FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35824203
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA781812521.823583904992947No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA13377103.7340956336139564No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA8351742.3313121578727096RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA7680282.1438802141669417RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC6381331.781290151800446RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA5699981.591097504667445RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC5099591.423504104194586RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA4551891.270618637349727RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG3412600.952596209886372RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA3355090.936542817156323RNA PCR Primer, Index 1 (100% over 28bp)
GCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTCC3096540.864370939389775RNA PCR Primer, Index 1 (100% over 27bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA3004810.8387653453169636No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2724190.7604328280520295RNA PCR Primer, Index 1 (100% over 26bp)
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA2641980.7374846552762109RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2343980.6543006692989095RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC2179610.6084182807918993RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA2173020.6065787423100522No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC1920850.5361877834379176RNA PCR Primer, Index 1 (100% over 27bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA1739200.4854818403077941RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG1593330.4447635583127977RNA PCR Primer, Index 1 (100% over 29bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC1587700.44319199508779017No Hit
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG1490530.416067874559554Illumina Small RNA Adapter 2 (100% over 21bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT1321630.36892097780933186RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATCTCGTATGC1283420.3582550043053296RNA PCR Primer, Index 35 (100% over 50bp)
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCC1210620.33793354732832437RNA PCR Primer, Index 1 (100% over 27bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1207490.3370598363346701RNA PCR Primer, Index 1 (100% over 30bp)
GGCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGA1064420.29712314883878926RNA PCR Primer, Index 1 (100% over 22bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1023880.2858067770551657RNA PCR Primer, Index 1 (100% over 29bp)
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1015700.28352340455417807RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC1015070.2833475457918771RNA PCR Primer, Index 1 (100% over 26bp)
AGTAAGGTCAGCTAATTAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA1011730.2824152152107892No Hit
GGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCA989970.27634110938909096RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC911470.25442854932460046No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGG885550.24719321738993047Illumina Small RNA Adapter 2 (100% over 21bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA881560.24607944522869077RNA PCR Primer, Index 1 (100% over 28bp)
CCCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAAC847900.23668356278575128RNA PCR Primer, Index 1 (100% over 24bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC830500.23182651125553305RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC804470.22456047382268352RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA792980.2213531449673842RNA PCR Primer, Index 1 (100% over 23bp)
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC771640.21539627832055327RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG754190.21052526974570795RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG683820.19088212513757807No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAG669320.18683458219572951No Hit
TTTGTTCGTTCGGCTCGCGTGATGGAATTCTCGGGTGCCAAGGAACTCCA644370.17987001692682458RNA PCR Primer, Index 1 (100% over 28bp)
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC631500.1762774736398183No Hit
CCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAACTCC622790.17384615646578375RNA PCR Primer, Index 1 (100% over 27bp)
ACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCAGT615060.17168839736643968RNA PCR Primer, Index 1 (100% over 30bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC606240.16922637469422558RNA PCR Primer, Index 1 (100% over 27bp)
CCCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAACTC597930.16690671387720754RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA581990.16245720805009953RNA PCR Primer, Index 1 (100% over 28bp)
GGACACGGACAGGATTGACAGTGGAATTCTCGGGTGCCAAGGAACTCCAG558610.15593089398248441RNA PCR Primer, Index 1 (100% over 29bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC544270.15192801358344243No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCAA535620.1495134448629604No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC507520.1416695857825504No Hit
GCCCCGGCCGGGGGGCGGGCGCCGGCTGGAATTCTCGGGTGCCAAGGAAC490940.137041429784216RNA PCR Primer, Index 1 (100% over 24bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA481050.13428072635698274RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTGGAATTCTCGGGTGCCA470160.1312408820372082No Hit
CCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAACTC460190.12845784733857166RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTCGTCCCGGCCTCCTGGAATTCTCGGGTGCCAAGGAACTCCA457620.12774045524474056RNA PCR Primer, Index 1 (100% over 28bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAG456660.12747247998790093No Hit
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG449540.12548499683300698RNA PCR Primer, Index 1 (100% over 29bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT444270.12401392432931446RNA PCR Primer, Index 1 (100% over 30bp)
GGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGAACTCC439910.12279687003783447RNA PCR Primer, Index 1 (100% over 27bp)
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG437280.12206272949045092RNA PCR Primer, Index 1 (100% over 29bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA431080.12033205595669498No Hit
GGCCCGGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAA430900.12028181059603754RNA PCR Primer, Index 1 (100% over 23bp)
CCCCCACTGCTAAATTTGACTGGCTTGGAATTCTCGGGTGCCAAGGAACT422240.11786445046663006RNA PCR Primer, Index 1 (100% over 25bp)
CCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAACT413880.11553083260498495RNA PCR Primer, Index 1 (100% over 25bp)
CCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG413870.11552804119605954RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA401620.11210856526242888No Hit
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA397810.1110450384618466RNA PCR Primer, Index 1 (100% over 28bp)
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG386400.10786004087795058RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG386260.1078209611529948RNA PCR Primer, Index 1 (100% over 29bp)
AGCTCGGTCTGAGGCCCCTCAGTTGGAATTCTCGGGTGCCAAGGAACTCC383630.10708682060561125RNA PCR Primer, Index 1 (100% over 27bp)
GGCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTC372090.10386553470568487RNA PCR Primer, Index 1 (100% over 26bp)
AACATTCAACGCTGTCGGTGATGGAATTCTCGGGTGCCAAGGAACTCCAG367870.10268756013916067RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG367820.1026736030945336RNA PCR Primer, Index 1 (100% over 29bp)
GCCCCGGCCGGGGGGCGGGCGCCGGCGGCTGGAATTCTCGGGTGCCAAGG363460.10145654880305362Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCCACAACCGCGCTTGACTAGTGGAATTCTCGGGTGCCAAGGAACTCC361450.10089547560904566RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT358860.10017250069736373RNA PCR Primer, Index 9 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)