FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16509451
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA9068195.492726560077619RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA6086953.6869487664974443RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC5544323.3582703628364143RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC4517972.7365961472613476RNA PCR Primer, Index 1 (100% over 27bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTGGAATTCTCGGGTGCC3264681.97746127354568No Hit
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG3170951.9206877321359748RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA2628521.592130471207068RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTTGGAATTCTCGGGTGCCAAGGAACTCC2423341.4678501423215102RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC2180391.3206920084744187RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA2144901.2991952306590935RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC1843551.116663419031923RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCA1811201.0970685821109376No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1734811.0507981155763446RNA PCR Primer, Index 1 (100% over 28bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCTGGAATTCTCGGGTGCCA1445870.8757832104774411No Hit
AAACCGTTACCATTACTGAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA1277740.7739445727177724RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCAACAATCTCGTATGC1177990.713524635071148RNA PCR Primer, Index 36 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1168260.707631041153337RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1123020.6802285551469883RNA PCR Primer, Index 1 (100% over 29bp)
CAACGGAATCCCAAAAGCAGCTGTTGGAATTCTCGGGTGCCAAGGAACTC1079440.653831553817265RNA PCR Primer, Index 1 (100% over 26bp)
GCTCCCATCGTCTAGAGGCCTAGGACTCCGCCCTTGGAATTCTCGGGTGC1013400.6138302236700663No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT958560.5806128865217869RNA PCR Primer, Index 1 (100% over 30bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC855550.5182183223415485RNA PCR Primer, Index 1 (100% over 26bp)
CGGACTGTGGCGCAGTTTGGCAGCGCACTACACTTGGAATTCTCGGGTGC853840.5171825519818921No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC832580.5043050795571579RNA PCR Primer, Index 1 (100% over 26bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA688190.41684608410055546RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA684570.4146534006491191RNA PCR Primer, Index 1 (100% over 28bp)
ACGGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAA662840.4014912428038946No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTTGGAATTCTCGGGTGC637930.38640291551790545No Hit
CGGGCTGTAGCGCAGTTTGGTAGCGCACCTCACTTGGAATTCTCGGGTGC623140.3774444104773684No Hit
TCCTGTACTGAGCTGCCCCGAGTTTGGAATTCTCGGGTGCCAAGGAACTC601120.3641065956705647RNA PCR Primer, Index 1 (100% over 26bp)
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA540340.32729131937821554RNA PCR Primer, Index 1 (100% over 28bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGGCCTGGAATTCTCGGGTGCC535260.3242142939822772No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA478480.2898218723323992No Hit
TCCTCGATAGCTCAATGGTAGAGCACTCGGCTGGAATTCTCGGGTGCCAA471320.2854849625223758No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG466900.28280770814244516RNA PCR Primer, Index 1 (100% over 29bp)
GCTGCTGTAGCTCAGTCGGTAGAGCGCATCCTTGGAATTCTCGGGTGCCA455880.27613274360243717No Hit
CGGGCCGCCGGTGAAATACCACTACTGGAATTCTCGGGTGCCAAGGAACT446820.27064497783724RNA PCR Primer, Index 1 (100% over 25bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA434450.263152299855398RNA PCR Primer, Index 1 (100% over 28bp)
GACTCTTAGCGGTGGATCACTCGGCTGGAATTCTCGGGTGCCAAGGAACT421440.25527196513076056RNA PCR Primer, Index 1 (100% over 25bp)
AAGCTGCCAGTTGAAGAACTGTGGAATTCTCGGGTGCCAAGGAACTCCAG404250.2448597473047408RNA PCR Primer, Index 1 (100% over 29bp)
GGCCCTGGCGGAGCGCTGAGAAGACGGTCTGGAATTCTCGGGTGCCAAGG399130.24175849336237773Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTGC394480.23894192484050497No Hit
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTGGAATTCTCGGGTGCCA391060.23687038412119216No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC381640.23116456143817257RNA PCR Primer, Index 1 (100% over 26bp)
CAAAGTGCTGTTCGTGCAGGTAGTGGAATTCTCGGGTGCCAAGGAACTCC377730.22879622102515706RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG370700.224538053991014No Hit
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGCCCTGGAATTCTCGGGTGCC357480.21653051939764684No Hit
CAAAGAATTCTCCTTTTGGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA354750.21487692110416026RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT352690.21362915096328766RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT320730.19427054236994312RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG307830.18645683614797365RNA PCR Primer, Index 1 (100% over 29bp)
CCACTACTCTCATCGTTTTTTCACTGGAATTCTCGGGTGCCAAGGAACTC304510.1844458667947226RNA PCR Primer, Index 1 (100% over 26bp)
TCCGTCGTAGTCTAGGTGGTTAGGATACTCGGTGGAATTCTCGGGTGCCA299340.18131432716932866No Hit
ATGACCTATGATTTGACAGACCTGGAATTCTCGGGTGCCAAGGAACTCCA297980.18049055659088845RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC294170.17818278754393468RNA PCR Primer, Index 1 (100% over 31bp)
CCCATAAAGTAGAAAGCACTATGGAATTCTCGGGTGCCAAGGAACTCCAG290180.1757659900380697RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCA288800.17493010518641716RNA PCR Primer, Index 2 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG288240.17459090553647122RNA PCR Primer, Index 1 (100% over 29bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGGAATTCTCGGGTGCCA287000.17383982059730516No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGTGGAATTCTCGGGTGCCAAGGAAC281950.17078096661118533RNA PCR Primer, Index 1 (100% over 24bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG271230.1642877161693626RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG252540.15296692785241617RNA PCR Primer, Index 1 (100% over 29bp)
GCCCCGTTCGTCTAGCGGCCTAGGACGCCGGCCTTGGAATTCTCGGGTGC250880.15196144317579063No Hit
GCATCCTTAGCTCAGCTGGATAGAGTACTCGGCTGGAATTCTCGGGTGCC240700.14579527811070156No Hit
GCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTCC237430.1438145944404814RNA PCR Primer, Index 1 (100% over 27bp)
GGCTCCGTGGCGCAATGGATAGCGCATTGGACTGGAATTCTCGGGTGCCA235660.1427424812611879No Hit
ACTGGACTTGGAGTCAGAAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG235290.1425183672067593RNA PCR Primer, Index 1 (100% over 29bp)
GGGGATGTAGCTCAGTGGTAGAGCATGCTTGGAATTCTCGGGTGCCAAGG229690.13912637070729972Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT222900.13501357495170493RNA PCR Primer, Index 1 (100% over 30bp)
TCAGTGCATGACAGAACTTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG221820.13435940419823772RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA220670.13366283348852726RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTATGGAATTCTCGGGTGCCAAGGAACTCC215730.1306706080050754RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA204300.12374730086421408RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA203690.12337781553123724RNA PCR Primer, Index 1 (100% over 28bp)
CGGATAGCTCAGTCGGTAGAGCATCAGACTGGAATTCTCGGGTGCCAAGG201760.12220878816624489Illumina Small RNA Adapter 2 (100% over 21bp)
AAGGTTACTTGTTAGTTCAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG200330.12134261763156146RNA PCR Primer, Index 1 (100% over 29bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTGGAATTCTCGGGTGCCA198590.12028867586208651No Hit
CTAGACTGAGGCTCCTTGAGGTGGAATTCTCGGGTGCCAAGGAACTCCAG197350.11953759092292046RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC190020.11509770979059207RNA PCR Primer, Index 1 (100% over 26bp)
TATGCGAAACCGAAAGGTGAGGTATATGGGCTGGAATTCTCGGGTGCCAA189380.11471005304779668No Hit
CCCATAAAGTAGAAAGCACTACTGGAATTCTCGGGTGCCAAGGAACTCCA189000.11447988185676193RNA PCR Primer, Index 1 (100% over 28bp)
TCCTCGATAGCTCAATGGTAGAGCACTCGGCTTGGAATTCTCGGGTGCCA187370.11349256858995493No Hit
CCACTACTCTCATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACT185950.11263245519187767RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA184350.11166331333488921RNA PCR Primer, Index 1 (100% over 28bp)
AAACCGTTACCATTACTGAGTTTAGTGGAATTCTCGGGTGCCAAGGAACT175930.10656320431248743RNA PCR Primer, Index 1 (100% over 25bp)
GCGCCCGTAGCTCAACGGATAGAGCATCTGACTTGGAATTCTCGGGTGCC175680.106411775897333No Hit
CCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTCGCCTGGAATTCTCGGGTG175630.1063814902143021No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGTGGAATTCTCGGGTGCCAAGGA170520.1032862934085452RNA PCR Primer, Index 1 (100% over 22bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCA169850.1028804652559313RNA PCR Primer, Index 1 (100% over 28bp)
GGGCCTGTAGCTCAGCTGGCTAGAGCGTACGACTGGAATTCTCGGGTGCC169330.10256549415241004No Hit
ACAGTAGTCTGCACATTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG168390.10199612331142931RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCCCGACTGGAAGCTGGAATTCTCGGGTGCCAAGGAACTCC167860.10167509507130187RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG167610.10152366665614743Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTGTACTGAGCTGCCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT165150.10003361105102768RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)