FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106015499
Sequences flagged as poor quality0
Sequence length140-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATAACTGTCAGTACGTACGAGTCGATGCGGTTGTACTCGCAGTAGTC19513421.8406195494113555No Hit
CATATGCGACAGTACGTACGAGTCTCCGGAGCTGTACTCGCAGTAGTCCG18018731.6996316736668853No Hit
TCGCAAGCATCAGTACGTACGAGTCTTGATGAGTGTACTCGCAGTAGTCC15781901.4886408259984703No Hit
ACCATGTCTCAGTACGTACGAGTCTAGATCTGTGTACTCGCAGTAGTCCG15462051.4584707090799998No Hit
ATAATCGAGGTCAGTACGTACGAGTCCAGCGACGTGTACTCGCAGTAGTC13436641.2674222285177377No Hit
GTGCGAGTAGTCAGTACGTACGAGTCAAGCATTGCGTACTCGCAGTAGTC13421421.2659865893759554No Hit
TTATCCAAGGTCAGTACGTACGAGTCCTGGACACTGTACTCGCAGTAGTC12233511.1539359919439705No Hit
GTCCTAGTTCAGTACGTACGAGTCGTAGTTGGTGTACTCGCAGTAGTCCG11197421.056205942114181No Hit
CTTCTGAGTTCAGTACGTACGAGTCCCAATGCCTGTACTCGCAGTAGTCC9165680.8645603790442No Hit
TAACGTGTCGTCAGTACGTACGAGTCAGTTGCCTTGTACTCGCAGTAGTC9123780.860608126741921No Hit
GTCGTTGCGGTCAGTACGTACGAGTCACGTTCAATGTACTCGCAGTAGTC7297390.688332372986331No Hit
CTGATGCACTCAGTACGTACGAGTCTCCATCATTGTACTCGCAGTAGTCC6635570.6259056517764445No Hit
ACAATTCGGCAGTACGTACGAGTCAGACTTCGTGTACTCGCAGTAGTCCG5756640.542999849484272No Hit
ACAGGAACTCAGTACGTACGAGTCGTCTGACGTGTACTCGCAGTAGTCCG5643790.5323551795006879No Hit
TCGTCCGATAGTACGTACGAGTCGACGGTGCTGTACTCGCAGTAGTCCGA5513290.5200456586069552No Hit
GCTACCTTAAGTACGTACGAGTCGCCTGGAATGTACTCGCAGTAGTCCGA5405140.5098443200272066No Hit
GTCTCCAGGTCAGTACGTACGAGTCACGACATCTGTACTCGCAGTAGTCC5363210.5058892379500095No Hit
GTATCCTGCCAGTACGTACGAGTCATATCAGCCGTACTCGCAGTAGTCCG5202630.49074239607172904No Hit
ATCTCGAACCAGTACGTACGAGTCGTAGAAGTTGTACTCGCAGTAGTCCG5156130.4863562449486749No Hit
AGTCATACTAGTACGTACGAGTCACTCCGGTTGTACTCGCAGTAGTCCGA4324760.40793657915999626No Hit
CTGCGTCACCAGTACGTACGAGTCTGCTCACACGTACTCGCAGTAGTCCG4137980.3903184005199089No Hit
GGAACCTAGAGTACGTACGAGTCTTGAGCTCTGTACTCGCAGTAGTCCGA3800550.35849003549943204No Hit
ACCATCTAACAGTACGTACGAGTCCCACAAGGTGTACTCGCAGTAGTCCG3653800.34464771985839543No Hit
CTGTCTAGTAGTACGTACGAGTCTGGATAGGTGTACTCGCAGTAGTCCGA3614810.3409699557231721No Hit
TAACACGAGCAGTACGTACGAGTCACTCGAGTCGTACTCGCAGTAGTCCG3573020.3370280792622596No Hit
CTTAGAAGGAGTACGTACGAGTCATCTCTGCTGTACTCGCAGTAGTCCGA3361280.3170555278903135No Hit
TGCAGTCGGCAGTACGTACGAGTCCGCACTCTTGTACTCGCAGTAGTCCG3114510.2937787426723332No Hit
