FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118763611
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGCCTCCCCATCCTTGTAGAGGGGAACATCTGAGGCCACAGATTTTG35209772.9646934531150286No Hit
GTGAAAGAATTTTCCCATTCAACCTCTCTTCCCCAGACTGTTTTCGTGAG16885771.4217966141160865No Hit
CATTCTGGAAAATGTGGAGGCTTGCACTTTCAAGAAAAGTTAAAACTCCA16866611.4201833253453366No Hit
TTTGTTGCGTTTTCTCCAGAAGGTCCAGTATTCACTCTGTGCGCTTTTGT16437511.384052729754066No Hit
AAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG15860741.3354881909072298No Hit
GATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG15848721.3344760963861229No Hit
CAGAAAGGACAAGGGTGGTTGGGAGTAGATGGAGCCTGGTTTTTTAAATG15792661.3297557953167995No Hit
AGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT15642791.3171366101355744No Hit
TCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAAGCATTGG15193371.2792950527582054No Hit
GGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC14267491.2013351463353534No Hit
TTTTAAATCCCACCCCACTTTACATATAATTGACCTTTCTGATTCTCTTC14104751.187632295889016No Hit
GGTTTTCTCCCCTCTAAATCTGGTATGGTGGAAATGGGTGCAAAGCCTTG13689071.1526316760442725No Hit
TCTGTGGGGAGTAGAGTATATGTAGAGTGGTTGTTAGGACTGAAAATGAT13601991.1452994638231402No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA13555461.1413815970954266No Hit
GCTTCATGTCTTAGCACCTCACCTAACTCTGGCTTGAATAACCTTCCCAT13493751.136185561080658No Hit
TGTCCTCACAGAGTTCAAGCTGAAGAAGATGTGGAAAAGTCCCAATGGAA13197391.1112317896767219No Hit
TCTTCACTGATTGTTAGTTTGCTGCGGATTAAAACACAGGTGATCATATT12882331.0847034619046738No Hit
CCAAATTTTATGACAAAAGTTGTGGACAGGTTTTGAAAGATATTTGTGTT12564121.0579099013754305No Hit
AGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA12079811.0171305754588416No Hit
TGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCTTT11029200.9286682938598085No Hit
TAGGCTAGAAATGTATGGTCAGAAAACACTGTGAAAAGCAAAGCTTACCA10675490.898885602257412No Hit
GAATACTGTTGCTATGGTGATCTTCTCAACTATCTAAGAAGTAAAAGAGA10390230.8748664605693068No Hit
TCTGCATTATAAAAAGGACAGCCAGATATCAACTGTTACAGAAATGAAAT10339120.870562953832719No Hit
TGTGTTCACAGAGACCTGGCCGCCAGGAACGTGCTTGTCACCCACGGGAA10294910.8668404331357018No Hit
TGCTCCCCACAATAGGACATCGGCAGAAACTAGATGATCAGACCAAGCCC10068200.8477512526964173No Hit
AAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT10018880.8435984655266167No Hit
CTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCCACCTGTCCCA9991600.8413014656484299No Hit
GAACAAACAGGTCTGAGGATTTACAGTGATAGCTTTTGTTTTCATTCTGT9697110.816505149881305No Hit
AAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG9497950.799735703556538No Hit
CTATGAATGTGCCGTTATGCAGGCAGCTGTGTAATTGGATGGGTTTTTTT9342660.7866601496311862No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG9207920.7753149236932514No Hit
TTTGACTGCTAAAATGTGTGATATCCCTAGACAGGATTTACATTATGAAA9032370.7605334600343198No Hit
CTTGAGTGTCAGGTGGCCTTGCTAATTCCTGGAGAGGTCAAGGTGACTTT8938430.7526236298086288No Hit
TTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAATGACT8880380.7477357689974583No Hit
TGGTCCTTTCGGCAAGAGACTTGATGAAACGCAGGTAAGTGGGCTCTGAA8840770.7444005723268216No Hit
AGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC8818390.7425161567376054No Hit
GATGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTAT8709010.733306264997281No Hit
GGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT8521090.7174832365108872No Hit
TATCTGAAGTGTGTGAAGAGTTTGGATAAGGACTAACTGGATTGGGCCGT8467780.7129944878486392No Hit
CGCTGTTTCATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTG8310390.6997421120851571No Hit
AGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA8293310.6983039611350315No Hit
CTAGACCTTCTCTGTCCATTTAGGTGTGAAACCATTCCAGTGTAAAACTT8251060.6947464741536025No Hit
CATCGTTTTCACCACTCACAGTGCTGGCTTCATTAGACCCACAGCTCTCC8148430.6861049383215537No Hit
