FastQCFastQC Report
Wed 2 Nov 2022
EGAF00007271852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5690824
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA66480811.682104384180567No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1189882.0908747133982706RNA PCR Primer, Index 1 (100% over 28bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA653311.148005982964857No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC640881.1261638033437689No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA582201.0230504404985992No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC468200.822727956443566No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA368340.6472524892704467RNA PCR Primer, Index 1 (100% over 28bp)
GCCGCCGGTGAAATACCACTACTCTCATCGTTTTTTCACTGTGGAATTCT361350.6349695580112827No Hit
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG352290.6190491921732248No Hit
GGCCCGGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGA325450.5718855476816714RNA PCR Primer, Index 1 (100% over 22bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC311800.5478995660382399RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC283120.4975026463654473RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA265190.46599578549608983RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA254260.4467894280336205RNA PCR Primer, Index 1 (100% over 28bp)
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG247000.4340320487859052Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA243730.4282859564801161No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA210550.36998157033146695RNA PCR Primer, Index 1 (100% over 28bp)
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGG203490.35757563403823417No Hit
CGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACT202480.3558008471180975RNA PCR Primer, Index 1 (100% over 25bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGGG201980.3549222397318912No Hit
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC197740.3474716490968619RNA PCR Primer, Index 1 (100% over 26bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC195570.34365849304072665RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGC193050.33923031181424695RNA PCR Primer, Index 36 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC181340.31865332682929576RNA PCR Primer, Index 1 (100% over 31bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG166580.2927168367884862RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA165930.291574647186418RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC156650.2752676940984293No Hit
GCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTCC156050.2742133652349818RNA PCR Primer, Index 1 (100% over 27bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG148220.26045437356699136RNA PCR Primer, Index 1 (100% over 29bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACA147460.25911889033995783RNA PCR Primer, Index 36 (100% over 39bp)
CCGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA135040.23729428286659365No Hit
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA133570.23471117715114717No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC132900.23353384325363077RNA PCR Primer, Index 1 (100% over 27bp)
GGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACTCCA120620.2119552458484044RNA PCR Primer, Index 1 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA120590.211902529405232RNA PCR Primer, Index 1 (100% over 28bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA117720.2068593230084079RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA116900.2054184068950296RNA PCR Primer, Index 1 (100% over 28bp)
GGCCCGGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAA113950.2002346233164125RNA PCR Primer, Index 1 (100% over 23bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGT110570.19429523738565804No Hit
GGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTGGAATTCTCGGGTGCC107830.1894804689092476No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCA106250.18670406956883573RNA PCR Primer, Index 2 (97% over 36bp)
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTGGAATTCTCGG106070.18638777090980146No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGGAATTCTCGGGTGCCA102320.1797982155132543No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC97890.17201375407146663RNA PCR Primer, Index 1 (100% over 26bp)
CCCCCCACTGCTAAATTTGACTGTGGAATTCTCGGGTGCCAAGGAACTCC97260.1709067087648467RNA PCR Primer, Index 1 (100% over 27bp)
CAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA96900.17027411144677818RNA PCR Primer, Index 1 (100% over 28bp)
CGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCG96640.1698172356059509RNA PCR Primer, Index 36 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG94130.16540662652719534No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT90170.1584480560284416RNA PCR Primer, Index 1 (100% over 25bp)
GGCCCTGGCGGAGCGCTGAGAAGACGGTCGTGGAATTCTCGGGTGCCAAG87220.1532642724498245No Hit
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTTGGAATTCTCGGGTGCCAAG81470.14316028750845222No Hit
CCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGCC81090.14249254589493543No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC81020.14236954086086656No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC80870.14210595864500467No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG80800.14198295361093577RNA PCR Primer, Index 1 (100% over 29bp)
CCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTC78300.13758991667990433RNA PCR Primer, Index 36 (100% over 44bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT78200.1374141952026631RNA PCR Primer, Index 1 (100% over 30bp)
GGCTGGTACGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA76000.13354832270335545No Hit
GGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGC73120.12848754415880723No Hit
CGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAATGGAATTCTCGGGTGCC72370.1271696330794978No Hit
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC70750.12432294514818944RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGACA70530.12393635789825867No Hit
GGACACGGACAGGATTGACAGATTGGAATTCTCGGGTGCCAAGGAACTCC67030.11778610619481468RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC66490.11683721021771187RNA PCR Primer, Index 1 (100% over 26bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGTGGAATTCTCGGGTGCCA66030.1160288914224021No Hit
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCATGGAATTCTCGGGTG65950.1158883142406091No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAAT64690.11367422362736924RNA PCR Primer, Index 36 (100% over 41bp)
CCCGGACACGGACAGGATTGACAGATGGAATTCTCGGGTGCCAAGGAACT64510.113357924968335RNA PCR Primer, Index 1 (100% over 25bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC64330.11304162630930072No Hit
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCT62950.11061666992337137RNA PCR Primer, Index 36 (100% over 43bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGCACTTGGAATTCTCGGGTGCCA62100.10912303736682069No Hit
GCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA60640.10655750379909834RNA PCR Primer, Index 1 (100% over 28bp)
GGACACGGACAGGATTGACAGTGGAATTCTCGGGTGCCAAGGAACTCCAG60190.10576675715151268RNA PCR Primer, Index 1 (100% over 29bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCACCTTGGAATTCTCGG60060.10553831923109905No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA58200.10226989975441166No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)