FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121008489
Sequences flagged as poor quality0
Sequence length138-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCATTGTCAGTACGTACGAGTCGCGGTAAGTGTACTCGCAGTAGTC42734923.53156380623842No Hit
GCTGGTTCGCAGTACGTACGAGTCTCACTACGTGTACTCGCAGTAGTCCG7375600.609510957532905No Hit
TAATGTAGCAGTACGTACGAGTCCCGACTGTTGTACTCGCAGTAGTCCGA7289990.6024362472619585No Hit
AGCCGTTCTTCAGTACGTACGAGTCCTTCCTGATGTACTCGCAGTAGTCC6748160.55766004978378No Hit
CAGATCATTCAGTACGTACGAGTCGCAACACCTGTACTCGCAGTAGTCCG6121530.5058760794872829No Hit
ACCATTCACTCAGTACGTACGAGTCAACCATGGTGTACTCGCAGTAGTCC5653400.46719036380993073No Hit
CACTTGGATTCAGTACGTACGAGTCCACTAGACTGTACTCGCAGTAGTCC5595750.46242623523709975No Hit
GGTAAGTGACAGTACGTACGAGTCTCAAGGAGTGTACTCGCAGTAGTCCG5442070.4497262997805055No Hit
CTGGTGACATCAGTACGTACGAGTCTCTCCGCGTGTACTCGCAGTAGTCC5420010.44790328718177774No Hit
CTCCACGTCCAGTACGTACGAGTCCCTGTATCTGTACTCGCAGTAGTCCG5369090.4436953179375705No Hit
TGTCGTACACAGTACGTACGAGTCTGGAAGATTGTACTCGCAGTAGTCCG5316450.43934520990506704No Hit
CCGTTAGACTCAGTACGTACGAGTCCAATCAAGTGTACTCGCAGTAGTCC5293690.43746435012505613No Hit
TATCGGAACCAGTACGTACGAGTCAGACCAGCTGTACTCGCAGTAGTCCG5278600.4362173301742492No Hit
GTGAGGACGCAGTACGTACGAGTCATTAGCGGTGTACTCGCAGTAGTCCG5261490.4348033797860248No Hit
GTGAACCACCAGTACGTACGAGTCAGTGTCTCTGTACTCGCAGTAGTCCG4857080.40138341038206005No Hit
CTTCTCATCAGTACGTACGAGTCCCACGCCATGTACTCGCAGTAGTCCGA4807720.39730435771328404No Hit
GAACTGCAAAGTACGTACGAGTCCCTCCTTGTGTACTCGCAGTAGTCCGA4580360.37851559323247147No Hit
AGGATGCGTGTCAGTACGTACGAGTCCATGCCAGTGTACTCGCAGTAGTC4423360.36554129685893355No Hit
CATCTCGATTCAGTACGTACGAGTCTACCTCACTGTACTCGCAGTAGTCC4365950.3607970016053998No Hit
CTCTCAGTCGTCAGTACGTACGAGTCTGAACTTGCGTACTCGCAGTAGTC4353040.3597301343048751No Hit
GATCTGAATAGTACGTACGAGTCTCATCATCTGTACTCGCAGTAGTCCGA4261250.3521447160620277No Hit
TGTGTAGAGAGTACGTACGAGTCATATAGCGTGTACTCGCAGTAGTCCGA4222180.3489160169581161No Hit
CAGTCCTAAAGTACGTACGAGTCATACCGCTTGTACTCGCAGTAGTCCGA4007290.33115775869245007No Hit
CTTGCACAAGTCAGTACGTACGAGTCGCTTGTCCTGTACTCGCAGTAGTC3996500.33026608571238336No Hit
GTTAGATTGAGTACGTACGAGTCTCCACCGGTGTACTCGCAGTAGTCCGA3877680.32044693988369694No Hit
CTCCGCATTGTCAGTACGTACGAGTCCAGCGACGTGTACTCGCAGTAGTC3872440.32001391241237626No Hit
TTGGTAACTAGTACGTACGAGTCTAAGCCATTGTACTCGCAGTAGTCCGA3792420.3134011532033922No Hit
CGATAGGTGCAGTACGTACGAGTCTGCATACATGTACTCGCAGTAGTCCG3681810.3042604721723284No Hit
AAGCAACTAAGTACGTACGAGTCCCAGGAGTTGTACTCGCAGTAGTCCGA3566130.29470081227111267No Hit
GGAATTCACGTCAGTACGTACGAGTCTAAGCCATTGTACTCGCAGTAGTC3564870.29459668734480277No Hit
GATTCCTCTTCAGTACGTACGAGTCCCTGTCATTGTACTCGCAGTAGTCC3539650.2925125360419962No Hit
CACTACAGGTCAGTACGTACGAGTCCGAATGAGTGTACTCGCAGTAGTCC3410230.28181741861101994No Hit
CCATCACGACAGTACGTACGAGTCAACTTAGCTGTACTCGCAGTAGTCCG3382800.279550635493019No Hit
CACTTGGATTCAGTACGTACGAGTCAACGTTCTTGTACTCGCAGTAGTCC3373450.2787779624287351No Hit
ACTCATTCATCAGTACGTACGAGTCTACGACCATGTACTCGCAGTAGTCC3338410.27588229781135437No Hit
TGAACGTCAAGTACGTACGAGTCTCACATACTGTACTCGCAGTAGTCCGA3334630.2755699230324246No Hit