GACTCCTCTTTGCATCGGGTAATTATTTATCACTGTATCTGGTCCCCTCC8005470.6740675811886522No Hit
CAGAACTGCCTATTCCTAACTGACTCATCATTTCATCTCTGAAGCAATTT7952340.669593988683958No Hit
TCAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCA7874010.6629985341217016No Hit
CAGAGGTGCTAGGAATCAAAATCTCCAAGAGTGACTATTACTGAGCGGAA7869570.6626246822353692No Hit
GTGACGGACTTCAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGGTGAGT7324320.6167141549779924No Hit
ACTCCACAGGTAGTAGGGAAGAATGTACTCGTGTTAATTGTGTATGATCG7300080.614673125760718No Hit
CCTTAGGCATTTTGGGATCTGTTCAGTCCTAGTAGGAGAGGTTGCCTTTA7259670.6112705683898412No Hit
GTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCTC7100960.5979070474709631No Hit
GCTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTTGTGTTTGT7091830.5971382934794733No Hit
TCAGTGCTTCAGAGTCTCCGTTGATTTTCAGCTTTTCAATGTCCACCTGT7089650.5969547355713191No Hit
GGCTTCACAAAGTATTGGTATTATAGGAGTGAACCACTATGCCCAGCCAG6920920.582747521881934No Hit
CAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAG6561440.5524789912290559No Hit
GTTCTGAGATGCTTTGATTCCCATGTGAGATTTAGCCCTCCTTGACTGAG6503060.5475633441290363No Hit
TCCTTGTTTTGGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGG6427280.5411826017987951No Hit
GGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTG6401020.5389714868134146No Hit
CTTAAAGCCTCCCTTCCTCTTACTCTCTGCCTGCAGGATGTGCGACGTGT6342200.534018791328263No Hit
AAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG5827760.4907024930388821No Hit
CAGCTGTATAGGTACTTGAAGTGCAGTTTCTACTAAATGCATGTTGCTTT5803560.4886648318566198No Hit
TTGGTCCACTGTACCTTGGGACTGCCCTTGATTCATTTCAACTTGGTACA5784800.48708522343599No Hit
GTTAATCTGCCCTGTGCCTTTGCGTTAATTACCTTTCTTCCATGATTTCT5704330.48030957900059135No Hit
TCAATTGTGTTACGAAAGGACTGACGATCTTCAAGCCGGGGCTTCTTTTC5688510.47897752115334385No Hit
GGGCTGTTTCACTACCTCATCATTCGATGGGATGGGTATCCAATGCAAAG5608900.47227428947070327No Hit
GGCAGAAATGGGCTTGAATAGTTAGATGCTTATTTAACCTTGGCAATAGC5594320.4710466407088279No Hit
GTTAATCACAAGGAAGCAGGACACCAATGAAACTTCAAGAAGATGCTCTG5431870.4573682085163275No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC5397240.45445233220468517No Hit
CTGCCAGTAATTTAAACATTCCCATAGAGGTATTTACCTACTACTTCCAA5355440.45093273561714114No Hit
AAATCATGACTGATATGGTAGACAGAGCCTAAACATCCCCTTAAATTGGA5327110.44854732481988946No Hit
GGCAAACCCTGAAGAACTGTAACCAGTTGCATTTACAAAATCATAATAAC5270660.4437941854091991No Hit
TCTATGGCAGTGGTGACCTCTCGCCCCTCGGACCTGCAGAGCACGGGCTT5130290.43197490854332476No Hit
GTCATGTCTTCAGGGCTTAGGCTCTGTGAGGCCAGGTGTGGAGCCTCCCT5119980.43110679751898084No Hit
TTTTGCATACGGTGATGAGTGAAGAACCTCCAAACCTCTCTCTGAAGGTC5041420.4244919767554053No Hit
TTCAACAGATATGTTCAGGCCACCAACCTCATTCTGTTTTGTTCTCTATC5013790.42216550657086366No Hit
CTCATCCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAG5012460.4220535194067146No Hit
GCCAGTGAAAATTAAGTGCTATGGTAAGATAACCTCTTTTGTTGCTGGTG4920180.4142834626340218No Hit
AGCAGGAACAATGTCTTTTCAAGTCCTTTGCCCATTTTTAAATTGAATTT4864490.4095943158885595No Hit
TCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTA4825720.40632984795317484No Hit
GAGATGGCTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTG4785040.402904556345967No Hit
CCATGATGCTCTCCTGCTTATGGTGCACCTCCTCCAGGAAGTCCAGCACG4519420.3805391198487557No Hit
TTGATTTATCTTCATTAAAGTTAAGGCGACATAAATCTAAATTACTAAAG4490580.37811076660510096No Hit
CGTCAAATGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCA4487770.37787416214550773No Hit
TAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG4318730.3636408461847796No Hit
CCTGATGTCTGCATTTGTCCTTTGACTGGTGTTTAGGTGGTGGCCCTAGG4171020.3512035348942026No Hit
ACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA4050860.34108595771814315No Hit
TGTGACTGGCCCTGACATTTCAACTTTTACTTGCTTCTGTGATTTGAGAG4041130.34026668320147324No Hit
GGTCCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCA4013660.33795368515697966No Hit
CAGCAGACTTTGTGGTCACTAAAATGTTACTGACCTTTCAGAGGTAGGAT3929600.33087575957925364No Hit
AAAAGGTGACATGGAAAGCCCCTGTTTCATACTGACCAAAACTCAGCCTG3741320.3150224187777517No Hit
CCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTGTCTCCT3718320.3130857986458495No Hit
TTGGGTCTTGTTTCCTGCAAAAAGTTCAGGATGTGTAGTCTGTTCTTTAT3565420.300211484812465No Hit
GGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGG3310300.27873015750590474No Hit
CACCCTGGACTCTTTTCTGGCTGCCGCTCTGGCACACCCCTTTTTTGTGG3301410.2779816117244869No Hit
CAGTGTGAGAACAGACTCAACAGCTGCTAAGCTGTCCTCAGCCCAACTTA3205090.26987138341558176No Hit
TGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA3145960.26489258565908713No Hit
CAGCAGTAGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACA3142020.2645608342104047No Hit
CTCCTGTTAAATTATAGTTTACACTGACACCTAGCTGTGATCCTGAAACT3058450.2575241670615758No Hit
ACGTGAGGAATGTGATTTTCTCAATAAGGCATTAAGAGTGGTCTTAGGAA2944740.24794968553120197No Hit
GGTCTCCTCTCTTTCGTGTCAAAGGACTTCTTTGCCAAGTTCACAGATAA2882710.2427267052363371No Hit
TGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTTCCTCCTC2848140.2398158809772128No Hit
TCTTTTAGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGA2844880.2395413861237345No Hit
TGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT2843770.23944792315215138No Hit
TAGGACTCTGGGAAGGTGGTGCCTCAGGTTTACCCTCTATTTTCACTTCC2830080.23829521316929306No Hit
GAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAGATCTGGAGGATCAC2822110.2376241321931513No Hit
TAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT2766620.23295182562274902No Hit
AGGTTGTCCTTTTATTCTTAAGAGTCAATTTTATTTTTTCTGTTTTAGTG2595170.21851558555254774No Hit
TTGACCCATGAGTTGGAGCCACGGCCACTCCCCCAATGTCAGGATCCAGA2555280.21515681263682696No Hit
TTCTTTGTTTACATTTTAGGCTGCTGGTCTGGCTACTATGATCTCTACCA2431860.2047647406072892No Hit
TGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA2384300.2007601469780167No Hit
GCTGCTGGAACCTGAACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTC2373360.19983898940223363No Hit
TTCCTGGAATGGTAGCCATCTGGTGATGGGACTATGAAGGTCGGGCACAA2332560.19640359368998977No Hit
TAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG2246070.18912105998528456No Hit
CAGTGTGTCTCTTTGCAGTTATGATGGTTAACGGTGATCACAGGATAGGT2191110.18449337987879133No Hit
ATCATCTCCCACTAGAGACAGAATGGGACCATTGTCTGCAGGAACGGTGG2106160.17734051552204824No Hit
TGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC2063440.17374345412922818No Hit
GCCTGGCTTCTCTCATAATTTTGAATTTAAATAAATAATGCAGATTGACT1879460.1582521770915167No Hit
AGCCCAGAGAGAGAAGGTTCACTAACTGTGCGTTTTATTCCTCCATTTTG1857960.15644185827256465No Hit
CACGTAATCAGCAATGAGTATTCTCTTCATTTCAGGTCAGTTGATTTATT1827450.15387288956715875No Hit
GCCTCGTGACCACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGG1719510.14478424708726648No Hit
GCTCTTTATCTAAATAGAGCCATTCCTTTATGTTTTAGGCAAGATAAACA1685950.1419584657121953No Hit
GGATCAGTGCATAACAGCCTAATCTCGTCGCCCACGCGGACTATTAAGTC1667240.1403830673353305No Hit
TCTCCACAGGTAGTAGGGAAGAATGTACTCGTGTTAATTGTGTATGATCG1506290.12683093645578022No Hit
TTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGTAATGGG1488710.12535068506800454No Hit
CTGAAAGAATTTTCCCATTCAACCTCTCTTCCCCAGACTGTTTTCGTGAG1430570.12045524617805702No Hit
TGGAGTTTCCTGTCAGCCTGTCTCTTATACACATCTGACGCTGCCGACGA1417460.11935137270287276No Hit
TAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG1403350.11816329835238842No Hit
GCACTCGCTAAAATTGGAAACATGCATTTTAGCAAAAAGCACAACTGGCA1348240.11352298811460018No Hit
ACCATTAGGCATTAGCACTGCCTTGTTTTTAAGTAATACAATGTTCTTGT1343260.11310366775560571No Hit
CATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG1303810.10978194322501696No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)