FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107514047
Sequences flagged as poor quality0
Sequence length140-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAATGAGTACGTACGAGTCATCAAGAGAGTACTCGCAGTAGTCCGA2920566627.16451181490731No Hit
CGCATCTACCAGTACGTACGAGTCATATAGCGTGTACTCGCAGTAGTCCG81097637.542979941960515No Hit
GTAGCCTTGGTCAGTACGTACGAGTCTAAGCCATTGTACTCGCAGTAGTC9746910.9065708409246281No Hit
GGTTAGATGGTCAGTACGTACGAGTCGATGCTCCTGTACTCGCAGTAGTC9439780.8780043411443715No Hit
ATAGACCGAAGTACGTACGAGTCTGCAGATGCGTACTCGCAGTAGTCCGA8059770.7496480901700221No Hit
TATAATCGCGTCAGTACGTACGAGTCCTTCTGAGTGTACTCGCAGTAGTC7403250.6885844414358246No Hit
TTGCCAGATCAGTACGTACGAGTCGATGCGGTTGTACTCGCAGTAGTCCG6916000.6432647819498414No Hit
TACATGCACCAGTACGTACGAGTCCCATTCTGCGTACTCGCAGTAGTCCG6656830.6191590946250958No Hit
AGACCACGCGTCAGTACGTACGAGTCCAGTATGATGTACTCGCAGTAGTC6073530.5649057187848208No Hit
TTCCTCGGTCAGTACGTACGAGTCACTGCTAGTGTACTCGCAGTAGTCCG5437900.5057850719729674No Hit
TACAGTTAGGTCAGTACGTACGAGTCATAGCTTGCGTACTCGCAGTAGTC4907010.45640640799243654No Hit
TAACACATCAGTACGTACGAGTCTGAACTTGCGTACTCGCAGTAGTCCGA4476210.41633722521858No Hit
ATTCGAAGAAGTACGTACGAGTCCAACCTGGTGTACTCGCAGTAGTCCGA4465360.4153280547610676No Hit
GGTTACGCACAGTACGTACGAGTCTGGAAGATTGTACTCGCAGTAGTCCG4444620.4133990045040347No Hit
GCTTACTAGGTCAGTACGTACGAGTCGACTCTGGTGTACTCGCAGTAGTC4312210.40108340447830043No Hit
CTATAGCTCTCAGTACGTACGAGTCTTCCTCGGTGTACTCGCAGTAGTCC4298110.3997719479390447No Hit
AGAATCAGAAGTACGTACGAGTCCACCTTCCTGTACTCGCAGTAGTCCGA3915990.3642305456141931No Hit
CAATTGTCTAGTACGTACGAGTCCGCAACCTCGTACTCGCAGTAGTCCGA3731280.3470504649499428No Hit
TACTTCCAGGTCAGTACGTACGAGTCAATGCACTTGTACTCGCAGTAGTC3503280.3258439336768711No Hit
ACAACGAGCTCAGTACGTACGAGTCACACCACATGTACTCGCAGTAGTCC3339850.3106431292647741No Hit
TACTCGACCGTCAGTACGTACGAGTCTCTCCGCGTGTACTCGCAGTAGTC3212760.2988223483020781No Hit
TCATGCAGGAGTACGTACGAGTCCTAACCGGTGTACTCGCAGTAGTCCGA2723200.25328783317030196No Hit
CTGCGATTCGTCAGTACGTACGAGTCTCGCGTCATGTACTCGCAGTAGTC1997160.18575805261985906No Hit
GCTACAATGAGTACGTACGAGTCATCAAGAGAGTACTCGCAGTAGTCCAA1920430.17862131075765383No Hit
GCTACAATGAGTACGTACGAGTCATCAAGAGAGTACTCGCAGTAGCCCGA1878000.17467484969661684No Hit
TCTACAATGAGTACGTACGAGTCATCAAGAGAGTACTCGCAGTAGTCCGA1863780.1733522318251121No Hit
CTGACAGCAAGTACGTACGAGTCCTGGTCGGTGTACTCGCAGTAGTCCGA1823190.16957691119189292No Hit
CGATCCAGAAGTACGTACGAGTCGTAGACTCTGTACTCGCAGTAGTCCGA1823060.16956481974862317No Hit
GCATGTGGTGTCAGTACGTACGAGTCCAACTAATCGTACTCGCAGTAGTC1797660.16720233775592133No Hit
TGACAAGTTTCAGTACGTACGAGTCGTATGTATCGTACTCGCAGTAGTCC1616640.1503654680583273No Hit
ATGGAGAGTGTCAGTACGTACGAGTCGCGCTCTATGTACTCGCAGTAGTC1526880.14201679153608643No Hit
ACTCACGAACAGTACGTACGAGTCAACGAGAATGTACTCGCAGTAGTCCG1397920.13002207981251046No Hit
GCCTTCCATAGTACGTACGAGTCCCGATCTCTGTACTCGCAGTAGTCCGA1304240.12130879977013609No Hit
CAGTATGATGTCAGTACGTACGAGTCGTCGTTGCGGTACTCGCAGTAGTC1252270.11647501279530478No Hit
CGCTTATGACAGTACGTACGAGTCTACCTCACTGTACTCGCAGTAGTCCG1241310.11545561111656415No Hit
TGAAGACCACAGTACGTACGAGTCTTAGCGTCTGTACTCGCAGTAGTCCG1228960.11430692400593942No Hit
GATGTAAGGAGTACGTACGAGTCGCACGCATTGTACTCGCAGTAGTCCGA1160110.10790310962808423No Hit
TAACATTGGCAGTACGTACGAGTCGCCTGGAATGTACTCGCAGTAGTCCG1157820.10769011420433275No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)