FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97028234
Sequences flagged as poor quality0
Sequence length139-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA19445942.004152729400393No Hit
GCTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTTGTGTTTGT14162741.459651424759519No Hit
GGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT13367921.3777350621469624No Hit
TTTTAAATCCCACCCCACTTTACATATAATTGACCTTTCTGATTCTCTTC13034781.3434007260196037No Hit
CATTCTGGAAAATGTGGAGGCTTGCACTTTCAAGAAAAGTTAAAACTCCA12794431.3186295856935826No Hit
GATGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTAT12660041.3047789780446792No Hit
GATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG12563491.294828266172504No Hit
TGGTCCTTTCGGCAAGAGACTTGATGAAACGCAGGTAAGTGGGCTCTGAA11704851.2063344366341864No Hit
AGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT11554601.1908492532183983No Hit
CAGAAAGGACAAGGGTGGTTGGGAGTAGATGGAGCCTGGTTTTTTAAATG11319141.1665820899100359No Hit
CTTAGCCTCCCCATCCTTGTAGAGGGGAACATCTGAGGCCACAGATTTTG11057121.1395775790374585No Hit
TGTGACTGGCCCTGACATTTCAACTTTTACTTGCTTCTGTGATTTGAGAG11008001.134515135048217No Hit
TATCTGAAGTGTGTGAAGAGTTTGGATAAGGACTAACTGGATTGGGCCGT10872181.1205171476170532No Hit
AAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG10794151.1124751585193233No Hit
TCCTTGTTTTGGAGATGCAGGGCATGAAGAGAGCTGTTGAACATGCCCGG10511491.0833434317685304No Hit
GGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC10077331.0385976931209528No Hit
TCTGTGGGGAGTAGAGTATATGTAGAGTGGTTGTTAGGACTGAAAATGAT10014331.0321047376787256No Hit
CCAAATTTTATGACAAAAGTTGTGGACAGGTTTTGAAAGATATTTGTGTT9831921.0133050551038576No Hit
CGCTGTTTCATGCTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTG9826001.012694923417858No Hit
TTGGTCCACTGTACCTTGGGACTGCCCTTGATTCATTTCAACTTGGTACA9734891.0033048730949798No Hit
GCCAGTGAAAATTAAGTGCTATGGTAAGATAACCTCTTTTGTTGCTGGTG9055010.9332345469670199No Hit
TCTGCATTATAAAAAGGACAGCCAGATATCAACTGTTACAGAAATGAAAT8621210.8885259109219693No Hit
AGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC8531540.8792842710091993No Hit
TTGGGTCTTGTTTCCTGCAAAAAGTTCAGGATGTGTAGTCTGTTCTTTAT8527980.878917367495321No Hit
GACTCCTCTTTGCATCGGGTAATTATTTATCACTGTATCTGGTCCCCTCC8134650.8383796823510155No Hit
GCTTCATGTCTTAGCACCTCACCTAACTCTGGCTTGAATAACCTTCCCAT7878210.8119502618176067No Hit
CTTGAGTGTCAGGTGGCCTTGCTAATTCCTGGAGAGGTCAAGGTGACTTT7681640.7916912102100095No Hit
ACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA7638390.7872337447675283No Hit
TCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAAGCATTGG7606660.7839635626059112No Hit
TCTTCACTGATTGTTAGTTTGCTGCGGATTAAAACACAGGTGATCATATT7446250.767431261296583No Hit
TAGGACTCTGGGAAGGTGGTGCCTCAGGTTTACCCTCTATTTTCACTTCC7358950.7584338801837824No Hit
CTCATCCTCAGGTTTTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAG7204160.7424807917250148No Hit
GTGAAAGAATTTTCCCATTCAACCTCTCTTCCCCAGACTGTTTTCGTGAG7150790.7369803309003852No Hit
CTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCCACCTGTCCCA7132900.7351365376803621No Hit
