FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94290058
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA14734441.5626716445545086No Hit
GGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT12898601.3679703113556256No Hit
TGGTCCTTTCGGCAAGAGACTTGATGAAACGCAGGTAAGTGGGCTCTGAA12353291.310137066624776No Hit
GGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC12072391.2803460148470795No Hit
GCTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTTGTGTTTGT11097401.1769427483012047No Hit
CATTCTGGAAAATGTGGAGGCTTGCACTTTCAAGAAAAGTTAAAACTCCA11011301.1678113508001025No Hit
AGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT10281421.0904034018093403No Hit
TGTCCTCACAGAGTTCAAGCTGAAGAAGATGTGGAAAAGTCCCAATGGAA10071181.068106247214314No Hit
TGTGACTGGCCCTGACATTTCAACTTTTACTTGCTTCTGTGATTTGAGAG9864401.0461760454108535No Hit
GATGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTAT9776931.0368993515732061No Hit
AAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG9701121.0288592674319916No Hit
TCCTTGTTTTGGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGG9579171.0159257723650992No Hit
CGCTGTTTCATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTG9274700.983634987264511No Hit
TATCTGAAGTGTGTGAAGAGTTTGGATAAGGACTAACTGGATTGGGCCGT8931270.947212271308604No Hit
GATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG8827380.9361941425468208No Hit
TTGGTCCACTGTACCTTGGGACTGCCCTTGATTCATTTCAACTTGGTACA8751480.9281445133907967No Hit
CTTAGCCTCCCCATCCTTGTAGAGGGGAACATCTGAGGCCACAGATTTTG8725520.9253913068968522No Hit
GCCAGTGAAAATTAAGTGCTATGGTAAGATAACCTCTTTTGTTGCTGGTG8714440.9242162095180809No Hit
CTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCCACCTGTCCCA8456620.8968729237604245No Hit
CAGAAAGGACAAGGGTGGTTGGGAGTAGATGGAGCCTGGTTTTTTAAATG8451910.8963734013187264No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA8404720.8913686318869376No Hit
TTGGGTCTTGTTTCCTGCAAAAAGTTCAGGATGTGTAGTCTGTTCTTTAT8387780.8895720479883468No Hit
CCAAATTTTATGACAAAAGTTGTGGACAGGTTTTGAAAGATATTTGTGTT8205780.8702699069291059No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC7977320.8460404171137534No Hit
TCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAAGCATTGG7946790.8428025359789257No Hit
GCTTCATGTCTTAGCACCTCACCTAACTCTGGCTTGAATAACCTTCCCAT7938780.8419530296608789No Hit
TCTGTGGGGAGTAGAGTATATGTAGAGTGGTTGTTAGGACTGAAAATGAT7928930.8409083808178376No Hit
TTTTAAATCCCACCCCACTTTACATATAATTGACCTTTCTGATTCTCTTC7714960.8182156383868169No Hit
CTTAAAGCCTCCCTTCCTCTTACTCTCTGCCTGCAGGATGTGCGACGTGT7546310.8003293411909874No Hit
TGCTCCCCACAATAGGACATCGGCAGAAACTAGATGATCAGACCAAGCCC7458370.7910028011648905No Hit
TTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGTAATGGG7455280.7906750889897639No Hit
CTATGAATGTGCCGTTATGCAGGCAGCTGTGTAATTGGATGGGTTTTTTT7323820.7767330040246662No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG7061200.7488806508105023No Hit
CCTTAGGCATTTTGGGATCTGTTCAGTCCTAGTAGGAGAGGTTGCCTTTA7035370.7461412315601714No Hit
GGCTTCACAAAGTATTGGTATTATAGGAGTGAACCACTATGCCCAGCCAG6957200.7378508559195075No Hit
CTTGAGTGTCAGGTGGCCTTGCTAATTCCTGGAGAGGTCAAGGTGACTTT6818350.7231250191828285No Hit
AGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC6809030.7221365798714431No Hit
GTGACGGACTTCAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGGTGAGT6806910.721911741744819No Hit
GACTCCTCTTTGCATCGGGTAATTATTTATCACTGTATCTGGTCCCCTCC6693110.7098426007967882No Hit
GTGAAAGAATTTTCCCATTCAACCTCTCTTCCCCAGACTGTTTTCGTGAG6675340.7079579906505095No Hit
TGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCTTT6570920.6968836523570703No Hit
CCTGATGTCTGCATTTGTCCTTTGACTGGTGTTTAGGTGGTGGCCCTAGG6536810.6932660917442643No Hit
ACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA6520250.6915098090193136No Hit
TCTTCACTGATTGTTAGTTTGCTGCGGATTAAAACACAGGTGATCATATT6437440.6827273348373589No Hit
CATCGTTTTCACCACTCACAGTGCTGGCTTCATTAGACCCACAGCTCTCC6404330.6792158299446586No Hit
TAGGACTCTGGGAAGGTGGTGCCTCAGGTTTACCCTCTATTTTCACTTCC6392940.6780078552926545No Hit
AGCCCAGAGAGAGAAGGTTCACTAACTGTGCGTTTTATTCCTCCATTTTG6312610.6694883992965621No Hit
TGTGTTCACAGAGACCTGGCCGCCAGGAACGTGCTTGTCACCCACGGGAA6277220.6657350873620207No Hit
TCTGCATTATAAAAAGGACAGCCAGATATCAACTGTTACAGAAATGAAAT6227840.6604980559032003No Hit
GTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCTC6152360.6524929701496206No Hit
CTCATCCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAG5982820.634512283362897No Hit
TTTGTTGCGTTTTCTCCAGAAGGTCCAGTATTCACTCTGTGCGCTTTTGT5924920.628371657168776No Hit
GAACAAACAGGTCTGAGGATTTACAGTGATAGCTTTTGTTTTCATTCTGT5872180.6227782784904003No Hit
GGTTTTCTCCCCTCTAAATCTGGTATGGTGGAAATGGGTGCAAAGCCTTG5861110.62160424166883No Hit
GTTAATCTGCCCTGTGCCTTTGCGTTAATTACCTTTCTTCCATGATTTCT5603630.5942970148560095No Hit
GCTGCTGGAACCTGAACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTC5573630.591115343252838No Hit
GAATACTGTTGCTATGGTGATCTTCTCAACTATCTAAGAAGTAAAAGAGA5474390.5805903735895463No Hit
CAGAGGTGCTAGGAATCAAAATCTCCAAGAGTGACTATTACTGAGCGGAA5473410.5804864389838428No Hit
TCAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCA5472620.5804026549649592No Hit
TTTGACTGCTAAAATGTGTGATATCCCTAGACAGGATTTACATTATGAAA5379350.5705108379506989No Hit
GTTCTGAGATGCTTTGATTCCCATGTGAGATTTAGCCCTCCTTGACTGAG5357400.5681829148943783No Hit
TTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAATGACT5303280.5624431793222568No Hit
GGGCTGTTTCACTACCTCATCATTCGATGGGATGGGTATCCAATGCAAAG5276100.5595605848497834No Hit
TTTTGCATACGGTGATGAGTGAAGAACCTCCAAACCTCTCTCTGAAGGTC5208640.5524060659714516No Hit
CGTCAAATGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCA5148730.546052267779918No Hit
ACTCCACAGGTAGTAGGGAAGAATGTACTCGTGTTAATTGTGTATGATCG5016830.5320635182979737No Hit
TAGGCTAGAAATGTATGGTCAGAAAACACTGTGAAAAGCAAAGCTTACCA5015400.5319118586182225No Hit
AAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT5009850.5313232493716358No Hit
CAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAG4974520.5275763007803007No Hit
AGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA4842420.5135663401543353No Hit
GTCATGTCTTCAGGGCTTAGGCTCTGTGAGGCCAGGTGTGGAGCCTCCCT4838700.513171812875542No Hit
TTGATTTATCTTCATTAAAGTTAAGGCGACATAAATCTAAATTACTAAAG4835020.512781527825553No Hit
AAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG4741720.5028865291396895No Hit
