FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112983763
Sequences flagged as poor quality0
Sequence length139-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCAACCTCAGTACGTACGAGTCGAGTGCATAGTACTCGCAGTAGTCC1337647911.839293226585133No Hit
GACACCGATTCAGTACGTACGAGTCTTCGTGATGGTACTCGCAGTAGTCC49229004.357174756163857No Hit
AATACCTTGCAGTACGTACGAGTCTGCCTGGTTGTACTCGCAGTAGTCCG36102483.1953688779156697No Hit
AGGTCAGCAAGTACGTACGAGTCCTAGACATGGTACTCGCAGTAGTCCGA31480632.7862968239073433No Hit
TCTTAGCGAAGTACGTACGAGTCCGTTGATCCGTACTCGCAGTAGTCCGA31103512.752918576450671No Hit
CACTACAGGTCAGTACGTACGAGTCTCGACGTTGGTACTCGCAGTAGTCC24865932.2008410181912597No Hit
AATGCGTGCAGTACGTACGAGTCACGTCGCTCGTACTCGCAGTAGTCCGA19429331.7196568324600765No Hit
CTGGACACTGTCAGTACGTACGAGTCTAGCAGAGTGTACTCGCAGTAGTC16034611.4191959600425064No Hit
CCTTGACGGTCAGTACGTACGAGTCGCAACACCTGTACTCGCAGTAGTCC15576811.3786768635064846No Hit
CAACCTGGTTCAGTACGTACGAGTCGAACCAGTTGTACTCGCAGTAGTCC14642301.2959649786137855No Hit
TTACTTACGAGTACGTACGAGTCTGTCGTGAGGTACTCGCAGTAGTCCGA14530261.2860485094659133No Hit
GTGATGTGCAGTACGTACGAGTCCAAGCGCATGTACTCGCAGTAGTCCGA11515661.0192314093840191No Hit
TAGAGCCAGGTCAGTACGTACGAGTCCATACGCAGGTACTCGCAGTAGTC11344111.0040478117196363No Hit
AGACTAGTAAGTACGTACGAGTCCAGCTTACCGTACTCGCAGTAGTCCGA11148120.9867010713743001No Hit
CTTGCGACCTCAGTACGTACGAGTCTGAGAGGACGTACTCGCAGTAGTCC9880740.8745274309902388No Hit
TTGTTAGAGGTCAGTACGTACGAGTCAATCCTCGTGTACTCGCAGTAGTC9829790.8700179334618197No Hit
TGAACCAATAGTACGTACGAGTCGCAACCAACGTACTCGCAGTAGTCCGA9270780.8205409125911304No Hit
TGCGTAATCGTCAGTACGTACGAGTCAGTACGAATGTACTCGCAGTAGTC8466160.7493253698763777No Hit
CGTATCGAACAGTACGTACGAGTCATCAGTCGAGTACTCGCAGTAGTCCG7674380.6792462736437624No Hit
TGAACCAATAGTACGTACGAGTCATCCTGCCTGTACTCGCAGTAGTCCGA7570180.6700237095130209No Hit
ATAGACCGAAGTACGTACGAGTCTGCTGCGTCGTACTCGCAGTAGTCCGA7225350.6395033948373626No Hit
TGAGGTCTCTCAGTACGTACGAGTCTTACGGACCGTACTCGCAGTAGTCC7046220.6236489043120294No Hit
CGTTGATCCTCAGTACGTACGAGTCACTCATTCAGTACTCGCAGTAGTCC6109080.5407042426087366No Hit
CTTCGTGCAGTCAGTACGTACGAGTCGACGCTTGCGTACTCGCAGTAGTC5880350.520459740750536No Hit
CTTATTGAGTCAGTACGTACGAGTCACTTGATCTGTACTCGCAGTAGTCC5526980.48918356525264606No Hit
TTACTTACGAGTACGTACTCGCAGTAGTCCGACTGAGATACTAGATCGGC5354870.47395040294418234No Hit
TGATAGAGGTCAGTACGTACGAGTCCGACCTAGCGTACTCGCAGTAGTCC5149370.4557619487324032No Hit
CCGTCCACTAGTACGTACGAGTCACTGGTGCTGTACTCGCAGTAGTCCGA5070590.4487892654097563No Hit
CATACAACCGTCAGTACGTACGAGTCGCGTTCAGAGTACTCGCAGTAGTC4977100.4405146250970593No Hit
TGAGCTAGACAGTACGTACGAGTCAGCAAGTCCGTACTCGCAGTAGTCCG4966030.43953483829353424No Hit
TGAGACAGCTCAGTACGTACGAGTCAGAAGCTGCGTACTCGCAGTAGTCC4931320.4364627154434571No Hit
TTATCGCACCAGTACGTACGAGTCCCGCACTCAGTACTCGCAGTAGTCCG4898180.43352955061339216No Hit