TACTGATGAGTCAGTACGTACGAGTCAGTTCAACCGTACTCGCAGTAGTC3039340.286688269985882No Hit
GAACTCGTATCAGTACGTACGAGTCTGATACCGTGTACTCGCAGTAGTCC3010900.2840056433635237No Hit
AGATGAAGAGTCAGTACGTACGAGTCAGCAACATTGTACTCGCAGTAGTC2842860.2681551307889425No Hit
CTTCCGGTACAGTACGTACGAGTCTCATCTGTCGTACTCGCAGTAGTCCG2760580.26039400144690167No Hit
CCGTCAGGTAGTACGTACGAGTCAGACATATCGTACTCGCAGTAGTCCGA2751430.25953092009688133No Hit
TATACGTGGCAGTACGTACGAGTCACGTGTAGTGTACTCGCAGTAGTCCG2580930.24344836597901595No Hit
TGGAGAACCCAGTACGTACGAGTCTGAGATCATGTACTCGCAGTAGTCCG2438110.2299767508522504No Hit
GCTCTGTGTCAGTACGTACGAGTCCTGCATGTTGTACTCGCAGTAGTCCG2366020.22317680172405735No Hit
TGCAGATGCGTCAGTACGTACGAGTCTCACACTGCGTACTCGCAGTAGTC2118310.19981135022531No Hit
CTCGTCTTGGTCAGTACGTACGAGTCGTACGCGATGTACTCGCAGTAGTC1988030.18752258101431No Hit
CGATTCGAGAGTACGTACGAGTCGAACCAGTTGTACTCGCAGTAGTCCGA1921900.18128481383651274No Hit
CGCATAACTGTCAGTACGTACGAGTCCCGATCTCTGTACTCGCAGTAGTC1817330.17142116173032398No Hit
TTATCGCACCAGTACGTACGAGTCGATCGTTGAGTACTCGCAGTAGTCCG1728090.16300352460728407No Hit
GTTATCGATAGTACGTACGAGTCCACTGGTCCGTACTCGCAGTAGTCCGA1720610.16229796739437127No Hit
TTGAGACGGCAGTACGTACGAGTCCAGTATGATGTACTCGCAGTAGTCCG1679110.15838344542433366No Hit
ATACCGAAGTCAGTACGTACGAGTCGTCCTAGTTGTACTCGCAGTAGTCC1673080.15781466066579566No Hit
TCCTCGTCGGTCAGTACGTACGAGTCAATCCTCGTGTACTCGCAGTAGTC1636620.15437554088199879No Hit
TTATCGCACCAGTACGTACGAGTCTAGATCTGTGTACTCGCAGTAGTCCG1635350.15425574707713255No Hit
AGTAAGCCAGTCAGTACGTACGAGTCCCTGTTAGCGTACTCGCAGTAGTC1559430.14709453001772882No Hit
ATAGTCAGTAGTACGTACGAGTCGACAATGTTGTACTCGCAGTAGTCCGA1520360.14340921981605725No Hit
GCTAGTGGAGTCAGTACGTACGAGTCAACGTCGAGGTACTCGCAGTAGTG1501230.14160476667661584No Hit
GGTACGAGCCAGTACGTACGAGTCGACCGTAGTGTACTCGCAGTAGTCCG1483310.1399144477922044No Hit
CTGTTACCAAGTACGTACGAGTCGTCAATACAGTACTCGCAGTAGTCCGA1364510.1287085391165305No Hit
GCTATGTAAAGTACGTACGAGTCTAGTCAGACGTACTCGCAGTAGTCCGA1350540.12739080726300217No Hit
CGATACTCTCAGTACGTACGAGTCGGTTCAGACGTACTCGCAGTAGTCCG1327460.12521376709267765No Hit
CATCCTCTCTCAGTACGTACGAGTCTTAACGTTCGTACTCGCAGTAGTCC1254410.11832326516710544No Hit
GTAATCAGCAGTACGTACGAGTCATCGATCCTGTACTCGCAGTAGTCCGA1204050.11357301633792244No Hit
ATTAGCGGTGTCAGTACGTACGAGTCCCTCTGCATGTACTCGCAGTAGTC1189730.11222227044368296No Hit
TCCTTCGTAAGTACGTACGAGTCAATATCGCTGTACTCGCAGTAGTCCGA1109070.1046139489472195No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)