FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20029066
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA10197225.09121094313634RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA8677714.332558492742497RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA5913122.952269466783923RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA5444812.718454270408815No Hit
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC5410992.701568810048357RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3742081.8683247636210296RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2795921.3959312930518077RNA PCR Primer, Index 1 (100% over 29bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGCATCTCGTATGC2742231.3691252502737772RNA PCR Primer, Index 24 (100% over 50bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2046181.0216053010160333RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2042611.01982289139194RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC2017011.007041466636537No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1861290.9292944563665626RNA PCR Primer, Index 1 (100% over 27bp)
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG1778100.8877598186555478No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1742400.8699357224146149No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1740170.8688223404925621RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1408430.703193049541102RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1285030.6415825880248236No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1230190.6142023796816087RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1171940.5851196456190219No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC1066120.5322864281339929RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA1034770.5166341755526693No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGT978450.48851504109078275RNA PCR Primer, Index 9 (97% over 36bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG976350.4874665648413161No Hit
TTTGGTCCCCTTCAACCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC963300.48095103386248766RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC948220.4734219758425081RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT904030.4513590399073027No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC891560.4451330880830888RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT830110.4144526759260766RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC781300.3900830922420446RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC778320.388595254516611RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA651210.3251324849596082RNA PCR Primer, Index 1 (100% over 28bp)
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG648630.32384435699597774Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA648590.3238243860197974No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC631370.3152268807741709No Hit
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA611920.3055159936064917RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG595740.2974377337415534RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC592720.2959299250399395RNA PCR Primer, Index 1 (100% over 26bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG587240.293193901303236Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACT566160.2826691968562089RNA PCR Primer, Index 1 (100% over 25bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA557460.27832550953698987RNA PCR Primer, Index 1 (100% over 28bp)
TTGGTCCCCTTCAACCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA539770.26949334532124464RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA520240.2597425162012048No Hit
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA514820.2570364489287718No Hit
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG488680.24398541599493453RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT469220.23426953608321027No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA466090.23270680719710046No Hit
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG458000.22866767726463133RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT453570.2264558916526612RNA PCR Primer, Index 1 (100% over 30bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGG444980.22216712451793807No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA436550.21795824128793626No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG409050.2042281951639682RNA PCR Primer, Index 1 (100% over 29bp)
GCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTCC406600.2030049728729238RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG383880.19166145840250362RNA PCR Primer, Index 1 (100% over 29bp)
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA363930.18170093403257048RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG352900.176193937350848RNA PCR Primer, Index 1 (100% over 29bp)
GTCAGGATGGCCGAGTGGTCTAAGGCGCCAGATGGAATTCTCGGGTGCCA348180.17383736216157059No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG339820.1696634281398843RNA PCR Primer, Index 1 (100% over 29bp)
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT338080.1687946906760405RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA336130.16782110558725005RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG329900.16471062604716566RNA PCR Primer, Index 1 (100% over 29bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG327870.16369709900601456RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT325120.16232409439361775RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCATGACAGAACTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG298840.1492031630431494RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT293610.14659195790757293RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC292570.14607271252688467RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG291210.1453936993367539RNA PCR Primer, Index 1 (100% over 29bp)
ACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG290460.14501924353337295RNA PCR Primer, Index 1 (100% over 29bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC288560.1440706221648079No Hit
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC285810.1426976175524111RNA PCR Primer, Index 1 (100% over 27bp)
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGGTGGAATTCTCGGGTGCCA279230.13941239197074892No Hit
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA276660.13812925675116353RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT275530.1375650766740696RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG273820.136711317442361RNA PCR Primer, Index 6 (100% over 34bp)
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACT261330.130475380130057RNA PCR Primer, Index 1 (100% over 25bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC259090.12935700546395923RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG257300.12846330427989003RNA PCR Primer, Index 2 (97% over 35bp)
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCC252930.1262814751321904No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC246620.12313105363974536No Hit
CCCTTCCCGTGGATCGCCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC244850.12224733794376633RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCC244180.12191282409274601No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGTAGC244000.1218229546999346RNA PCR Primer, Index 24 (100% over 39bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTGGAATTCTCGGGTGCCAA239540.11959619085582922No Hit
AGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCTGGAATTCTCGGGTGCCA237910.1187823735764813No Hit
GGTCCCATGGTGTAATGGTTAGCACTCTGGACTGGAATTCTCGGGTGCCA231570.11561697385190103No Hit
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC228790.114228991007369RNA PCR Primer, Index 1 (100% over 31bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA221830.1107540411519938RNA PCR Primer, Index 1 (100% over 28bp)
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA221760.11071909194367824No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA218250.10896663878385543RNA PCR Primer, Index 1 (100% over 28bp)
GGCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGA211060.10537685581544343RNA PCR Primer, Index 1 (100% over 22bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG209290.10449314011946437RNA PCR Primer, Index 1 (100% over 29bp)
GGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCA204100.10190190596006823RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA200950.10032919158586827RNA PCR Primer, Index 1 (100% over 28bp)
GGTTCCATGGTGTAATGGTTAGCACTCTGGACTCTGGAATTCTCGGGTGC200470.10008953987170445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)