GGTTTTCTCCCCTCTAAATCTGGTATGGTGGAAATGGGTGCAAAGCCTTG7088930.7306048670328268No Hit
TGTCCTCACAGAGTTCAAGCTGAAGAAGATGTGGAAAAGTCCCAATGGAA7068660.7285157843849863No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA7014780.7229627615401101No Hit
TGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCTTT6983890.7197791521177227No Hit
TTTGTTGCGTTTTCTCCAGAAGGTCCAGTATTCACTCTGTGCGCTTTTGT6653570.6857354530434925No Hit
TGCTCCCCACAATAGGACATCGGCAGAAACTAGATGATCAGACCAAGCCC6542400.6742779632575813No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG6542070.6742439525385983No Hit
GTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCTC6534430.6734565528627471No Hit
TTGATTTATCTTCATTAAAGTTAAGGCGACATAAATCTAAATTACTAAAG6369390.6564470708598077No Hit
CCTTAGGCATTTTGGGATCTGTTCAGTCCTAGTAGGAGAGGTTGCCTTTA6327980.6521792409413532No Hit
AGCCCAGAGAGAGAAGGTTCACTAACTGTGCGTTTTATTCCTCCATTTTG6280810.6473177693824665No Hit
GGCTTCACAAAGTATTGGTATTATAGGAGTGAACCACTATGCCCAGCCAG6230310.6421130987502051No Hit
AAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT6222350.6412927189832188No Hit
TTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGTAATGGG6151820.6340237007714683No Hit
GTTAATCTGCCCTGTGCCTTTGCGTTAATTACCTTTCTTCCATGATTTCT6072370.625835362519326No Hit
ACTCCACAGGTAGTAGGGAAGAATGTACTCGTGTTAATTGTGTATGATCG5935160.6116941178172943No Hit
CCTGATGTCTGCATTTGTCCTTTGACTGGTGTTTAGGTGGTGGCCCTAGG5776720.5953648501940167No Hit
AGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA5601020.5772567189051385No Hit
GAACAAACAGGTCTGAGGATTTACAGTGATAGCTTTTGTTTTCATTCTGT5578780.5749646025712475No Hit
TCAAAAGGAGAGCGTATCCAAGAGGCCTAGCAAGAGAATGAGAATCCGCA5530760.5700155276452832No Hit
GAATACTGTTGCTATGGTGATCTTCTCAACTATCTAAGAAGTAAAAGAGA5508390.5677100131493685No Hit
CTATGAATGTGCCGTTATGCAGGCAGCTGTGTAATTGGATGGGTTTTTTT5422960.5589053594441387No Hit
GTTCTGAGATGCTTTGATTCCCATGTGAGATTTAGCCCTCCTTGACTGAG5398490.5563834131001498No Hit
TGTGTTCACAGAGACCTGGCCGCCAGGAACGTGCTTGTCACCCACGGGAA5351240.5515136965184794No Hit
AAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG5227260.5387359724593154No Hit
TAGGCTAGAAATGTATGGTCAGAAAACACTGTGAAAAGCAAAGCTTACCA5205600.5365036325406067No Hit
CAGAGGTGCTAGGAATCAAAATCTCCAAGAGTGACTATTACTGAGCGGAA5181970.5340682589358474No Hit
GGGCTGTTTCACTACCTCATCATTCGATGGGATGGGTATCCAATGCAAAG5168050.5326336249714696No Hit
TTTTGCATACGGTGATGAGTGAAGAACCTCCAAACCTCTCTCTGAAGGTC5030730.5184810433631102No Hit
TTTGACTGCTAAAATGTGTGATATCCCTAGACAGGATTTACATTATGAAA5018880.5172597493632626No Hit
GTCATGTCTTCAGGGCTTAGGCTCTGTGAGGCCAGGTGTGGAGCCTCCCT4972340.5124632073587982No Hit
CATCGTTTTCACCACTCACAGTGCTGGCTTCATTAGACCCACAGCTCTCC4924080.5074893973644826No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC4877750.5027144985448256No Hit
TCAGTGCTTCAGAGTCTCCGTTGATTTTCAGCTTTTCAATGTCCACCTGT4869540.5018683530816401No Hit
CTAGACCTTCTCTGTCCATTTAGGTGTGAAACCATTCCAGTGTAAAACTT4501190.4639051763015701No Hit
TTAACCCTGATTACTGGTTTCCAACAGGTTCTTGCTGGTGTGAAATGACT4454580.4591014199021699No Hit
CAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCTTTCCTAG4442330.4578389007884035No Hit
GGCAGAAATGGGCTTGAATAGTTAGATGCTTATTTAACCTTGGCAATAGC4245300.43753244029979976No Hit
AGCAGGAACAATGTCTTTTCAAGTCCTTTGCCCATTTTTAAATTGAATTT4224760.43541553070006406No Hit
GTGACGGACTTCAGAGAAGCCTGCTGCCGTCAGTATGAGATGGGGTGAGT4219860.43491052305455746No Hit
GGTCCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCA4135890.4262563410151317No Hit
GTTAATCACAAGGAAGCAGGACACCAATGAAACTTCAAGAAGATGCTCTG4098000.42235129209916367No Hit
GCTGCTGGAACCTGAACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTC4096410.42218742227133593No Hit
AAAAGGTGACATGGAAAGCCCCTGTTTCATACTGACCAAAACTCAGCCTG4080220.4205188357854684No Hit
CGTCAAATGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCA4007670.4130416307484273No Hit
CTGCCAGTAATTTAAACATTCCCATAGAGGTATTTACCTACTACTTCCAA3918100.40381029711413685No Hit
TGACTCAGTTTGGGGTTGCTGTGTTTGCTGCTGTTCTTGCTTCTGTAAGA3900600.40200669838018493No Hit
AAGGTAACATCTTGCCAGACCCATTTTTCAACTGGAGATTTACCTGACAG3857440.3975585085883352No Hit
AATAGGCGAATTGATCTAAAACAGATACATTCAAATTTTACAGGATCTGA3819280.3936256327204719No Hit
CAGCAGACTTTGTGGTCACTAAAATGTTACTGACCTTTCAGAGGTAGGAT3817340.39342569091796514No Hit
AAATCATGACTGATATGGTAGACAGAGCCTAAACATCCCCTTAAATTGGA3734050.38484159157220155No Hit
TCAATTGTGTTACGAAAGGACTGACGATCTTCAAGCCGGGGCTTCTTTTC3708630.38222173558265526No Hit
TTCAACAGATATGTTCAGGCCACCAACCTCATTCTGTTTTGTTCTCTATC3585310.3695120329614574No Hit
TCTATGGCAGTGGTGACCTCTCGCCCCTCGGACCTGCAGAGCACGGGCTT3562580.3671694158630157No Hit
CAGAACTGCCTATTCCTAACTGACTCATCATTTCATCTCTGAAGCAATTT3385200.34888813909567806No Hit
TCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCTTA3366650.34697632443768894No Hit
GGCTTCCCAATAACAGTAGCAGGCCCAGCACCCCCACCATTAATGTGCTG3302920.34040813316255963No Hit
CTTAAAGCCTCCCTTCCTCTTACTCTCTGCCTGCAGGATGTGCGACGTGT3135470.3231502698482588No Hit
CAGTGTGAGAACAGACTCAACAGCTGCTAAGCTGTCCTCAGCCCAACTTA3051750.31452185350503237No Hit
TTCTTTGTTTACATTTTAGGCTGCTGGTCTGGCTACTATGATCTCTACCA3030870.3123699025584656No Hit
ACCATTAGGCATTAGCACTGCCTTGTTTTTAAGTAATACAATGTTCTTGT2973400.3064468843161672No Hit
CTTAAAGCCTCCCTTCCTCTTACTCTCTGCCTGCAGGATGTGCGGCGTGT2848130.29353620926461466No Hit
CAGTGTGTCTCTTTGCAGTTATGATGGTTAACGGTGATCACAGGATAGGT2829860.2916532521863688No Hit
AGGTTGTCCTTTTATTCTTAAGAGTCAATTTTATTTTTTCTGTTTTAGTG2826000.2912554298370513No Hit
CAGCTGTATAGGTACTTGAAGTGCAGTTTCTACTAAATGCATGTTGCTTT2776430.28614660759465127No Hit
TGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTTCCTCCTC2735770.28195607476479473No Hit
GAGATGGCTCCAAGTAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTG2707580.27905073486136006No Hit
GCCTCGTGACCACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGG2684710.27669368897304675No Hit
CAGCAGTAGGGAATCTGGGATCTTTTGGCGGACCGCACATACTCGAGACA2677620.27596297382883417No Hit
TTGACCCATGAGTTGGAGCCACGGCCACTCCCCCAATGTCAGGATCCAGA2661770.27432942868979765No Hit
GAGACGATCCTGAAGAAAGAGAAGAAAAGCAGAAAGATCTGGAGGATCAC2564880.2643436754707913No Hit
TAGGAGTCTGGAATAGAAAGGCTAACAGAAAAGTGTTTGGACAGTGGGTG2235460.23039273290287854No Hit
