FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104084064
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGCCTCCCCATCCTTGTAGAGGGGAACATCTGAGGCCACAGATTTTG23197132.228691800504638No Hit
AGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA16529311.588073079083461No Hit
TGGTCCTTTCGGCAAGAGACTTGATGAAACGCAGGTAAGTGGGCTCTGAA14029391.3478902975963736No Hit
GGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT13878591.3334020085918243No Hit
GATGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTAT13276631.275567987045548No Hit
CAGAAAGGACAAGGGTGGTTGGGAGTAGATGGAGCCTGGTTTTTTAAATG12813981.231118339114814No Hit
CATTCTGGAAAATGTGGAGGCTTGCACTTTCAAGAAAAGTTAAAACTCCA12534871.2043025145520836No Hit
CCAAATTTTATGACAAAAGTTGTGGACAGGTTTTGAAAGATATTTGTGTT12022831.1551076637437985No Hit
GGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC11729991.1269727131331075No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG11608891.115337886883433No Hit
GATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG11565381.111157611985635No Hit
TGTCCTCACAGAGTTCAAGCTGAAGAAGATGTGGAAAAGTCCCAATGGAA11562371.1108684226626662No Hit
GTGAAAGAATTTTCCCATTCAACCTCTCTTCCCCAGACTGTTTTCGTGAG11337241.0892387906759675No Hit
TATCTGAAGTGTGTGAAGAGTTTGGATAAGGACTAACTGGATTGGGCCGT11173891.0735447455241565No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA10955291.052542491038782No Hit
AGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT10872631.0446008334186492No Hit
TCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAAGCATTGG10661101.0242778375755965No Hit
TTTGTTGCGTTTTCTCCAGAAGGTCCAGTATTCACTCTGTGCGCTTTTGT10659741.024147173961232No Hit
TTTTAAATCCCACCCCACTTTACATATAATTGACCTTTCTGATTCTCTTC10429661.0020419648487207No Hit
TCTTCACTGATTGTTAGTTTGCTGCGGATTAAAACACAGGTGATCATATT10024000.9630676988169871No Hit
GCTTCATGTCTTAGCACCTCACCTAACTCTGGCTTGAATAACCTTCCCAT9935030.9545198004566771No Hit
GCTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTTGTGTTTGT9734290.9352334666717088No Hit
TCTGTGGGGAGTAGAGTATATGTAGAGTGGTTGTTAGGACTGAAAATGAT9681960.9302057998042812No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC9675340.9295697754461241No Hit
TCTGCATTATAAAAAGGACAGCCAGATATCAACTGTTACAGAAATGAAAT9415230.9045793984370172No Hit
TGTGTTCACAGAGACCTGGCCGCCAGGAACGTGCTTGTCACCCACGGGAA9373810.9005999227701178No Hit
CTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCCACCTGTCCCA9274160.8910259307322973No Hit
GCCAGTGAAAATTAAGTGCTATGGTAAGATAACCTCTTTTGTTGCTGGTG8731790.8389170891713068No Hit
GGTTTTCTCCCCTCTAAATCTGGTATGGTGGAAATGGGTGCAAAGCCTTG8499500.8165995516854531No Hit
TTGGTCCACTGTACCTTGGGACTGCCCTTGATTCATTTCAACTTGGTACA8342260.8014925320364125No Hit
AAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG8277090.7952312469274836No Hit
TGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCTTT8247710.7924085285524592No Hit
CTTGAGTGTCAGGTGGCCTTGCTAATTCCTGGAGAGGTCAAGGTGACTTT8087730.7770382601509487No Hit
GTTAATCTGCCCTGTGCCTTTGCGTTAATTACCTTTCTTCCATGATTTCT8030790.7715676820613No Hit
GACTCCTCTTTGCATCGGGTAATTATTTATCACTGTATCTGGTCCCCTCC7975470.7662527473946444No Hit
CGCTGTTTCATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTG7931800.7620571003069211No Hit
CTCATCCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAG7879640.7570457663912893No Hit
GAACAAACAGGTCTGAGGATTTACAGTGATAGCTTTTGTTTTCATTCTGT7780680.7475380669225213No Hit
CTATGAATGTGCCGTTATGCAGGCAGCTGTGTAATTGGATGGGTTTTTTT7599950.7301742176400798No Hit
TGCTCCCCACAATAGGACATCGGCAGAAACTAGATGATCAGACCAAGCCC7528460.7233057310291036No Hit
CATCGTTTTCACCACTCACAGTGCTGGCTTCATTAGACCCACAGCTCTCC7437040.7145224460105631No Hit
GTGACGGACTTCAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGGTGAGT7278950.6993337616025447No Hit
GAATACTGTTGCTATGGTGATCTTCTCAACTATCTAAGAAGTAAAAGAGA7253750.6969126416893177No Hit
TGTGACTGGCCCTGACATTTCAACTTTTACTTGCTTCTGTGATTTGAGAG7229370.6945703042494574No Hit
AAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT7060270.6783238210222076No Hit
TTTGACTGCTAAAATGTGTGATATCCCTAGACAGGATTTACATTATGAAA6640740.6380169782763286No Hit
TTGGGTCTTGTTTCCTGCAAAAAGTTCAGGATGTGTAGTCTGTTCTTTAT6618790.6359081059709583No Hit
AGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA6539430.6282834997680337No Hit
TAGGCTAGAAATGTATGGTCAGAAAACACTGTGAAAAGCAAAGCTTACCA6422310.6170310567427498No Hit
AGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC6418460.6166611634226734No Hit
CAGAGGTGCTAGGAATCAAAATCTCCAAGAGTGACTATTACTGAGCGGAA6337230.6088568947499974No Hit
ACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA6248630.6003445445788896No Hit
GGCTTCACAAAGTATTGGTATTATAGGAGTGAACCACTATGCCCAGCCAG6198480.5955263237991937No Hit
GTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCTC6147320.5906110660705947No Hit
TCAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCA6100370.5861002890894037No Hit
TTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAATGACT6026880.5790396501043618No Hit
AAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG6023920.5787552645907446No Hit
TCAGTGCTTCAGAGTCTCCGTTGATTTTCAGCTTTTCAATGTCCACCTGT5946150.5712834195252023No Hit
ACTCCACAGGTAGTAGGGAAGAATGTACTCGTGTTAATTGTGTATGATCG5868310.5638048491265676No Hit
TCCTTGTTTTGGAGATGCAGGGCACGAAGAGAGCTGTTGAACATGCCCGG5658370.5436346144208973No Hit
CCTTAGGCATTTTGGGATCTGTTCAGTCCTAGTAGGAGAGGTTGCCTTTA5615680.5395331219964663No Hit
AAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG5475200.5260363392420957No Hit
GTTCTGAGATGCTTTGATTCCCATGTGAGATTTAGCCCTCCTTGACTGAG5443300.5229715088757487No Hit
CTAGACCTTCTCTGTCCATTTAGGTGTGAAACCATTCCAGTGTAAAACTT5387910.5176498488760009No Hit
CTTAAAGCCTCCCTTCCTCTTACTCTCTGCCTGCAGGATGTGCGACGTGT5244310.5038533084180878No Hit
GTCATGTCTTCAGGGCTTAGGCTCTGTGAGGCCAGGTGTGGAGCCTCCCT5120260.4919350574166666No Hit
GGGCTGTTTCACTACCTCATCATTCGATGGGATGGGTATCCAATGCAAAG5055820.48574390792427163No Hit
TAGGACTCTGGGAAGGTGGTGCCTCAGGTTTACCCTCTATTTTCACTTCC5044650.4846707369151151No Hit
TCTATGGCAGTGGTGACCTCTCGCCCCTCGGACCTGCAGAGCACGGGCTT5035320.48377434609009884No Hit
CAGAACTGCCTATTCCTAACTGACTCATCATTTCATCTCTGAAGCAATTT5031930.4834486478160576No Hit
GCTGCTGGAACCTGAACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTC5011030.4814406555070717No Hit
GGCAAACCCTGAAGAACTGTAACCAGTTGCATTTACAAAATCATAATAAC4903040.47106538806939746No Hit
TGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA4872870.4681667695066173No Hit
CAGTGTGAGAACAGACTCAACAGCTGCTAAGCTGTCCTCAGCCCAACTTA4723590.4538245163063579No Hit
CAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAG4668120.44849517021164736No Hit
AGCAGGAACAATGTCTTTTCAAGTCCTTTGCCCATTTTTAAATTGAATTT4583010.44031812593328407No Hit
CTGCCAGTAATTTAAACATTCCCATAGAGGTATTTACCTACTACTTCCAA4387740.4215573288913853No Hit
TCCTTGTTTTGGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGG4345790.4175269328453585No Hit
TTGACCCATGAGTTGGAGCCACGGCCACTCCCCCAATGTCAGGATCCAGA4324270.41545937330041227No Hit
GGCAGAAATGGGCTTGAATAGTTAGATGCTTATTTAACCTTGGCAATAGC4250380.4083603038405572No Hit
TCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTA4162700.39993634376151954No Hit
CAGCTGTATAGGTACTTGAAGTGCAGTTTCTACTAAATGCATGTTGCTTT4148420.3985643758106909No Hit
GGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTG4078850.39188035547881755No Hit
TTGATTTATCTTCATTAAAGTTAAGGCGACATAAATCTAAATTACTAAAG4055140.3896023890842694No Hit
TTTTGCATACGGTGATGAGTGAAGAACCTCCAAACCTCTCTCTGAAGGTC4050200.38912777272032734No Hit
CCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTGTCTCCT3981620.3825388678136165No Hit
CAGCAGACTTTGTGGTCACTAAAATGTTACTGACCTTTCAGAGGTAGGAT3921890.37680023716214617No Hit
CCTGATGTCTGCATTTGTCCTTTGACTGGTGTTTAGGTGGTGGCCCTAGG3889400.37367872184544987No Hit
AAATCATGACTGATATGGTAGACAGAGCCTAAACATCCCCTTAAATTGGA3623410.34812341685659004No Hit
AGCCCAGAGAGAGAAGGTTCACTAACTGTGCGTTTTATTCCTCCATTTTG3560220.34205236259798616No Hit
