FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83700974
Sequences flagged as poor quality0
Sequence length141-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACTTCCAGTACGTACGAGTCCATGACGTTGTACTCGCAGTAGTCCG5842580.6980301089447298No Hit
AGAAGCTGCGTCAGTACGTACGAGTCTCGACGTTGGTACTCGCAGTAGTC4447260.5313271503865654No Hit
CTGTCACTTTCAGTACGTACGAGTCCGTACATCTGTACTCGCAGTAGTCC3888100.46452267090703153No Hit
TACAAGAGGCAGTACGTACGAGTCATCCTGCCTGTACTCGCAGTAGTCCG3520920.42065460313520364No Hit
CTACGGTATAGTACGTACGAGTCTCAGTGTTCGTACTCGCAGTAGTCCGA3468660.41441094819278923No Hit
CTATTCCGTGTCAGTACGTACGAGTCAGTGTCTCTGTACTCGCAGTAGTC3396060.40573721400183466No Hit
CCAATATACCAGTACGTACGAGTCTGCAGATGCGTACTCGCAGTAGTCCG3233350.38629777474274074No Hit
TCAAGGTCGGTCAGTACGTACGAGTCACATGGATTGTACTCGCAGTAGTC3178180.3797064535951517No Hit
TTCCAATTCTCAGTACGTACGAGTCGGATAATACGTACTCGCAGTAGTCC3048670.36423351537103976No Hit
GGTAGCATCTCAGTACGTACGAGTCGCTCTCCAAGTACTCGCAGTAGTCC2942190.35151203855763974No Hit
TGTGTCTACGTCAGTACGTACGAGTCCGTACATCTGTACTCGCAGTAGTC2744740.32792210996254356No Hit
CCGGTTCTAAGTACGTACGAGTCGTCAGCGTCGTACTCGCAGTAGTCCGA2549350.30457829558829264No Hit
GCGAACTCGTCAGTACGTACGAGTCTATCTGTCAGTACTCGCAGTAGTCC2540410.30351020765899334No Hit
ATCCATTAGCAGTACGTACGAGTCCGCGAGTGTGTACTCGCAGTAGTCCG2412070.2881770527544877No Hit
ATAGTGGTTTCAGTACGTACGAGTCGGATTCGTCGTACTCGCAGTAGTCC2336310.27912578412767336No Hit
GCTCTGTGTCAGTACGTACGAGTCAGGAGGTGAGTACTCGCAGTAGTCCG2308360.2757865159370786No Hit
ACGTATGTCTCAGTACGTACGAGTCCAACCTGGTGTACTCGCAGTAGTCC2258640.26984632221842486No Hit
ACCATTCACTCAGTACGTACGAGTCAGCCACTTCGTACTCGCAGTAGTCC2146100.25640083949321785No Hit
CACATTCTGTCAGTACGTACGAGTCCCATATCTTGTACTCGCAGTAGTCC2029330.24244998630481884No Hit
CTCGAACTGTCAGTACGTACGAGTCTCAATGGACGTACTCGCAGTAGTCC1912250.2284620965103704No Hit
GTAGCCTTGGTCAGTACGTACGAGTCTATTGCTACGTACTCGCAGTAGTC1882510.22490897178807026No Hit
TGCCGAACTTCAGTACGTACGAGTCCCTTGCTTGGTACTCGCAGTAGTCC1686240.20146002124180776No Hit
AGAAGTCCTCAGTACGTACGAGTCGATTATGCGGTACTCGCAGTAGTCCG1683270.20110518666126873No Hit
AGCTTGGTGAGTACGTACGAGTCACGCACACTGTACTCGCAGTAGTCCGA1664640.1988794061106147No Hit
CGTATAGCGAGTACGTACGAGTCTACTGATGAGTACTCGCAGTAGTCCGA1615440.19300133831178595No Hit
CCAACAACATCAGTACGTACGAGTCATAGTGGTTGTACTCGCAGTAGTCC1561970.18661312113285564No Hit
ATCGGTGGTGTCAGTACGTACGAGTCGAGTCTTCGGTACTCGCAGTAGTC1558880.18624394980158773No Hit
ACCGTCACGTCAGTACGTACGAGTCGGACTCCTTGTACTCGCAGTAGTCC1472490.17592268400604275No Hit
GTAGCTACAGTCAGTACGTACGAGTCTTACGGACCGTACTCGCAGTAGTC1467190.17528947751551852No Hit
TACCTCACTGTCAGTACGTACGAGTCCCACTTCGCGTACTCGCAGTAGTC1391740.16627524549475373No Hit
TTACTTACGAGTACGTACGAGTCACGACGATTGTACTCGCAGTAGTCCGA1389660.16602674181545365No Hit
CCTAGCAATTCAGTACGTACGAGTCGTGGTTACCGTACTCGCAGTAGTCC1383560.1652979569867371No Hit
ACTCTCACAGTCAGTACGTACGAGTCTGCGTAATCGTACTCGCAGTAGTC1333130.15927293749293767No Hit
AAGCCACACTCAGTACGTACGAGTCGGTGAGCGTGTACTCGCAGTAGTCC1305090.15592291673929623No Hit
ACGTCGCTCTCAGTACGTACGAGTCGTAGGACGCGTACTCGCAGTAGTCC1279650.15288352558477994No Hit
AGGATGCGTGTCAGTACGTACGAGTCATTGATTGGGTACTCGCAGTAGTC1261340.15069597636940282No Hit
TGACCTATGCAGTACGTACGAGTCCCTATTGGTGTACTCGCAGTAGTCCG1260590.1506063716773475No Hit
