FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73088815
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGACCATGAGTAGCGTAGTCATCTCGCTGTCTCTTATACACATCTCCGA44920836.146060789191889No Hit
GCATACATGAGTAGCGTAGTCATCTCGCTGTCTCTTATACACATCTCCGA13832611.8925754918861388No Hit
AACTCCATGAGTAGCGTAGTCATCTCGCTGTCTCTTATACACATCTCCGA8366291.1446744621594973No Hit
GGTGCGTAGTCATCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAGA4306500.5892146424866787No Hit
ATCGAATCGTCAGTACGTACGAGTCTTACCTCTCGTACTCGCAGTAGTCC2796660.38263857472583185No Hit
ACTTCAGTACAGTACGTACGAGTCTGCATACATGTACTCGCAGTAGTCCG2077600.284256900320521No Hit
AATGCAGCGGTCAGTACGTACGAGTCATGGAGAGTGTACTCGCAGTAGTC2066050.2826766311644265No Hit
AAGGAAGTCGTCAGTACGTACGAGTCCTGCTTAGGGTACTCGCAGTAGTC2053620.28097596054881996No Hit
TATCTAGGTGTCAGTACGTACGAGTCATGCGCCGTGTACTCGCAGTAGTC1811040.24778620367562396No Hit
AGTTGTGTAGTCATCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG1558380.21321730281165457No Hit
CCACTGAAGTCAGTACGTACGAGTCTTCACTCCTGTACTCGCAGTAGTCC1434240.19623248783004624No Hit
AGTAGCGTAGTCATCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG1430690.19574677739678772No Hit
AGTGTGCACCAGTACGTACGAGTCGAACGCTGTGTACTCGCAGTAGTCCG1280910.17525390170848987No Hit
TCGCATTCGGTCAGTACGTACGAGTCCACTGGTCCGTACTCGCAGTAGTC1261410.172585914821577No Hit
CTGAGTAGCGTAGTCATCTCGCTGTCTCTTATACACATCTCCGAGCCCAC1185900.1622546486764083No Hit
GTTGCATGGAGTACGTACGAGTCTGCCGAACTGTACTCGCAGTAGTCCGA1162070.15899423188076042No Hit
CCGCACTCACAGTACGTACGAGTCGTAGTTGGTGTACTCGCAGTAGTCCG1103820.15102447618011047No Hit
ATCGAATCGTCAGTACGTACGAGTCTTGCCAGATGTACTCGCAGTAGTCC1048320.1434309750404354No Hit
GGTACGAGCCAGTACGTACGAGTCCACAACGCTGTACTCGCAGTAGTCCG993080.13587304705925252No Hit
GTGCTCGGACAGTACGTACGAGTCCCTATTGGTGTACTCGCAGTAGTCCG946050.1294384099673801No Hit
TCATGCTCAGTCAGTACGTACGAGTCAGAGGACGTGTACTCGCAGTAGTC934470.1278540362160749No Hit
TCCAATCATAGTACGTACGAGTCATCGGTGGTGTACTCGCAGTAGTCCGA849460.11622298158753841No Hit
GGACACTGGTCAGTACGTACGAGTCCGGAGACTTGTACTCGCAGTAGTCC805880.11026037294488904No Hit
AAGACTGGTCAGTACGTACGAGTCTCGCTGATTGTACTCGCAGTAGTCCG783910.10725444105230054No Hit
AGCCGGATCGTCAGTACGTACGAGTCGACTTGATTGTACTCGCAGTAGTC762830.10437027881762756No Hit
TCATGCTCAGTCAGTACGTACGAGTCTGAGATCATGTACTCGCAGTAGTC744720.10189247150880748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)