CTAGACCTTCTCTGTCCATTTAGGTGTGAAACCATTCCAGTGTAAAACTT4725960.5012150909908232No Hit
AGCAGGAACAATGTCTTTTCAAGTCCTTTGCCCATTTTTAAATTGAATTT4594670.48729103549814345No Hit
CTGCCAGTAATTTAAACATTCCCATAGAGGTATTTACCTACTACTTCCAA4543370.48185037705672No Hit
CAGCAGACTTTGTGGTCACTAAAATGTTACTGACCTTTCAGAGGTAGGAT4536390.48111010813038213No Hit
TCAGTGCTTCAGAGTCTCCGTTGATTTTCAGCTTTTCAATGTCCACCTGT4478410.4749609974786525No Hit
CAGAACTGCCTATTCCTAACTGACTCATCATTTCATCTCTGAAGCAATTT4471950.4742758775267696No Hit
AAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG4339990.46028076470161894No Hit
GGTCCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCA4316900.4578319381243779No Hit
TCTATGGCAGTGGTGACCTCTCGCCCCTCGGACCTGCAGAGCACGGGCTT4175880.4428759604750694No Hit
CAGTGTGAGAACAGACTCAACAGCTGCTAAGCTGTCCTCAGCCCAACTTA4149780.44010790618031015No Hit
ACCATTAGGCATTAGCACTGCCTTGTTTTTAAGTAATACAATGTTCTTGT4137680.4388246319670309No Hit
TCAATTGTGTTACGAAAGGACTGACGATCTTCAAGCCGGGGCTTCTTTTC4115780.4365020116967157No Hit
TCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTA4071600.43181646998244505No Hit
AAATCATGACTGATATGGTAGACAGAGCCTAAACATCCCCTTAAATTGGA3941200.4179868040806593No Hit
GGCAGAAATGGGCTTGAATAGTTAGATGCTTATTTAACCTTGGCAATAGC3915080.4152166286714979No Hit
CAGTGTGTCTCTTTGCAGTTATGATGGTTAACGGTGATCACAGGATAGGT3899940.4136109450690973No Hit
AATAGGCGAATTGATCTAAAACAGATACATTCAAATTTTACAGGATCTGA3770500.3998830926586131No Hit
GTTAATCACAAGGAAGCAGGACACCAATGAAACTTCAAGAAGATGCTCTG3675480.38980567813416767No Hit
TTCAACAGATATGTTCAGGCCACCAACCTCATTCTGTTTTGTTCTCTATC3327210.35286965249294894No Hit
TGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA3320040.35210923297979096No Hit
AGGTTGTCCTTTTATTCTTAAGAGTCAATTTTATTTTTTCTGTTTTAGTG3319810.35208484016416663No Hit
GGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTG3295020.34945571886274585No Hit
TTGACCCATGAGTTGGAGCCACGGCCACTCCCCCAATGTCAGGATCCAGA3259820.34572255751502456No Hit
CAGCAGTAGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACA3228600.34241149793332404No Hit
CCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTGTCTCCT3168430.3360301252545629No Hit
GCCTCGTGACCACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGG3163140.3354690904952036No Hit
AAAAGGTGACATGGAAAGCCCCTGTTTCATACTGACCAAAACTCAGCCTG3087500.32744703582640705No Hit
CACCCTGGACTCTTTTCTGGCTGCCGCTCTGGCACACCCCTTTTTTGTGG2772050.29399175891905804No Hit
CAGCTGTATAGGTACTTGAAGTGCAGTTTCTACTAAATGCATGTTGCTTT2760410.2927572703370275No Hit
GGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGG2744750.2910964377601719No Hit
CCATGATGCTCTCCTGCTTATGGTGCACCTCCTCCAGGAAGTCCAGCACG2729850.2895162075305967No Hit
TTCTTTGTTTACATTTTAGGCTGCTGGTCTGGCTACTATGATCTCTACCA2708530.28725509957794276No Hit
ACGTGAGGAATGTGATTTTCTCAATAAGGCATTAAGAGTGGTCTTAGGAA2625060.2784026286207184No Hit
ATCATCTCCCACTAGAGACAGAATGGGACCATTGTCTGCAGGAACGGTGG2613510.2771776850534974No Hit
TTTTGGACATAGGTCATTAGTAACAAGTAAGTTGTTACAATTGCTGCCAA2552720.2707305578282707No Hit
TGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTTCCTCCTC2539320.2693094111788541No Hit
GAGATGGCTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTG2440120.25878868374436675No Hit
GAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAGATCTGGAGGATCAC2247750.23838674486762962No Hit
AAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCAA2244610.23805372990649767No Hit
GCACTCGCTAAAATTGGAAACATGCATTTTAGCAAAAAGCACAACTGGCA2214850.23489751167615147No Hit
TAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG2169620.2301006114557698No Hit
GCCTGGCTTCTCTCATAATTTTGAATTTAAATAAATAATGCAGATTGACT2138470.22679697577447666No Hit
GGTCTCCTCTCTTTCGTGTCAAAGGACTTCTTTGCCAAGTTCACAGATAA2128660.22575656916023956No Hit
TCTTTTAGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGA1971590.20909839720323428No Hit
GGCAAACCCTGAAGAACTGTAACCAGTTGCATTTACAAAATCATAATAAC1862680.19754786872652044No Hit
TTCCTGGAATGGTAGCCATCTGGTGATGGGACTATGAAGGTCGGGCACAA1848420.1960355141578129No Hit
CACGTAATCAGCAATGAGTATTCTCTTCATTTCAGGTCAGTTGATTTATT1812600.19223659826362607No Hit
CTCCTGTTAAATTATAGTTTACACTGACACCTAGCTGTGATCCTGAAACT1732320.18372244505353896No Hit
CGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT1648280.17480952233585434No Hit
TCGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA1639480.173876231998924No Hit
GAGAACTTGCCGGTTAAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCA1598030.16948022240054195No Hit
CGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC1587860.1684016357270668No Hit
CGTGTGTTTGCGGGGAGTTTGAAGAGAGTTATGACGTGTGTGCGTGATTT1448730.15364610338875812No Hit
TGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT1424410.15106682827578705No Hit
TGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA1418630.15045382621357598No Hit
TAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT1402140.1487049673890327No Hit
GGATCAGTGCATAACAGCCTAATCTCGTCGCCCACGCGGACTATTAAGTC1393590.1477981909821288No Hit
TCCTTGTTTTGGAGATGCAGGGTATGAAGAGAGCTGTTGAACATGCCCGG1371420.14544693566738498No Hit
CCTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTTGTGTTTGT1321850.14018975362174452No Hit
TGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC1280740.1358298029681984No Hit
CATGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTAT1261500.1337892909133644No Hit
TGTGACTGGCCCTGACATTTTCCACTTCTTGTTCCGACTACTGCGAGTAC1225810.13000416226279127No Hit
GCTCTTTATCTAAATAGAGCCATTCCTTTATGTTTTAGGCAAGATAAACA1158710.12288782344369753No Hit
CATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG1157820.12279343385280343No Hit
TCCATTAGGCATTAGCACTGCCTTGTTTTTAAGTAATACAATGTTCTTGT1120220.1188057387768284No Hit
CACGACAGCGATGAGAGTGACACTGCCAAGGCCGTGGAGGTGCAGAACAA1101090.116776892851206No Hit
CCCAGTGAAAATTAAGTGCTATGGTAAGATAACCTCTTTTGTTGCTGGTG1097840.11643221176086242No Hit
TGGTTGTCCTTTTATTCTTAAGAGTCAATTTTATTTTTTCTGTTTTAGTG1058390.11224831360269182No Hit
TGCCCAGAGAGAGAAGGTTCACTAACTGTGCGTTTTATTCCTCCATTTTG1048820.11123336036128009No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG998370.10588284928194658No Hit
CCTTCATGTCTTAGCACCTCACCTAACTCTGGCTTGAATAACCTTCCCAT990370.10503440352110081No Hit
TAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG985070.1044723082045405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)