GCAACTCGCTCAGTACGTACGAGTCCCGGTGGTTGTACTCGCAGTAGTCC1211390.1447283038785188No Hit
CTAGACATGTCAGTACGTACGAGTCAGTTAGGTCGTACTCGCAGTAGTCC1198890.14323489234426354No Hit
CCGCAGCTATCAGTACGTACGAGTCCTAACACTGGTACTCGCAGTAGTCC1156650.13818835608770813No Hit
GTCGAGTACCAGTACGTACGAGTCATGGTTCGAGTACTCGCAGTAGTCCG1155880.13809636193719801No Hit
AGCATTCCAAGTACGTACGAGTCACGGTGCCTGTACTCGCAGTAGTCCGA1152750.13772241168902047No Hit
TAACACGAGCAGTACGTACGAGTCCGTATTCCTGTACTCGCAGTAGTCCG1139690.13616209531803058No Hit
AACGTCGAGTCAGTACGTACGAGTCACATCGTCCGTACTCGCAGTAGTCC1132970.13535923727721497No Hit
AGCCGGATCGTCAGTACGTACGAGTCGATTGGAAGGTACTCGCAGTAGTC1119110.1337033425680327No Hit
TCGTTCTCCGTCAGTACGTACGAGTCCTGGTCGGTGTACTCGCAGTAGTC1112830.13295305261322288No Hit
TTCATCCAATCAGTACGTACGAGTCAGCCGTTCTGTACTCGCAGTAGTCC1089160.13012512853195712No Hit
AAGCATTGCGTCAGTACGTACGAGTCTGAGTGACTGTACTCGCAGTAGTC1066170.12737844603815482No Hit
ATAACGACAAGTACGTACGAGTCTAGTCAGACGTACTCGCAGTAGTCCGA1062690.12696268026701818No Hit
AACACACTCTCAGTACGTACGAGTCTGGATTAGCGTACTCGCAGTAGTCC1060320.12667952944012337No Hit
CCTTGCTTGTCAGTACGTACGAGTCTCCGGAGCTGTACTCGCAGTAGTCC1037270.12392567857095667No Hit
CCTCTCTAGCAGTACGTACGAGTCTGCGTATCTGTACTCGCAGTAGTCCG1034740.12362341207642338No Hit
CAAGGTGTGCAGTACGTACGAGTCCGAAGAGGTGTACTCGCAGTAGTCCG1033890.12352186009209404No Hit
ACCATTCACTCAGTACGTACGAGTCACACCGCCAGTACTCGCAGTAGTCC1022010.12210252176993783No Hit
TCACAGGTAGTCAGTACGTACGAGTCCTGACAGCAGTACTCGCAGTAGTC997500.1191742404335701No Hit
CGCTGTAGTAGTACGTACGAGTCTCGTAATAGGTACTCGCAGTAGTCCGA989810.11825549365769626No Hit
TACCTCACTGTCAGTACGTACGAGTCCCACTTCGTGTACTCGCAGTAGTC987550.11798548485230291No Hit
GTGTCTGGAAGTACGTACGAGTCAGTACTCTCGTACTCGCAGTAGTCCGA962810.11502972474370489No Hit
TCCAACGTGTCAGTACGTACGAGTCTTACGGACCGTACTCGCAGTAGTCC952750.11382782714093626No Hit
GATACCGGATCAGTACGTACGAGTCTACCTCCTCGTACTCGCAGTAGTCC952710.11382304822402664No Hit
CGGTGAGTCTCAGTACGTACGAGTCACTTAGCTTGTACTCGCAGTAGTCC949120.11339414043138851No Hit
AGGTCACGTGTCAGTACGTACGAGTCTGTATGTAGGTACTCGCAGTAGTC942330.11258291928598106No Hit
GTCGAGTACCAGTACGTACGAGTCCAAGAACCGGTACTCGCAGTAGTCCG939710.11226990022840118No Hit
AGGAGCAATTCAGTACGTACGAGTCTCGCCAAGCGTACTCGCAGTAGTCC935350.11174899828525293No Hit
CAGCTCTTCCAGTACGTACGAGTCACCATGTCTGTACTCGCAGTAGTCCG929460.11104530277031184No Hit
GCGGAGATTGTCAGTACGTACGAGTCAGCACCAGCGTACTCGCAGTAGTC922040.11015881368357791No Hit
CAAGCCTCGTCAGTACGTACGAGTCTGATACCGTGTACTCGCAGTAGTCC920650.10999274632096873No Hit
GGATGCGAATCAGTACGTACGAGTCACACGTTGCGTACTCGCAGTAGTCC891240.10647904766317294No Hit
CTATCCATCGTCAGTACGTACGAGTCCGACCACTTGTACTCGCAGTAGTC878760.1049880255873725No Hit
GATTAGACATCAGTACGTACGAGTCTCGAACGCTGTACTCGCAGTAGTCC872310.10421742523569678No Hit
AATGCACTTCAGTACGTACGAGTCCGGAGACTTGTACTCGCAGTAGTCCG869660.10390082199043466No Hit
TACACGGTCCAGTACGTACGAGTCACCATGTCTGTACTCGCAGTAGTCCG868900.10381002256915195No Hit
ACCGCTGTGTCAGTACGTACGAGTCCCGATCTCTGTACTCGCAGTAGTCC861630.10294145442082908No Hit
CTCATGCCAGTCAGTACGTACGAGTCATCCGTCTCGTACTCGCAGTAGTC860950.10286021283336559No Hit
TTCGTGATGGTCAGTACGTACGAGTCTTCCTCGGTGTACTCGCAGTAGTC856350.10231063738875966No Hit
CTCGAACTGTCAGTACGTACGAGTCTCGTCATTCGTACTCGCAGTAGTCC844750.10092475148497078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)