FastQCFastQC Report
Wed 16 Nov 2022
EGAF00007271842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22001636
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA9109044.140164849559369RNA PCR Primer, Index 1 (100% over 28bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC6653133.023925129931247No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA5532422.514549372601201RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4911462.232315815060298RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC4769872.1679615097713643RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA3624171.6472275061727228No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC3608311.6400189513179837RNA PCR Primer, Index 1 (100% over 27bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG2984431.3564582197432955No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGC2609291.1859527173342927RNA PCR Primer, Index 35 (100% over 50bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2508111.1399652280403147RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2304421.047385748950669RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2055110.9340714481414018RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG1967410.8942107759622966RNA PCR Primer, Index 1 (100% over 29bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC1820600.8274839198321435RNA PCR Primer, Index 1 (100% over 26bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA1777730.8079990051648887RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1635420.7433174514840624RNA PCR Primer, Index 1 (100% over 27bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGG1539210.6995888851174522No Hit
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCC1489170.6768451218809365No Hit
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG1388220.630962170267702No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1356340.6164723386933589RNA PCR Primer, Index 1 (100% over 29bp)
CGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACT1242080.5645398369466706RNA PCR Primer, Index 1 (100% over 25bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1187380.5396780493959631RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1147550.5215748501611426No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT1140090.5181841932118139No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC1081050.49134982507664426RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1017800.46260196287221556No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA1009580.4588658770647782No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA1006490.4574614360495738RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA972340.4419398630174593No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC872220.39643415607821164RNA PCR Primer, Index 1 (100% over 26bp)
GCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAAC845930.3844850446575882RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC818760.37213596298020746RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA790560.35931873429775857No Hit
TGAGATGAAGCACTGTAGCTCATGGAATTCTCGGGTGCCAAGGAACTCCA790270.35918692591769086RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA788710.3584778877352575RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA700370.3183263280966925RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT674950.30677264181627223RNA PCR Primer, Index 9 (97% over 36bp)
CGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCCA669770.30441827144126915No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTT639250.2905465757182784RNA PCR Primer, Index 35 (100% over 39bp)
GGCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGA639220.2905329403686162RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGG638980.2904238575713188No Hit
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC586790.2667028942756802RNA PCR Primer, Index 1 (100% over 26bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT570790.2594307077891844RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC563070.2559218778094502RNA PCR Primer, Index 1 (100% over 26bp)
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGGT562500.2556628061658688No Hit
CGCGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAA558520.2538538497773529RNA PCR Primer, Index 1 (100% over 23bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG549080.24956325975032037Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT512890.23311448294117765RNA PCR Primer, Index 1 (100% over 30bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTC507730.23076920279928273RNA PCR Primer, Index 35 (100% over 44bp)
GGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTC492700.22393789261853075RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC488270.22192440598508223RNA PCR Primer, Index 1 (100% over 27bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCG479340.21786561690230669RNA PCR Primer, Index 35 (100% over 45bp)
CCCTTCCCGTGGATCGCCTCAGCTGGAATTCTCGGGTGCCAAGGAACTCC476590.21661570984994025RNA PCR Primer, Index 1 (100% over 27bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCATGGAATTCTCGGGTG455160.20687552507458992No Hit
GGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCA440290.20011693675870287RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT423510.19249023118099037RNA PCR Primer, Index 1 (100% over 30bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCT423160.19233115210159826RNA PCR Primer, Index 35 (100% over 43bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA412430.187454242039092RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGGAATTCTCGGGTGCCA400740.18214100078739598No Hit
GGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGAACTCC374270.17011007726879945RNA PCR Primer, Index 1 (100% over 27bp)
AGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCTGGAATTCTCGGGTGCCA363220.1650877234765633No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT347680.1580246123515542RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG345840.1571883109056072RNA PCR Primer, Index 1 (100% over 29bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTAT333150.1514205579985052RNA PCR Primer, Index 35 (100% over 41bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG328680.14938889089884044RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG320260.145561902760322RNA PCR Primer, Index 1 (100% over 29bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC313660.14256212583464248No Hit
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGT312420.14199853138193905RNA PCR Primer, Index 1 (100% over 30bp)
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC311320.14149856856099247RNA PCR Primer, Index 1 (100% over 24bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTTGGAATTCTCGG310280.14102587643937023No Hit
CGCGGGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGA302810.1376306743734875RNA PCR Primer, Index 1 (100% over 22bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG297270.13511267980253833RNA PCR Primer, Index 1 (100% over 29bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG297000.1349899616555787RNA PCR Primer, Index 1 (100% over 29bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA294250.13374005460321223No Hit
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT293220.13327190759814406RNA PCR Primer, Index 1 (100% over 30bp)
AAATGGATTTTTGGAAGTAGGAGTGGAATTCTCGGGTGCCAAGGAACTCC291120.1323174331217915RNA PCR Primer, Index 1 (100% over 27bp)
CCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCAAG287000.13044484510151882No Hit
TACCCTGTAGAACCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG284230.12918584781604422RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT283450.12883132872482755No Hit
TACCCTGTAGATCCGAATTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG270770.12306812093427962RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC269180.1223454474021841RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC266140.12096373196974988No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC264400.12017288168934347No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATC257590.11707765731602868RNA PCR Primer, Index 35 (100% over 42bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA255810.11626862656940604No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGTGGAATTCTCGGGTGCCAAGGA254420.11563685536839169RNA PCR Primer, Index 1 (100% over 22bp)
GGCCCGGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAA243040.11046451272987154RNA PCR Primer, Index 1 (100% over 23bp)
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA240420.10927369219270786RNA PCR Primer, Index 1 (100% over 28bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA238340.1083283079494634No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC237090.10776016838020591No Hit
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG235660.10711021671297534RNA PCR Primer, Index 1 (100% over 29bp)
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG234870.1067511525052046Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATCAGATGGAATTCTCGGGTGCCAA232860.10583758407783858No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTCTGGAATTCTCG228100.10367410859810607No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC226110.10276963040384815RNA PCR Primer, Index 1 (100% over 26bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG220070.10002438000519598RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)