FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007271924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007271924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80608381
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATGACTACGCTACTCATGGCAGCCAGAAGAGCCGATCTAGTATCTC4331498453.73508742223715No Hit
CGAGATGACTACGCTACTCATGGGCAATGAGTGAGCCGATCTAGTATCTC68262138.468366335257373No Hit
CGAGATGACTACGCTACTCATGGAACCGAACAGAGCCGATCTAGTATCTC30980213.8432988748403223No Hit
CGAGATGACTACGCTACTCATGGAACACCGTTGAGCCGATCTAGTATCTC23614432.9295254050568262No Hit
CGAGATGACTACGCTACTCATGGAACGCCTCAGAGCCGATCTAGTATCTC22271622.7629409899697652No Hit
CGAGATGACTACGCTACTCATGGCCAACACTAGAGCCGATCTAGTATCTC18945242.350281666121045No Hit
CGAGATGACTACGCTACTCATGGGACTGACAAGAGCCGATCTAGTATCTC18834592.3365548056349126No Hit
CGAGATGACTACGCTACTCATGGTCGTGCCAAGAGCCGATCTAGTATCTC15535791.9273169622399438No Hit
CGAGATGACTACGCTACTCATGGGCCTGTTGAGAGCCGATCTAGTATCTC14162131.756905401685217No Hit
CGAGATGACTACGCTACTCATGGCCTTAAGGTGAGCCGATCTAGTATCTC14144471.7547145624969194No Hit
CGAGATGACTACGCTACTCATGGCCTGGAATCGAGCCGATCTAGTATCTC13571121.6835867228247643No Hit
CGAGATGACTACGCTACTCATGGCAGTAACCTGAGCCGATCTAGTATCTC13285331.6481325930612598No Hit
CGAGATGACTACGCTACTCATGGCCTCTATGCGAGCCGATCTAGTATCTC7142180.8860344186791198No Hit
CGAGATGACTACGCTACTCATGGCCTTAGACCGAGCCGATCTAGTATCTC6695530.8306245475889164No Hit
CGAGATGACTACGCTACTCATGGAACGGACGTGAGCCGATCTAGTATCTC4665000.5787239418690223No Hit
CGAGATGACTACGCTACTCATGACAGCCAGAAGAGCCGATCTAGTATCTC1763330.21875268776332327No Hit
CGAGATGACTACGCTACTCATGGCAGCCAGAAGAGCCGATCTAGTATCTA1139560.14136991536897384No Hit
CGAGATGACTACGCTACTCATGGCAGCCAGAAGAGCCGATCTAGTATTCA1101490.13664708139963758No Hit
CGAGATGACTACGCTACTCATTGCAGCCAGAAGAGCCGATCTAGTATCTC1077480.13366848293355502No Hit
CGAGATGACTACGCTACTCATGGCAGCCAGAAGAGCCGATCTGTATCTCA1075980.13348239806478684No Hit
CGAGATGACTACGCTACTCATGGCAGCAGAAGAGCCGATCTAGTATCTCA1012520.12560976755010128No Hit
CGAGATGACTACGCTACTCATGGCAGCCAGAAGAGCCGATCTAGTTCTCA930810.11547310446540292No Hit
CGAGATGACTACGCTACTCATGGCAGCCGAAGAGCCGATCTAGTATCTCA871630.10813143610960256No Hit
NGAGATGACTACGCTACTCATGGCAGCCAGAAGAGCCGATCTAGTATCTC863340.10710300706821044No Hit
CGAGATGACTACACTACTCATGGCAGCCAGAAGAGCCGATCTAGTATCTC861940.10692932785736015No Hit
CGAGATGACTACGCTACTCATGGCAGCCAGAGAGCCGATCTAGTATCTCA848690.10528557818324127No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)