FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007273151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007273151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56855705
Sequences flagged as poor quality0
Sequence length51
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC19513383.43208830142903No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCC14171002.492449966102786No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCC8794171.5467524323196766No Hit
GGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCA7423331.3056438223745532No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGG6127691.077761677566042No Hit
GGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCG5908251.0391657266408005No Hit
GCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCG5549500.9760673972822955No Hit
GGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCG5388340.9477219568379287No Hit
AGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCC5241070.9218195429992468No Hit
CGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTC4865720.8558015418153727No Hit
GGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGC4657770.8192264962680527No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCC3890090.6842039862138725No Hit
CAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGA3682490.6476905000122679No Hit
GTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGC3177230.5588234285372067No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGT3099040.5450710707043384No Hit
CGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACC3081410.5419702385187204No Hit
GTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCG2822090.49636003985879695No Hit
GGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCC2812570.49468562565533225No Hit
GAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCC2785140.48986113178967705No Hit
CTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG2776180.4882852125393573No Hit
GGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGAC2372040.4172035154607616No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGG2356210.4144192741959668No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2213950.38939803842024295No Hit
CGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGG2202580.38739823910371No Hit
GGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCC1960910.3448923903063026No Hit
CCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGT1843320.32421020898430514No Hit
GGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCA1840150.32365265719596653No Hit
CGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCG1803250.3171625433190917No Hit
AGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCC1766540.31070584737274826No Hit
GTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGGCCCGGCGGGGCGTGCGC1725760.3035333041776547No Hit
AGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACC1706250.3001018103636214No Hit
GGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGAC1695310.29817764110039613No Hit
GGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCC1685150.29639066123619434No Hit
CGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCC1663760.2926285057937458No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG1524450.2681261273604821No Hit
GCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGG1518420.26706554777572455No Hit
GGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCC1505870.26485820552220746No Hit
GCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGC1505480.2647896108226958No Hit
GGGAGCGAGACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCCACCCCCGC1497490.26338429890193077No Hit
GTGCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGT1491170.26227271300215166No Hit
AGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGC1452930.2555469147731085No Hit
CACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAAC1435970.25256392476357475No Hit
CCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGAC1385450.24367827291913802No Hit
GGTTTGTCCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGGCCCGGCGGGGCG1325070.23305840636397No Hit
CTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGT1298600.2284027609894205No Hit
GCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCA1288940.22670372304766953No Hit
CGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAACC1255110.22075357257464312No Hit
GAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCAC1226030.21563887036490006No Hit
GCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGG1209720.21277020485455245No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTC1197900.21069125780781364No Hit
GGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACG1184950.2084135620163359No Hit
GGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAG1181190.20775223875950533No Hit
GTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCG1177220.20705397989524535No Hit
CCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGG1173560.20641024502290492No Hit
GAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGC1167660.20537253033798458No Hit
CCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTG1161980.20437351009894256No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGA1151400.202512659019882No Hit
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCC1144280.2012603660441815No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC1055480.18564188061690554No Hit
CCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCC1045050.18380741211458024No Hit
CGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGCCCCCGGGGAGCCCG1008220.17732961010684856No Hit
CCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGA1006880.1770939257546802No Hit
GGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGA992270.17452426278066555No Hit
GGGCACGGTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGG984510.17315940414422792No Hit
GTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGT963010.16937790147884016No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTC961340.16908417545785423No Hit
CCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGCGGG948750.16686979785054112No Hit
CCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGC939780.16529211976177238No Hit
GGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTG935750.1645833078668183No Hit
GTTTGGGAGCCGCGGAGGCGGCCGCGCCGAGCCGGGCCCGTGGCCCGCCGG933210.16413656290076784No Hit
CCGGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCGCGCGCGGGGCGCG918310.16151589361173166No Hit
CACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGA914350.1608193935859207No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCT906280.15940001095756354No Hit
CGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGA902320.15870351093175258No Hit
GGAAGACGAACGGAAGGACGGACGGCGCCGGACGCGCACGCCCCGCCGGGC899840.15826731899639623No Hit
GCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGT897280.15781705635344773No Hit
GAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCC895600.15752157149401277No Hit
GGCACGGTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGC883650.1554197595474368No Hit
CCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGA882930.15529312317910754No Hit
GCCGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGG881260.1549993971581216No Hit
GGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCG877620.1543591799626792No Hit
GTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGG868510.15275687813562422No Hit
CCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGG838050.1473994562199167No Hit
CTCGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCCGGACCCCGTC835330.14692105216178394No Hit
AGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC832550.14643209507295707No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAG829720.14593434379188508No Hit
GGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCC821080.14441470737193393No Hit
CGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCG820970.14439536014899473No Hit
CCCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGG820070.14423706468858316No Hit
CGGTCCCCCGCGAGGGGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCG819410.14412098135094797No Hit
CGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAGGC808490.1422003297646208No Hit
GGGACGGGGACCGGCGGGCCACGGGCCCGGCTCGGCGCGGCCGCCTCCGCG792500.13938794708464172No Hit
GGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGT789750.13890426651116192No Hit
CGGGACCCCGGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGA788040.1386035051363799No Hit
CGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGG778190.1368710492640976No Hit
GCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGC776280.13653511112033523No Hit
GGGTGGCCGCCCCACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCG765810.13469360726421387No Hit
GCCGCGCCGAGCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGC763260.13424510345971438No Hit
GGAGAGGCCGGGAGGGAGGAAGACGAACGGAAGGACGGACGGCGCCGGACG761520.13393906556958532No Hit
GGGGCCCGGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGC753640.13255310087175948No Hit
ACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAA752500.13235259328857146No Hit
CCGCGCGCGCGCGCGCGCGTGCGTGCGGGGGGCCCGGCGGGGCGTGCGCGT726110.12771101862161413No Hit
CACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGG723840.12731176229368715No Hit
CCCGGCTCGGCGCGGCCGCCTCCGCGGCTCCCAAACCACGCTCCCCGGACC722340.1270479365263345No Hit
GCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCAC720490.12672255141326627No Hit
GGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAG717610.1262160059399492No Hit
GGTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCGCGCGCGGGGCGCGTG716690.1260541928026396No Hit
GGCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC716010.1259345917881064No Hit
GTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCGCGCGCGGGGCGCGTGG708440.12460315108220012No Hit
CGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCG705560.12409660560888304No Hit
CCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGGAGGAG702770.12360588968160714No Hit
CCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCC692470.12179428607911906No Hit
GGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTG690240.12140206510498815No Hit
GCCGGGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCC689430.12125959919061774No Hit
GCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTC675460.1188025018773402No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCG668060.11750096142506719No Hit
CGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCA666380.11720547656563225No Hit
GCGGGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTC661630.11637002830234891No Hit
CGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGGG650890.11448103580810404No Hit
CACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGC646090.11363679335257562No Hit
GGCCCGTGGCCCGCCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGG635150.11171262408935038No Hit
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCGGGTGGCCGCCCCACCGGG634580.1116123702977564No Hit
CCGGTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACC630200.11084199905708671No Hit
GGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGG623790.10971458361126644No Hit
GGGCACCCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCC620690.109169343692071No Hit
CCGGACCCCGTCCCGGCCCGGAGCGGACGAGCCGCCCCGGCGGTGAACGGG615840.10831630704429748No Hit
GTCCTCCTCCTCCCCCGTCTCCGCCCCCCGGCCCCGCGTCCTCCCTCGGGA603210.1060948940831883No Hit
CCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCG599290.10540542941117344No Hit
CGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGG597550.10509939152104437No Hit
CCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAA594280.10452425134821564No Hit
CCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACCGGCGGGCCACGGGCC588000.1034197008022326No Hit
CTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGC580090.1020284595890597No Hit
GCAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT575620.10124225880234887No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGC568700.10002514259562871No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)