.16646640176024158RNA PCR Primer, Index 1 (100% over 28bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC308850.16576330985185264RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCG307860.16523196558520756No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT305300.16385798445125663RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGGTG290600.1559683271586478No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA288790.15499687956003408No Hit
CGGGCCCTTCCCGTGGATCGCCTCAGCTGGAATTCTCGGGTGCCAAGGAA288710.1549539426495981RNA PCR Primer, Index 1 (100% over 23bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT276630.1484704691737672RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTTGGAATTCTCG274470.14731117259199608No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG257590.13825148449000718RNA PCR Primer, Index 1 (100% over 29bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA257050.13796166034456442No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTGGAATTCTCGGGTGCCAAGG256520.13767720331292613Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC247800.1329970800754058RNA PCR Primer, Index 1 (100% over 27bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG246630.1323691277602798Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC244360.13115079292665924RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC239850.12873022460083164No Hit
GGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGAACTCC233010.12505911875855652RNA PCR Primer, Index 1 (100% over 27bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGTGGAATTCTCGGGTGCCA232990.12504838453094752No Hit
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC228370.12256877795327047RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA224440.12045950222810363RNA PCR Primer, Index 1 (100% over 28bp)
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC217000.11646636955755876RNA PCR Primer, Index 1 (100% over 26bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG214510.1151299582202393RNA PCR Primer, Index 1 (100% over 29bp)
AGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTTGGAATT213560.11462008240881219No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC207990.11163060001970805RNA PCR Primer, Index 1 (100% over 26bp)
CCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAAGGAA206120.11062694973826732RNA PCR Primer, Index 1 (100% over 23bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCATGGAATTCTCGGGT203760.10936031088040632No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT201680.1082439512090712RNA PCR Primer, Index 1 (100% over 30bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTGGAATTCTC201370.10807757068113182No Hit
GGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTC195710.10503978426778722RNA PCR Primer, Index 1 (100% over 26bp)
GGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCA191830.10295734411164283RNA PCR Primer, Index 1 (100% over 28bp)
GGGCCTGTAGCTCAGCTGGCTAGAGCGTACGACTGGAATTCTCGGGTGCC189720.10182488309889422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)