ATGCGGATTCAGTACGTACGAGTCATCCTGCCTGTACTCGCAGTAGTCCG4816590.4263081589874113No Hit
GCTTGTCCTAGTACGTACGAGTCGCCAACAATGTACTCGCAGTAGTCCGA4592550.4064787610233871No Hit
CTAGTGCTGCAGTACGTACGAGTCTTCTGGTGCGTACTCGCAGTAGTCCG4343500.3844357706513988No Hit
CTGACAGCAAGTACGTACGAGTCCGTACATCTGTACTCGCAGTAGTCCGA4319640.3823239627803864No Hit
AGGATCATGTCAGTACGTACGAGTCTTGATCGTAGTACTCGCAGTAGTCC4051060.35855240544608163No Hit
TCCGGAGCTAGTACGTACGAGTCCGTACATCTGTACTCGCAGTAGTCCGA3962150.35068313311533095No Hit
ACGCTAGTGAGTACGTACGAGTCTTACACCTTGTACTCGCAGTAGTCCGA3887220.3440512067207392No Hit
GAATCACATGTCAGTACGTACGAGTCCATCCACCGGTACTCGCAGTAGTC3870740.34259259005207676No Hit
GTAGCTACAGTCAGTACGTACGAGTCTCAACACTCGTACTCGCAGTAGTC3729980.3301341627292056No Hit
GTCGAGTACCAGTACGTACGAGTCATAATGTGGGTACTCGCAGTAGTCCG3717240.3290065670763683No Hit
CCACGAAGGCAGTACGTACGAGTCGAATTGTTGGTACTCGCAGTAGTCCG3529220.312365237826253No Hit
GTGTACCGTGTCAGTACGTACGAGTCTCTACAGTTGTACTCGCAGTAGTC3453370.30565188380209996No Hit
TGAGGTCTCTCAGTACGTACGAGTCCGAATCCTGGTACTCGCAGTAGTCC3364070.2977480932370787No Hit
GCTACCGAGTCAGTACGTACGAGTCGTATTACTGGTACTCGCAGTAGTCC3343960.29596819146482134No Hit
TGTCGTACACAGTACGTACGAGTCTGCAGTCGGGTACTCGCAGTAGTCCG3193130.28261848563142655No Hit
CTGTCACTTTCAGTACGTACGAGTCATACTTGCTGTACTCGCAGTAGTCC3181820.28161745683758116No Hit
ACGGTGCCTGTCAGTACGTACGAGTCAGCCGTTCTGTACTCGCAGTAGTC3171540.28070759158552716No Hit
GCGTTCAGACAGTACGTACGAGTCAGTTCAACCGTACTCGCAGTAGTCCG3159110.2796074335035203No Hit
AGCAACATTCAGTACGTACGAGTCTGACCTATGGTACTCGCAGTAGTCCG3030130.2681916338722052No Hit
GTGTACCGTGTCAGTACACGAGTCTCTACAGTTGTACTCGCAGTAGTCCG2995720.26514606351002845No Hit
ACGACGATTCAGTACGTACGAGTCCTGTCACTTGTACTCGCAGTAGTCCG2941810.26037458143432524No Hit
TCCTTCGTAAGTACGTACGAGTCTAGCAGAGTGTACTCGCAGTAGTCCGA2924540.25884604321419175No Hit
TGCCGCTACAGTACGTACGAGTCGGATACCTAGTACTCGCAGTAGTCCGA2866830.25373822962508336No Hit
TACTACTGTAGTACGTACGAGTCCTGAGTCCAGTACTCGCAGTAGTCCGA2783380.24635221257412004No Hit
AGACTAGTAAGTACGTACGAGTCTGCGTCTTAGTACTCGCAGTAGTCCGA2725390.2412196166629713No Hit
ATCTACATCCAGTACGTACGAGTCTCGCTGTAAGTACTCGCAGTAGTCCG2567760.22726805443716724No Hit
AGTACGAATAGTACGTACGAGTCGCACTGGATGTACTCGCAGTAGTCCGA2540080.22481814488688961No Hit
GTGTACCGTGTCAGTACGACGAGTCTCTACAGTTGTACTCGCAGTAGTCC2530110.2239357172056661No Hit
TGCATCGGATCAGTACGTACGAGTCATGCGCCGTGTACTCGCAGTAGTCC2523580.2233577580523672No Hit
AGCCTCTAGAGTACGTACGAGTCAGCTGCACTGTACTCGCAGTAGTCCGA2457840.21753922287045796No Hit
ATATCCGATGTCAGTACGTACGAGTCCTCCAGCGTGTACTCGCAGTAGTC2387550.21131797495539248No Hit
CTACGTTGGAGTACGTACGAGTCAGTGTCTCTGTACTCGCAGTAGTCCGA2269900.2009049742837827No Hit
TGAAGACCACAGTACGTACGAGTCTGCAGCCTTGTACTCGCAGTAGTCCG2206340.19527938718061638No Hit
TTCCTCGGTCAGTACGTACGAGTCGATCGATACGTACTCGCAGTAGTCCG2134770.18894484865050917No Hit
