FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27847988
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC3688181.3243972957759103No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2277940.8179908724465121No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA2100400.7542376131446192RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT1901580.6828428682172658RNA PCR Primer, Index 6 (100% over 39bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCTGGAATTCTCGGGTGCCAA1748650.62792687213166No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1372240.49276091328393273RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1350620.48499733625280217No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1309390.47019195785347223No Hit
GATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC1132510.40667569951552696RNA PCR Primer, Index 6 (100% over 36bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC1103940.3964164305155547No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1034250.37139128327691034RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG984530.3535372106595277No Hit
GGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTG978930.3515262933896696No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA903770.3245369108892176RNA PCR Primer, Index 6 (100% over 37bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC838520.301106133771675RNA PCR Primer, Index 1 (100% over 31bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA829490.2978635296740289RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC809630.29073195521342515RNA PCR Primer, Index 6 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC797350.2863223009145221No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC791640.2842718834840061No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA751540.26987227946234393No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC653680.23473150017157435No Hit
TGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGG652950.23446936274175356No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA586670.21066872048350493No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATGGAATTCTCGGGTGCCA576220.20691620522100196No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG574840.2064206577509298No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT520540.18692194208069898RNA PCR Primer, Index 6 (100% over 46bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT502360.18039364280105263No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT440160.15805809741084345RNA PCR Primer, Index 1 (100% over 30bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG437790.15720704849484995RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC434720.15610463492012422RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT423620.15211870961736984RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA404530.14526363628137157No Hit
GGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT403000.14471422495585679RNA PCR Primer, Index 6 (100% over 39bp)
CTGGTGCGGACCAGGGGAATCCGACTGTTTAATTTGGAATTCTCGGGTGC388080.1393565668011635No Hit
GCCGCCGGTGAAATACCACTACTCTGATCGTGGAATTCTCGGGTGCCAAG384390.13803151595727492No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA380990.13681060190057537RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG372410.1337295893692571RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA366080.1314565346695783RNA PCR Primer, Index 1 (100% over 28bp)
GCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT366060.13144935282218595RNA PCR Primer, Index 6 (100% over 39bp)
TACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC361050.1296503000504022RNA PCR Primer, Index 1 (100% over 27bp)
ACCCTGTAGATCCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCA360460.12943843555232787RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT349340.12544532840218114RNA PCR Primer, Index 6 (100% over 46bp)
ACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC327230.117505796109938RNA PCR Primer, Index 1 (100% over 33bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC324510.11652906486457835RNA PCR Primer, Index 1 (100% over 26bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT322830.11592578968362095RNA PCR Primer, Index 6 (100% over 39bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC321640.1154984697637761RNA PCR Primer, Index 1 (100% over 26bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA320800.1151968321732974RNA PCR Primer, Index 1 (100% over 28bp)
AGTAAGGTCAGCTAAATAAGCTATCGGGCCTGGAATTCTCGGGTGCCAAG317940.11416982799619133No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC317350.113957963498117RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT313570.1126005943409628RNA PCR Primer, Index 1 (100% over 30bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC309910.1112863162681627No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA303560.10900607972109153RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC298890.10732911835497774RNA PCR Primer, Index 1 (100% over 27bp)
CGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTG292810.10514583674770328No Hit
GCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTTGGAATTCTCGGGTGCCA290630.10436301538193711No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG287860.10336832951809662No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA282020.10127123007953034No Hit
GGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA280580.10075413706728113RNA PCR Primer, Index 6 (100% over 37bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC278640.10005749787022317RNA PCR Primer, Index 1 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)