FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90506123
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGCCTCCCCATCCTTGTAGAGGGGAACATCTGAGGCCACAGATTTTG16267431.797384470882705No Hit
AGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA15008821.6583209513902168No Hit
TGGTCCTTTCGGCAAGAGACTTGATGAAACGCAGGTAAGTGGGCTCTGAA14038051.551060805024208No Hit
GGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT12634811.396017151237381No Hit
CCAAATTTTATGACAAAAGTTGTGGACAGGTTTTGAAAGATATTTGTGTT12310601.3601952654628682No Hit
CATTCTGGAAAATGTGGAGGCTTGCACTTTCAAGAAAAGTTAAAACTCCA11790991.3027836801715615No Hit
GGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC11570141.278382016209003No Hit
TGTCCTCACAGAGTTCAAGCTGAAGAAGATGTGGAAAAGTCCCAATGGAA11238261.2417126739590867No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG11100751.2265192267709886No Hit
GATGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTAT10932741.2079558418384577No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC9928481.0969953933392993No Hit
AGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT9856251.0890147178219092No Hit
TCTGCATTATAAAAAGGACAGCCAGATATCAACTGTTACAGAAATGAAAT9720951.07406545300808No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA9671141.0685619579572532No Hit
TGTGTTCACAGAGACCTGGCCGCCAGGAACGTGCTTGTCACCCACGGGAA9402391.038867834389503No Hit
CAGAAAGGACAAGGGTGGTTGGGAGTAGATGGAGCCTGGTTTTTTAAATG9278221.0251483206279868No Hit
GCCAGTGAAAATTAAGTGCTATGGTAAGATAACCTCTTTTGTTGCTGGTG9150801.0110697151396044No Hit
GTGAAAGAATTTTCCCATTCAACCTCTCTTCCCCAGACTGTTTTCGTGAG9123141.008013568319571No Hit
GCTTCATGTCTTAGCACCTCACCTAACTCTGGCTTGAATAACCTTCCCAT9070151.0021587158252265No Hit
CTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCCACCTGTCCCA9014580.9960187997446317No Hit
TATCTGAAGTGTGTGAAGAGTTTGGATAAGGACTAACTGGATTGGGCCGT8983010.9925306379547382No Hit
GCTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTTGTGTTTGT8753130.967131251440303No Hit
TCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAAGCATTGG8527170.9421649847933493No Hit
CTATGAATGTGCCGTTATGCAGGCAGCTGTGTAATTGGATGGGTTTTTTT8331340.9205277746788469No Hit
TTTGTTGCGTTTTCTCCAGAAGGTCCAGTATTCACTCTGTGCGCTTTTGT8292560.9162429817041219No Hit
TCTTCACTGATTGTTAGTTTGCTGCGGATTAAAACACAGGTGATCATATT8208110.9069121212937162No Hit
TCCTTGTTTTGGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGG8024640.886640564638925No Hit
GTGACGGACTTCAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGGTGAGT7991010.8829247939390796No Hit
TCTGTGGGGAGTAGAGTATATGTAGAGTGGTTGTTAGGACTGAAAATGAT7901010.8729807153489495No Hit
GATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG7819090.8639293940366886No Hit
AAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG7796420.8614245911295969No Hit
CATCGTTTTCACCACTCACAGTGCTGGCTTCATTAGACCCACAGCTCTCC7627700.8427827584659658No Hit
TGTGACTGGCCCTGACATTTCAACTTTTACTTGCTTCTGTGATTTGAGAG7395960.8171778609940015No Hit
CGCTGTTTCATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTG7350270.8121295837630786No Hit
TGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCTTT7307700.807426034589947No Hit
TTTTAAATCCCACCCCACTTTACATATAATTGACCTTTCTGATTCTCTTC7261780.8023523447137384No Hit
TTGGTCCACTGTACCTTGGGACTGCCCTTGATTCATTTCAACTTGGTACA7250600.8011170691733198No Hit
GAACAAACAGGTCTGAGGATTTACAGTGATAGCTTTTGTTTTCATTCTGT7191730.7946125368777537No Hit
CTTGAGTGTCAGGTGGCCTTGCTAATTCCTGGAGAGGTCAAGGTGACTTT7169750.7921839719065195No Hit
GTTAATCTGCCCTGTGCCTTTGCGTTAATTACCTTTCTTCCATGATTTCT6877550.7598988634172298No Hit
CAGAGGTGCTAGGAATCAAAATCTCCAAGAGTGACTATTACTGAGCGGAA6783970.7495592314787365No Hit
AAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT6620590.7315074141447867No Hit
ACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA6566240.7255022955739691No Hit
TGCTCCCCACAATAGGACATCGGCAGAAACTAGATGATCAGACCAAGCCC6563240.7251708262876313No Hit
