FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80608381
Sequences flagged as poor quality0
Sequence length141-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACAGAAGTACGTACGAGTCACTACCATCGTACTCGCAGTAGTCCGA2254894027.973443605076255No Hit
GTATACAGAAGTACGTACGAGTCACTACCATTGTACTCGCAGTAGTCCGA878011610.892311557528988No Hit
ACCGATCGATCAGTACGTACGAGTCGCCTTATCTGTACTCGCAGTAGTCC42777625.306845202609887No Hit
GTATACAGAAGTACGACGAGTCACTACCATCGTACTCGCAGTAGTCCGAC20369832.5270114282533473No Hit
GTATACAGAAGTACGACGAGTCACTACCATTGTACTCGCAGTAGTCCGAC11203551.3898740876584532No Hit
CTTCCGTGGGTCAGTACGTACGAGTCAGCCGTTCTGTACTCGCAGTAGTC9038551.1212915937363883No Hit
GTATACAGAAGTACGTACGAGTCACTACCATAGTACTCGCAGTAGTCCGA6346820.7873647778634829No Hit
ATATTCGCACAGTACGTACGAGTCTACGGTCGTGTACTCGCAGTAGTCCG4662920.5784659041843304No Hit
TGCCAATGGTCAGTACGTACGAGTCCTAACCGGTGTACTCGCAGTAGTCC4478180.555547691746842No Hit
ATGAGTGTGTCAGTACGTACGAGTCCGTATTCCTGTACTCGCAGTAGTCC3578170.4438955299201457No Hit
AATCCGCTGGTCAGTACGTACGAGTCCGAACAGAGGTACTCGCAGTAGTC3303250.4097898951723146No Hit
CCACTTCGCGTCAGTACGTACGAGTCAGATCGAGTGTACTCGCAGTAGTC3227660.40041245835219047No Hit
CTGGTGACATCAGTACGTACGAGTCGACTTGATTGTACTCGCAGTAGTCC2815730.34930983169107443No Hit
AGCCTCGCAGTCAGTACGTACGAGTCAAGGATCGTGTACTCGCAGTAGTC2566030.31833290387013236No Hit
GTATACACATTGTACTCGCAGTAGTCCGACTGAGATACTAGATCGGCTCT2410960.29909545013687844No Hit
ACTCCAACTGTCAGTACGTACGAGTCTGGATAGGTGTACTCGCAGTAGTC2378240.2950363188661487No Hit
GTATACACATCGTACTCGCAGTAGTCCGACTGAGATACTAGATCGGCTCT2246510.2786943456909276No Hit
ATGCGCCGTGTCAGTACGTACGAGTCCTCCGCATTGTACTCGCAGTAGTC2230100.2766585772266038No Hit
AATCCGCTGGTCAGTACGTACGAGTCCGAACAGAGGTACTCGCAGTAGTG2047950.25406167132918844No Hit
TCACTACCATTGTACTCGCAGTAGTCCGACTGAGATACTAGATCGGCTCT1890610.2345426091611988No Hit
AATACTCCGTCAGTACGTACGAGTCTGTCAACCTGTACTCGCAGTAGTCC1637610.20315629462896667No Hit
TCACTACCATCGTACTCGCAGTAGTCCGACTGAGATACTAGATCGGCTCT1637450.20313644557629806No Hit
CCTCATAACAGTACGTACGAGTCGCGTGAGCTGTACTCGCAGTAGTCCGA1634430.20276179470717814No Hit
ACCGACGGTTCAGTACGTACGAGTCCACTTCCTTGTACTCGCAGTAGTCC1631880.20244545043027226No Hit
CACAACGCTGTCAGTACGTACGAGTCCTCATTAGTGTACTCGCAGTAGTC1347540.16717120270657712No Hit
CCTCAGGTGCAGTACGTACGAGTCGGATCAGGTGTACTCGCAGTAGTCCG1334410.1655423398219597No Hit
ATATTCGCACAGTACGTACGAGTCTACGGTCGTGTACTCGCAGTAGTCCA1322410.1640536608718143No Hit
GTATACAGAAGTACGTGCGAGTCACTACCATTGTACTCGCAGTAGTCCGA1313950.16300414221196183No Hit
GTATACAGAAGTACGTGCGAGTCACTACCATCGTACTCGCAGTAGTCCGA1282110.15905418073090935No Hit
GGAGACACACAGTACGTACGAGTCAGGAGCAATGTACTCGCAGTAGTCCG1280040.1587973836120093No Hit
TCAGTGCAAAGTACGTACGAGTCCTGACGGATGTACTCGCAGTAGTCCGA1212190.15038014471472885No Hit
GTATACAGAAGTACGTACTCGCAGTAGTCCGACTGAGATACTAGATCGGC1197520.1485602346981761No Hit
GTATACAGAAGTACGTACGAGTCACTACCATCGTACTCGTAGTAGTCCGA1148980.142538528344838No Hit
TCCAGCTATGTCAGTACGTACGAGTCGCACATGTTGTACTCGCAGTAGTC1102550.1367785813735671No Hit
GTATACAGAAGTACGAGTCACTACCATCGTACTCGCAGTAGTCCGACTGA1045270.1296726205182064No Hit
GTATACAGAAGTATGTACTCGCAGTAGTCCGACTGAGATACTAGATCGGC1024700.12712077668449884No Hit
GTATACAGAAGTACGAGTCACTACCATTGTACTCGCAGTAGTCCGACTGA1019130.12642978153847304No Hit
GTACGAGTCACTACCATTGTACTCGCAGTAGTCCGACTGAGATACTAGAT995940.12355290946731705No Hit
TAGAGACTAAGTACGTACGAGTCCAGCGACGTGTACTCGCAGTAGTCCAA951630.11805596244390518No Hit
GTGTCTGGAAGTACGTACGAGTCCGGTTCTATGTACTCGCAGTAGTCCGA939320.11652882595421436No Hit
GTATACAGAAGTACGTACGAGTCACTACCATGTACTCGCAGTAGTCCGAC919990.1141308122786885No Hit
CCTAGAGTAGTCAGTACGTACGAGTCACGCACACTGTACTCGCAGTAGTC893690.11086812424628649No Hit
TGCGCTGCAGTCAGTACGTACGAGTCAGTGTCTCTGTACTCGCAGTAGTC876870.10878149258449936No Hit
CGCAGTGTCAGTACGTACGAGTCGCCAACAATGTACTCGCAGTAGTCCGA862530.10700252123907562No Hit
GCGTCGTCTCAGTACGTACGAGTCTAGTCGCATGTACTCGCAGTAGTCCG853390.10586864410538155No Hit
GTACGAGTCACTACCATCGTACTCGCAGTAGTCCGACTGAGATACTAGAT852840.10580041298683322No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)