FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36362807
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA14193433.9032822741104667No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC4676921.2861823346035965No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCTGGAATTCTCGGGTGCCAAG3994381.098479553572418No Hit
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTC3538890.9732169466455106No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATGGAATTCTCGGGTGCCA3383450.9304699716938795No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3123060.8588610884742753RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3003550.8259950888829897No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATT2976010.8184214161464488No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2515530.6917865279212356No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC2400390.6601223057394882RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA2305600.6340544611971237RNA PCR Primer, Index 4 (100% over 39bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT1911470.525666239132749RNA PCR Primer, Index 4 (100% over 46bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1844370.5072133182677564RNA PCR Primer, Index 1 (100% over 28bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCT1792740.4930147444337837No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1751920.481788988402353No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1726830.47488908103271565No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1686720.4638585794545509No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC1651900.4542828610563535No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGA1643860.4520718106278209RNA PCR Primer, Index 1 (100% over 22bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT1587250.43650370555826445RNA PCR Primer, Index 4 (100% over 46bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG1565310.43047006794607473RNA PCR Primer, Index 2 (97% over 35bp)
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG1272780.35002248313778417No Hit
AGTGCGGTAACGCGACCGATCCCGGAGAAGCCGTGGAATTCTCGGGTGCC1205420.3314980606420181No Hit
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1049190.2885338307353445RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1045640.28755755846901476No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGG1016830.27963462776677284No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA970790.26697333899442915RNA PCR Primer, Index 1 (100% over 28bp)
GCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG838330.23054600817808152RNA PCR Primer, Index 2 (97% over 35bp)
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCG799020.21973551161768123No Hit
GCCGCCGGTGAAATACCACTACTCTGATCGTTTTTTCACTGTGGAATTCT768250.21127356862191637No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA752640.20698072071278764No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA739250.20329838672795528RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT735340.20222311220363157RNA PCR Primer, Index 1 (100% over 30bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG637340.17527249752748736RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT630050.17326770180310888RNA PCR Primer, Index 1 (100% over 30bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA618820.1701793813662405RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGCGGGGCGCGGGACATGTGGCGTACGGAATGGAATTCTCGGGTGCC617830.16990712515675702No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG607930.16718456306192203No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG606400.16676380346544753RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC595270.16370298365579972No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGA579220.1592891329869006RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC576650.15858236686733232RNA PCR Primer, Index 4 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA572680.15749059196667628No Hit
GCCGCCGGTGAAATACCACTACTTGGAATTCTCGGGTGCCAAGGAACTCC561030.15428676889548157RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA558100.15348100051791932No Hit
GCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC553390.15218572097583116RNA PCR Primer, Index 4 (100% over 38bp)
CTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC538590.14811562814718895RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC528980.1454728178712936RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT528570.14536006529968934RNA PCR Primer, Index 1 (100% over 25bp)
CACTCCCGACCCGGGGAGGTAGTGACGTGGAATTCTCGGGTGCCAAGGAA512780.14101771626156362RNA PCR Primer, Index 1 (100% over 23bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC500210.13756088741993983No Hit
CCACTACTCTGATCGTTTTTTCACTGACCCGGTGGAATTCTCGGGTGCCA495160.13617210574530178No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC477980.13144749798881036RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC468590.12886518909280023RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT462100.1270803983861862RNA PCR Primer, Index 4 (100% over 46bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC460920.12675589098498363RNA PCR Primer, Index 1 (100% over 26bp)
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC460590.12666513891515582RNA PCR Primer, Index 4 (100% over 38bp)
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA447790.12314505863092473RNA PCR Primer, Index 4 (100% over 39bp)
CCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCT443710.12202303304032606No Hit
AGCGCACGTGTTAGGACCCGAAAGATGGTGAACTGGAATTCTCGGGTGCC440700.12119526416098736No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGT438180.12050224835502936RNA PCR Primer, Index 4 (100% over 46bp)
GAAACTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCA432740.11900621423423115No Hit
AAGGGCTTCTGTCGTGAGTGGCACACGTAGGGTGGAATTCTCGGGTGCCA431210.11858545463775666No Hit
GGCGTCCAGTGCGGTAACGCGACCGATCCCGGATGGAATTCTCGGGTGCC429750.11818394548033653No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAG427960.11769168425308861No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA426370.11725442428028178RNA PCR Primer, Index 1 (100% over 28bp)
AGTGCGGTAACGCGACCGATCCCGGATGGAATTCTCGGGTGCCAAGGAAC419570.11538438162928401RNA PCR Primer, Index 1 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA417670.11486186971209347RNA PCR Primer, Index 1 (100% over 28bp)
GGCCACTGATTATCGAGGCGATTCTGATCTGGTGGAATTCTCGGGTGCCA414930.11410835252625025No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA392150.10784370964540774RNA PCR Primer, Index 4 (100% over 39bp)
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGTGGAATTC379000.1042273771659047No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC376970.10366911443332745RNA PCR Primer, Index 1 (100% over 26bp)
AAACTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAA376710.10359761280255399No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG372150.10234358420129668No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC372100.1023298338876864RNA PCR Primer, Index 4 (100% over 50bp)
GCCGCCGGTGAAATACCACTACTCTGATCGTGGAATTCTCGGGTGCCAAG369120.10151031519651385No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG367630.10110055585092756RNA PCR Primer, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)