AATGAGTCGGTCAGTACGTACGAGTCTAGATCTGTGTACTCGCAGTAGTC3323540.27465345840323646No Hit
ACCGGTTACTCAGTACGTACGAGTCCCGTCCACTGTACTCGCAGTAGTCC3251820.2687266014866114No Hit
GATGTCTAGAGTACGTACGAGTCTATACCACTGTACTCGCAGTAGTCCGA3239260.2676886577767284No Hit
CCAACGGAATCAGTACGTACGAGTCACCGACGGTGTACTCGCAGTAGTCC3070950.2537797162313133No Hit
GCCTTAGAGAGTACGTACGAGTCCTCAGTGATGTACTCGCAGTAGTCCGA3039290.2511633708607005No Hit
TAGCTGTTGAGTACGTACGAGTCTTGGTAACTGTACTCGCAGTAGTCCGA3010260.24876436561405207No Hit
TTCCTTGACAGTACGTACGAGTCGATGCGGTTGTACTCGCAGTAGTCCGA2677190.22123985037115868No Hit
GTCGTTGCGGTCAGTACGTACGAGTCTGAGTGACTGTACTCGCAGTAGTC2611280.2157931250591849No Hit
AGGATTACTAGTACGTACGAGTCTCCGGAGCTGTACTCGCAGTAGTCCGA2486350.20546905597672574No Hit
GGTAACTACGTCAGTACGTACGAGTCTCAGAGCGTGTACTCGCAGTAGTC2480300.20496909105277727No Hit
CCAAGCGAGCAGTACGTACGAGTCAGCAACATTGTACTCGCAGTAGTCCG2461160.20338738383883134No Hit
GCATGTAAGTCAGTACGTACGAGTCTCCTTAGGTGTACTCGCAGTAGTCC2305390.19051473322669124No Hit
GAGCATACATCAGTACGTACGAGTCAATGTGCGTGTACTCGCAGTAGTCC2233320.1845589527194245No Hit
CACTAGACTAGTACGTACGAGTCCCGAGTACTGTACTCGCAGTAGTCCGA2052880.16964760216120045No Hit
ACTATCCTGTCAGTACGTACGAGTCAACGGAGATGTACTCGCAGTAGTCC1955050.16156304538270863No Hit
CCGTACGGACAGTACGTACGAGTCTTACAGTGTGTACTCGCAGTAGTCCG1935860.15997720622724246No Hit
CAGGTATCGCAGTACGTACGAGTCATCCGTCTCGTACTCGCAGTAGTCCG1884170.15570560508362352No Hit
TAGCACTATAGTACGTACGAGTCAATGCGTGCGTACTCGCAGTAGTCCGA1877190.15512878604739871No Hit
GTAGACTCTAGTACGTACGAGTCACGGACCATGTACTCGCAGTAGTCCGA1836680.15178108702770432No Hit
ACTCACGAACAGTACGTACGAGTCCAATCAAGTGTACTCGCAGTAGTCCG1759340.1453897998842048No Hit
CAAGCTGCAGTCAGTACGTACGAGTCTCGGATGTTGTACTCGCAGTAGTC1644970.1359383968508193No Hit
GACTAGCATTCAGTACGTACGAGTCTGCCGAACTGTACTCGCAGTAGTCC1640390.13555991100756576No Hit
CAGCTTCGACAGTACGTACGAGTCGTCTGACGTGTACTCGCAGTAGTCCG1610520.13309148914337737No Hit
AAGTTCTCTAGTACGTACGAGTCCGAAGAGGTGTACTCGCAGTAGTCCGA1568020.12957933885117762No Hit
ATCACTTCCCAGTACGTACGAGTCCCGTCCACTGTACTCGCAGTAGTCCG1549020.12800920107348832No Hit
CAACCTGGTTCAGTACGTACGAGTCAGATCGAGTGTACTCGCAGTAGTCC1536400.126966299033781No Hit
GCCACGAGTAGTACGTACGAGTCAGATCTGACGTACTCGCAGTAGTCCGA1517420.1253978140326998No Hit
TATCATGCTCAGTACGTACGAGTCTATGCCTTCGTACTCGCAGTAGTCCG1501130.12405162748540724No Hit
TCCGATCTGGTCAGTACGTACGAGTCGGTGGAGCTGTACTCGCAGTAGTC1489540.12309384344101677No Hit
CTCTCAGTCGTCAGTACGTACGAGTCACCATGTCTGTACTCGCAGTAGTC1422240.11753225015478047No Hit
ATAAGTTCGTCAGTACGTACGAGTCCTCTCAGTCGTACTCGCAGTAGTCC1421900.11750415295244286No Hit
GCACTGGATTCAGTACGTACGAGTCGGTGGAGCTGTACTCGCAGTAGTCC1412890.1167595770904965No Hit
GAACCAGTTCAGTACGTACGAGTCGCTTACGTTGTACTCGCAGTAGTCCG1401990.11585881383908529No Hit
CGCTGTAGTAGTACGTACGAGTCGCTCTGTGTGTACTCGCAGTAGTCCGA1401680.11583319580165984No Hit
GGAAGAAGCAGTACGTACGAGTCGAGGTGAGCGTACTCGCAGTAGTCCGA1384790.11443742595612445No Hit
GTTAGAGACAGTACGTACGAGTCCTCCAGCGTGTACTCGCAGTAGTCCGA1356490.11209874705567144No Hit
TACTTCCAGGTCAGTACGTACGAGTCAAGAGAACTGTACTCGCAGTAGTC1333730.11021788727566047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)