GGTCTCCTTCCAGGTGCTTTTGCGTGGAGTGTGTGGACCTCTTGGTGGGG2203190.22706689683747103No Hit
CACCCTGGACTCTTTTCTGGCTGCCGCTCTGGCACACCCCTTTTTTGTGG2202280.22697310970330553No Hit
ACGTGAGGAATGTGATTTTCTCAATAAGGCATTAAGAGTGGTCTTAGGAA2194400.22616097495910314No Hit
CCAGTTGCTTTATCTGTTCACTTGTGCCCTGACTTTCAACTCTGTCTCCT2092320.21564032588699905No Hit
TCTTTTAGGTGGAAGATGAAACTGTTTTACATAACATTCCTTATATGGGA1810650.18661063129315536No Hit
CGTGTGTTTGCGGGGAGTTTGAAGAGAGTTATGACGTGTGTGCGTGATTT1785560.18402478602259215No Hit
TTTTGGACATAGGTCATTAGTAACAAGTAAGTTGTTACAATTGCTGCCAA1747890.18014241091927943No Hit
TCGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCTCCA1744560.17979921184590458No Hit
AAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCAA1731560.17845939564354021No Hit
CGGGCACAAGATCTCGTGTTTGCTCCTTGTCTCGACCCTTCAGAATCTCT1687450.17391329620613316No Hit
CCTGTTGCTGGTTTTGAGGGAGATGTGAACTCTGGGATGGTTGTGTTTGT1644100.16944552448517203No Hit
CATATTGTGTAACTTAGGTAATGTTGGGGCATAGTTAAAACCTGTGTTTG1621160.16708126420192293No Hit
GGTCTCCTCTCTTTCGTGTCAAAGGACTTCTTTGCCAAGTTCACAGATAA1611240.16605888137673414No Hit
CATGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTAT1596480.16453767467312658No Hit
CCATGATGCTCTCCTGCTTATGGTGCACCTCCTCCAGGAAGTCCAGCACG1590210.16389147101244778No Hit
CACGTAATCAGCAATGAGTATTCTCTTCATTTCAGGTCAGTTGATTTATT1573640.1621837206683572No Hit
ATCATCTCCCACTAGAGACAGAATGGGACCATTGTCTGCAGGAACGGTGG1549860.1597328876458784No Hit
TAGAGTATGTTTAGTTCCAATCGTCAGAAAATTTTGGAAAGAACGGAAAT1510770.1557041633881536No Hit
CTCCTGTTAAATTATAGTTTACACTGACACCTAGCTGTGATCCTGAAACT1504240.15503116340342749No Hit
TGGTGAGTTTGTATTAAAAGGTACTGGTGGAGTATTTGATAGTGTATTAA1404090.1447094255059821No Hit
TGAACCCTGCATCTAAAGTGGCCCCATGGAGGGAGGCAAAAACCAACTAT1399600.14424667360224241No Hit
TGTCCAAGAATCACTGCACCAAAAGAGAGTTCATATCACTTACCTTGAGC1396420.14391893394658714No Hit
GCACTCGCTAAAATTGGAAACATGCATTTTAGCAAAAAGCACAACTGGCA1350320.13916773956743353No Hit
CGTTGTGGTGAAACATTGGAAGAGAGAATGTGAAGCAGCCATTCTTTTCC1329380.13700960485377894No Hit
TCCTTGTTTTGGAGATGCAGGGTATGAAGAGAGCTGTTGAACATGCCCGG1297750.1337497289706417No Hit
GCCTGGCTTCTCTCATAATTTTGAATTTAAATAAATAATGCAGATTGACT1263550.13022498173057545No Hit
GGCAAACCCTGAAGAACTGTAACCAGTTGCATTTACAAAATCATAATAAC1236610.12744847030813733No Hit
CCCAGTGAAAATTAAGTGCTATGGTAAGATAACCTCTTTTGTTGCTGGTG1120640.1154962791551993No Hit
GAGAACTTGCCGGTTAAGCAGGCCCCCGTGTCTCTCCCTGTTCCCCTGCA1103260.11370504795542295No Hit
CACTCCTCTTTGCATCGGGTAATTATTTATCACTGTATCTGGTCCCCTCC1079820.11128925627977523No Hit
TTCCTGGAATGGTAGCCATCTGGTGATGGGACTATGAAGGTCGGGCACAA1063120.10956810777366102No Hit
GGATCAGTGCATAACAGCCTAATCTCGTCGCCCACGCGGACTATTAAGTC1050650.10828291484723922No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1002200.10328952292381205No Hit
CCTTCATGTCTTAGCACCTCACCTAACTCTGGCTTGAATAACCTTCCCAT993310.10237329476696443No Hit
CTGAAAGAATTTTCCCATTCAACCTCTCTTCCCCAGACTGTTTTCGTGAG992430.10228259951634285No Hit
TAAGTCTGTGTCATGTTTCTGTGAGGTTAAAAGTAATTTGATAGTGTATG971390.10011415852420853No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)