CGTTGATCCTCAGTACGTACGAGTCCACCAAGATGTACTCGCAGTAGTCC2132650.18875721106934631No Hit
TCCAGCAGCCAGTACGTACGAGTCCCATATCTTGTACTCGCAGTAGTCCG2094460.18537707935962444No Hit
GCGGTAGTCGTCAGTACGTACGAGTCTATAGTGCGGTACTCGCAGTAGTC2066960.1829431013020871No Hit
ATTGATTGGTCAGTACGTACGAGTCTCACTCGCTGTACTCGCAGTAGTCC2065230.18278998195519475No Hit
TTGGACTTCCAGTACGTACGAGTCTGAGCAGATGTACTCGCAGTAGTCCG1891960.16745415002684944No Hit
AGTAAGCCAGTCAGTACGTACGAGTCCCGCTATCTGTACTCGCAGTAGTC1887710.16707798978159366No Hit
TATCGGAACCAGTACGTACGAGTCCTGTGCTCAGTACTCGCAGTAGTCCG1876270.16606545490965813No Hit
ATCACCGCACAGTACGTACGAGTCAGCGTCGTCGTACTCGCAGTAGTCCG1874480.1659070250651857No Hit
ACGACGATTCAGTACGTACGAGTCAGTACGAATGTACTCGCAGTAGTCCG1839750.1628331320492485No Hit
CTAGAGTCGAGTACGTACGAGTCCATACAACCGTACTCGCAGTAGTCCGA1772110.15684643111063667No Hit
CCTGTATCTCAGTACGTACGAGTCTCGTACCTTGTACTCGCAGTAGTCCG1770700.15672163441750475No Hit
ACTCGAGTCGTCAGTACGTACGAGTCTCTCGTATCGTACTCGCAGTAGTC1698460.15032779533108664No Hit
TGCCAATGGTCAGTACGTACGAGTCCAGCTTACCGTACTCGCAGTAGTCC1671430.14793541617125994No Hit
TCACGACAATCAGTACGTACGAGTCGCTGGTTCGGTACTCGCAGTAGTCC1657610.14671223156198118No Hit
CGAGCCGTTAGTACGTACGAGTCGATTAGACAGTACTCGCAGTAGTCCGA1654640.14644936193176714No Hit
ACTACCATCCAGTACGTACGAGTCTATTCAACGGTACTCGCAGTAGTCCG1650530.14608559284753156No Hit
AATACTCCGTCAGTACGTACGAGTCCGACAGGAAGTACTCGCAGTAGTCC1649240.1459714171495598No Hit
AGTAAGCCAGTCAGTACGTACGAGTCCCGCTATCCGTACTCGCAGTAGTC1637350.14491905354577364No Hit
CTGCAGAGCTCAGTACGTACGAGTCAAGTTCTCTGTACTCGCAGTAGTCC1580140.13985549410316594No Hit
GATCGATACAGTACGTACGAGTCACGTAGTATGTACTCGCAGTAGTCCGA1579300.13978114713704481No Hit
GCGGAGATTGTCAGTACGTACGAGTCATGGACGTTGTACTCGCAGTAGTC1556190.13773572048578342No Hit
ACAGCGGTCAGTACGTACGAGTCTTCCAGATTGTACTCGCAGTAGTCCGA1513560.13396261195513554No Hit
GACGTGGTCTCAGTACGTACGAGTCTCCTTCGTAGTACTCGCAGTAGTCC1472040.13028774762971915No Hit
ACATTGACACAGTACGTACGAGTCCCACACAGAGTACTCGCAGTAGTCCG1455950.1288636491953273No Hit
TCTTGGTAGTCAGTACGTACGAGTCCTGCGTCACGTACTCGCAGTAGTCC1447610.128125490031696No Hit
TACTAGTAGCAGTACGTACGAGTCCTACGGTATGTACTCGCAGTAGTCCG1446140.12799538284098397No Hit
GCCTGCATCAGTACGTACGAGTCAACTCCATGGTACTCGCAGTAGTCCGA1371580.12139620451480271No Hit
CTCTAAGAGCAGTACGTACGAGTCCGGTCGACAGTACTCGCAGTAGTCCG1344490.11899851485739593No Hit
AGGATTACTAGTACGTACGAGTCTCTCGATGCGTACTCGCAGTAGTCCGA1285100.11374200733604528No Hit
ACCTGCAGATCAGTACGTACGAGTCAGGATTACTGTACTCGCAGTAGTCC1263360.11181783704619574No Hit
GGATAGTCAGTCAGTACGTACGAGTCCACAATAACGTACTCGCAGTAGTC1247010.11037072645562354No Hit
ACCTGTACCAGTACGTACGAGTCTTCCTTGACGTACTCGCAGTAGTCCGA1188770.10521600347122445No Hit
GACCTTGTGGTCAGTACGTACGAGTCCTTGGTCATGTACTCGCAGTAGTC1174190.10392555255926464No Hit
TGGTCTACGGTCAGTACGTACGAGTCAACTTGTCGGTACTCGCAGTAGTC1132900.10027104514123858No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)