FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97723576
Sequences flagged as poor quality0
Sequence length138-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCACAACAGTACGTACGAGTCAACTCCATGGTACTCGCAGTAGTCCGA3166082132.398344694222No Hit
ATGGTTCGAGTCAGTACGACGAGTCTTACTTACGGTACTCGCAGTAGTCC10362491.0603879252228756No Hit
AGAAGCTGCGTCAGTACGTACGAGTCTCGAACGCTGTACTCGCAGTAGTC8269550.8462185215162409No Hit
AACAATGCATCAGTACGTCACGCGTGACGTACTCGCAGTAGTCCGACTGA8070990.8258999854855904No Hit
TATTCAACGTCAGTACGTACGAGTCTGAACGGTTGTACTCGCAGTAGTCC5140570.5260317121428303No Hit
TGTAACAGTTCAGTACGTACGAGTCCTGACGGATGTACTCGCAGTAGTCC4898120.50122193645472No Hit
TATGAGACCGTCAGTACGTACGAGTCCCGTCAGGTGTACTCGCAGTAGTC4813370.4925495153799939No Hit
GTAACCATAGTCAGTACGTACGAGTCGGTCCTGGTGTACTCGCAGTAGTC4522820.4628176930406231No Hit
AAGCACAACAGTACGTACGAGTCAACTCCATGGTACTCGCAGTAGTCCAA4377510.4479482003401104No Hit
AAGCAGCATTCAGTACGTACGAGTCAAGAGAACTGTACTCGCAGTAGTCC4108850.42045636970959804No Hit
ACTCCAACTGTCAGTACGTACGAGTCCCGATCTCTGTACTCGCAGTAGTC4008880.4102264943722485No Hit
TTCCTTGACAGTACGTACGAGTCTCACTCGCTGTACTCGCAGTAGTCCGA3709750.37961668533292314No Hit
CCACGAAGGCAGTACGTACGAGTCACTACTCATGTACTCGCAGTAGTCCG3071680.3143233317618258No Hit
ACTGTGTCAAGTACGTACGAGTCAGTACTACAGTACTCGCAGTAGTCCGA2996380.3066179240104763No Hit
GCACAGTAACAGTACGTACGAGTCAGTTCAACCGTACTCGCAGTAGTCCG2311410.23652531913076943No Hit
ACTCACGAACAGTACGTACGAGTCTGGATAGGTGTACTCGCAGTAGTCCG2300000.23535774008106294No Hit
GATCGCACTTCAGTACGTACGAGTCAAGCCACACGTACTCGCAGTAGTCC2208310.22597515260800527No Hit
CTCCACGTCCAGTACGTACGAGTCTGTCATAGCGTACTCGCAGTAGTCCG2206810.2258216584296915No Hit
TGATTATCGCAGTACGTACGAGTCCATGCCAGTGTACTCGCAGTAGTCCG2147920.2197954769890942No Hit
ATAGTGGTTTCAGTACGTACGAGTCTCAAGGAGTGTACTCGCAGTAGTCC2048270.20959834707645164No Hit
ACCGCTGTGTCAGTACGTACGAGTCACGTTCAATGTACTCGCAGTAGTCC2009290.20560954502933868No Hit
GATTAGACATCAGTACGTACGAGTCGGTGGAGCTGTACTCGCAGTAGTCC1953760.19992719054816416No Hit
CTGCTGCTTAGTACGTACGAGTCATATCAGCCGTACTCGCAGTAGTCCGA1893770.19378844671013679No Hit
CATGTGAACCAGTACGTACGAGTCTGAACGGTTGTACTCGCAGTAGTCCG1778560.1819990705211197No Hit
GAGCACATTAGTACGTACGAGTCCCTCTGCATGTACTCGCAGTAGTCCGA1732450.17728065947975544No Hit
TCACGCGACTCAGTACGTACGAGTCCATGACGTTGTACTCGCAGTAGTCC1695790.17352926176176772No Hit
TAGATAGCCGTCAGTACGTACGAGTCTGTAACAGTGTACTCGCAGTAGTC1563640.1600064246523275No Hit
CTTCCGTGGGTCAGTACGTACGAGTCACCGGTTACGTACTCGCAGTAGTC1534200.1569938455792899No Hit
TCAGCGACCAGTACGTACGAGTCTAATCACGCGTACTCGCAGTAGTCCGA1495090.15299174070338972No Hit
ATCATAGACTCAGTACGTACGAGTCATGCTCACCGTACTCGCAGTAGTCC1463580.14976733966427916No Hit
GCCAACAATCAGTACGTACGAGTCCCATGATGTGTACTCGCAGTAGTCCG1412040.14449327969741918No Hit
TGGTCGCTACAGTACGTACGAGTCAGCCACTTCGTACTCGCAGTAGTCCG1340970.13722072552891434No Hit
AAGCACAACAGTACGTACGAGTCAACTCCATGTACTCGCAGTAGTCCGAC1294800.13249617472041753No Hit
ACATGACTAAGTACGTACGAGTCGCCAACAATGTACTCGCAGTAGTCCGA1282840.131272314471996No Hit
GATGCGGTTAGTACGTACGAGTCCTTCTGAGTGTACTCGCAGTAGTCCGA1282290.13121603327328096No Hit
GACCAGAGACAGTACGTACGAGTCTCCATCATTGTACTCGCAGTAGTCCG1254780.12840095004300703No Hit
ATGGTTCGAGTCAGTACGTACGAGTCTTACTTACGGTACTCGCAGTAGTC1238380.12672274702677683No Hit
CTGCGATTCGTCAGTACGTACGAGTCCAGATCATTGTACTCGCAGTAGTC1210490.12386877860466343No Hit
TACGAGTGACAGTACGTACGAGTCCGCTGTAGTGTACTCGCAGTAGTCCG1139090.11656245571692955No Hit
AACAATGCATCAGTACGTACGAGTCACGCGTGACGTACTCGCAGTAGTCC1125700.11519226435184893No Hit
GCCGTCTTCAGTACGTACGAGTCGTGGTACGGGTACTCGCAGTAGTCCGA1108340.11341582506149796No Hit
GTTAGAGACAGTACGTACGAGTCCGACCTAGCGTACTCGCAGTAGTCCGA1101580.11272407796456405No Hit
AACGATGACGTCAGTACGTACGAGTCGAGTCCAATGTACTCGCAGTAGTC1064780.10895835412326703No Hit
AAGCTGGTCTCAGTACGTACGAGTCCAGGAGCCTGTACTCGCAGTAGTCC983650.10065636566553807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)