CCATGATGCTCTCCTGCTTATGGTGCACCTCCTCCAGGAAGTCCAGCACG3434420.32996597826925744No Hit
GGTCCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCA3427570.32930785638808263No Hit
TTCAACAGATATGTTCAGGCCACCAACCTCATTCTGTTTTGTTCTCTATC3377330.32448098875155373No Hit
GTTAATCACAAGGAAGCAGGACACCAATGAAACTTCAAGAAGATGCTCTG3355890.3224211153015701No Hit
CGTCAAATGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCA3214450.30883209940764805No Hit
ACCATTAGGCATTAGCACTGCCTTGTTTTTAAGTAATACAATGTTCTTGT3193860.30685389071664226No Hit
GAGATGGCTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTG3192370.3067107371979634No Hit
TCAATTGTGTTACGAAAGGACTGACGATCTTCAAGCCGGGGCTTCTTTTC3168680.30443469232715586No Hit
CACCCTGGACTCTTTTCTGGCTGCCGCTCTGGCACACCCCTTTTTTGTGG3037150.29179779144672907No Hit
AGGTTGTCCTTTTATTCTTAAGAGTCAATTTTATTTTTTCTGTTTTAGTG2869830.27572232383239764No Hit
GAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAGATCTGGAGGATCAC2809120.2698895385176351No Hit
AAAAGGTGACATGGAAAGCCCCTGTTTCATACTGACCAAAACTCAGCCTG2736240.2628875060066832No Hit
GGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGG2677030.25719883497247No Hit
TTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGTAATGGG2646820.25429637336220845No Hit
GGTCTCCTCTCTTTCGTGTCAAAGGACTTCTTTGCCAAGTTCACAGATAA2593250.24914957202286028No Hit
TTCCTGGAATGGTAGCCATCTGGTGATGGGACTATGAAGGTCGGGCACAA2577000.24758833398357696No Hit
CAGCAGTAGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACA2572550.24716079495128093No Hit
ATCATCTCCCACTAGAGACAGAATGGGACCATTGTCTGCAGGAACGGTGG2498180.2400156089216501No Hit
ACGTGAGGAATGTGATTTTCTCAATAAGGCATTAAGAGTGGTCTTAGGAA2441820.2346007550204804No Hit
CTCCTGTTAAATTATAGTTTACACTGACACCTAGCTGTGATCCTGAAACT2417490.23226322138997185No Hit
TAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG2412560.23178956578790008No Hit
CAGTGTGTCTCTTTGCAGTTATGATGGTTAACGGTGATCACAGGATAGGT2329330.22379314474115847No Hit
TGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT2295500.22054288733383814No Hit
TTTTGGACATAGGTCATTAGTAACAAGTAAGTTGTTACAATTGCTGCCAA2280920.21914209652689967No Hit
GCCTCGTGACCACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGG2245320.21572178426853125No Hit
GCTCTTTATCTAAATAGAGCCATTCCTTTATGTTTTAGGCAAGATAAACA2228160.21407311689904807No Hit
TAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT2225440.2138117896703188No Hit
GCCTGGCTTCTCTCATAATTTTGAATTTAAATAAATAATGCAGATTGACT2185500.20997450676022797No Hit
AATAGGCGAATTGATCTAAAACAGATACATTCAAATTTTACAGGATCTGA2152360.2067905419219603No Hit
TGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA2090620.200858798134554No Hit
TCTTTTAGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGA2015900.1936799854394617No Hit
TTCTTTGTTTACATTTTAGGCTGCTGGTCTGGCTACTATGATCTCTACCA2006050.19273363499718843No Hit
TGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTTCCTCCTC1868960.17956255051685913No Hit
CACGTAATCAGCAATGAGTATTCTCTTCATTTCAGGTCAGTTGATTTATT1749980.16813140578369423No Hit
TCGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA1724150.16564975787263647No Hit
TAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG1638630.15743332235758972No Hit
AAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCAA1561970.15006812185965376No Hit
TGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC1540510.14800632688592943No Hit
TAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG1437810.13813930247765882No Hit
GCACTCGCTAAAATTGGAAACATGCATTTTAGCAAAAAGCACAACTGGCA1363130.13096433283004782No Hit
GGATCAGTGCATAACAGCCTAATCTCGTCGCCCACGCGGACTATTAAGTC1360270.13068955493513398No Hit
TGCAGGAACAATGTCTTTTCAAGTCCTTTGCCCATTTTTAAATTGAATTT1269060.12192644591587046No Hit
TCTCCACAGGTAGTAGGGAAGAATGTACTCGTGTTAATTGTGTATGATCG1268550.12187744706048372No Hit
GGTATCAGGAATGGACTTAGTCTGTTGCCCTCAACATGGTTGGTTCTATC1094520.10515730823116207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)