TTGGGTCTTGTTTCCTGCAAAAAGTTCAGGATGTGTAGTCTGTTCTTTAT6530310.7215323984212648No Hit
GAATACTGTTGCTATGGTGATCTTCTCAACTATCTAAGAAGTAAAAGAGA6509120.7191911203620998No Hit
CTCATCCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAG6346730.7012486878926413No Hit
GGTTTTCTCCCCTCTAAATCTGGTATGGTGGAAATGGGTGCAAAGCCTTG6326920.6990598857051914No Hit
GGCTTCACAAAGTATTGGTATTATAGGAGTGAACCACTATGCCCAGCCAG6313910.697622413900107No Hit
GACTCCTCTTTGCATCGGGTAATTATTTATCACTGTATCTGGTCCCCTCC6275140.6933387258230032No Hit
AGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA6178340.6826433168505074No Hit
GTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCTC6008610.6638898895271428No Hit
AAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG5928850.6550772261010451No Hit
TTTGACTGCTAAAATGTGTGATATCCCTAGACAGGATTTACATTATGAAA5926650.6548341486243975No Hit
AAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG5877020.6493505417307511No Hit
ACTCCACAGGTAGTAGGGAAGAATGTACTCGTGTTAATTGTGTATGATCG5830990.6442646979807101No Hit
AGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC5382560.5947177739565753No Hit
TAGGCTAGAAATGTATGGTCAGAAAACACTGTGAAAAGCAAAGCTTACCA5276550.5830047542750229No Hit
TCAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCA5264070.5816258420438581No Hit
TCAGTGCTTCAGAGTCTCCGTTGATTTTCAGCTTTTCAATGTCCACCTGT5206800.575298093367672No Hit
CCTTAGGCATTTTGGGATCTGTTCAGTCCTAGTAGGAGAGGTTGCCTTTA5185220.572913724301283No Hit
TTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAATGACT5183090.5726783811079832No Hit
TCTATGGCAGTGGTGACCTCTCGCCCCTCGGACCTGCAGAGCACGGGCTT5035680.5563910852749708No Hit
GGCAAACCCTGAAGAACTGTAACCAGTTGCATTTACAAAATCATAATAAC4808380.5312767623467862No Hit
GGGCTGTTTCACTACCTCATCATTCGATGGGATGGGTATCCAATGCAAAG4786860.528899022666124No Hit
CTAGACCTTCTCTGTCCATTTAGGTGTGAAACCATTCCAGTGTAAAACTT4776600.527765397706849No Hit
GCTGCTGGAACCTGAACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTC4771560.5272085293058018No Hit
AGCAGGAACAATGTCTTTTCAAGTCCTTTGCCCATTTTTAAATTGAATTT4768870.526911311845719No Hit
TAGGACTCTGGGAAGGTGGTGCCTCAGGTTTACCCTCTATTTTCACTTCC4680120.5171053454582294No Hit
GTCATGTCTTCAGGGCTTAGGCTCTGTGAGGCCAGGTGTGGAGCCTCCCT4652730.5140790308739664No Hit
GTTCTGAGATGCTTTGATTCCCATGTGAGATTTAGCCCTCCTTGACTGAG4525530.5000247331332488No Hit
TTGACCCATGAGTTGGAGCCACGGCCACTCCCCCAATGTCAGGATCCAGA4502020.4974271188259827No Hit
CAGTGTGAGAACAGACTCAACAGCTGCTAAGCTGTCCTCAGCCCAACTTA4399570.48610744269755096No Hit
CTGCCAGTAATTTAAACATTCCCATAGAGGTATTTACCTACTACTTCCAA4265610.4713062341649526No Hit
TCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTA4175480.46134779190574765No Hit
CAGAACTGCCTATTCCTAACTGACTCATCATTTCATCTCTGAAGCAATTT4104920.45355163429108547No Hit
CAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAG4088730.4517628050424831No Hit
TTTTGCATACGGTGATGAGTGAAGAACCTCCAAACCTCTCTCTGAAGGTC4025010.4447223974006709No Hit
CCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTGTCTCCT3982590.44003542169185617No Hit
ACCATTAGGCATTAGCACTGCCTTGTTTTTAAGTAATACAATGTTCTTGT3943090.43567107608841005No Hit
CAGCAGACTTTGTGGTCACTAAAATGTTACTGACCTTTCAGAGGTAGGAT3933220.4345805421363591No Hit
AGCCCAGAGAGAGAAGGTTCACTAACTGTGCGTTTTATTCCTCCATTTTG3855150.42595460640823163No Hit
TTGATTTATCTTCATTAAAGTTAAGGCGACATAAATCTAAATTACTAAAG3652340.40354617775418355No Hit
CCTGATGTCTGCATTTGTCCTTTGACTGGTGTTTAGGTGGTGGCCCTAGG3622720.40027347100040955No Hit
CAGCTGTATAGGTACTTGAAGTGCAGTTTCTACTAAATGCATGTTGCTTT3533110.39037248341750314No Hit
GGCAGAAATGGGCTTGAATAGTTAGATGCTTATTTAACCTTGGCAATAGC3444520.38058419539195154No Hit
AGGTTGTCCTTTTATTCTTAAGAGTCAATTTTATTTTTTCTGTTTTAGTG3361740.3714378528842739No Hit
AAATCATGACTGATATGGTAGACAGAGCCTAAACATCCCCTTAAATTGGA3318930.36670778616823524No Hit
TTCAACAGATATGTTCAGGCCACCAACCTCATTCTGTTTTGTTCTCTATC3272860.3616175228277097No Hit
GGTCCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCA3162730.3494492853262536No Hit
GGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTG3157030.348819493682212No Hit
TGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA3032610.3350723574801674No Hit
CCATGATGCTCTCCTGCTTATGGTGCACCTCCTCCAGGAAGTCCAGCACG3028270.33459283191259886No Hit
ATCATCTCCCACTAGAGACAGAATGGGACCATTGTCTGCAGGAACGGTGG3022740.33398182352811645No Hit
CGTCAAATGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCA3018460.3335089273462747No Hit
GTTAATCACAAGGAAGCAGGACACCAATGAAACTTCAAGAAGATGCTCTG2924020.3230742742123646No Hit
AAAAGGTGACATGGAAAGCCCCTGTTTCATACTGACCAAAACTCAGCCTG2858230.3158051527629793No Hit
TTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGTAATGGG2814530.31097675015866055No Hit
AATAGGCGAATTGATCTAAAACAGATACATTCAAATTTTACAGGATCTGA2671310.29515240642889984No Hit
GCTCTTTATCTAAATAGAGCCATTCCTTTATGTTTTAGGCAAGATAAACA2563410.2832305610969547No Hit
CTTAAAGCCTCCCTTCCTCTTACTCTCTGCCTGCAGGATGTGCGACGTGT2547530.2814759836746073No Hit
GCCTGGCTTCTCTCATAATTTTGAATTTAAATAAATAATGCAGATTGACT2544050.2810914793024556No Hit
CACCCTGGACTCTTTTCTGGCTGCCGCTCTGGCACACCCCTTTTTTGTGG2532060.279766707054726No Hit
ACGTGAGGAATGTGATTTTCTCAATAAGGCATTAAGAGTGGTCTTAGGAA2525900.27908609012011265No Hit
GAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAGATCTGGAGGATCAC2491880.27532722841304336No Hit
TTTTGGACATAGGTCATTAGTAACAAGTAAGTTGTTACAATTGCTGCCAA2461740.2719970669829709No Hit
TTCCTGGAATGGTAGCCATCTGGTGATGGGACTATGAAGGTCGGGCACAA2457070.27148107979390523No Hit
CAGTGTGTCTCTTTGCAGTTATGATGGTTAACGGTGATCACAGGATAGGT2447840.2704612592896063No Hit
CTTAAAGCCTCCCTTCCTCTTACTCTCTGCCTGCAGGATGTGCGGCGTGT2407990.2660582422694208No Hit
GAGATGGCTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTG2373860.26228722668851917No Hit
CAGCAGTAGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACA2286520.2526370508656083No Hit
GGTCTCCTCTCTTTCGTGTCAAAGGACTTCTTTGCCAAGTTCACAGATAA2248740.2484627476529958No Hit
CTCCTGTTAAATTATAGTTTACACTGACACCTAGCTGTGATCCTGAAACT2236240.2470816256265888No Hit
GGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGG2142980.23677735041197157No Hit
GCCTCGTGACCACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGG2132030.23556748751683906No Hit
TTCTTTGTTTACATTTTAGGCTGCTGGTCTGGCTACTATGATCTCTACCA2001770.22117509110405714No Hit
TCTTTTAGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGA1949350.2153832177741168No Hit
TCAATTGTGTTACGAAAGGACTGACGATCTTCAAGCCGGGGCTTCTTTTC1947310.21515781865940717No Hit
AAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCAA1709640.1888977168981153No Hit
CACGTAATCAGCAATGAGTATTCTCTTCATTTCAGGTCAGTTGATTTATT1586970.17534393777976767No Hit
TAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT1459790.16129184983429243No Hit
TAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG1456560.16093496790266887No Hit
GCACTCGCTAAAATTGGAAACATGCATTTTAGCAAAAAGCACAACTGGCA1444980.15965549645740543No Hit
TGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTTCCTCCTC1411330.1559375159623178No Hit
TGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA1395910.15423376383054216No Hit
GGATCAGTGCATAACAGCCTAATCTCGTCGCCCACGCGGACTATTAAGTC1307620.14447862273362433No Hit
TGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT1304210.1441018526448205No Hit
TCCTTGTTTTGGAGATGCAGGGTATGAAGAGAGCTGTTGAACATGCCCGG1279730.14139706326830506No Hit
TCGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA1259290.13913865253072435No Hit
TAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG1055210.11658990187879333No Hit
TAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG1049710.11598220818717425No Hit
CGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT968240.10698060726786408No Hit
GGTATCAGGAATGGACTTAGTCTGTTGCCCTCAACATGGTTGGTTCTATC925560.10226490422090007No Hit
CGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC910090.10055562760101877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)