FastQCFastQC Report
Thu 3 Nov 2022
EGAF00007273153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007273153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157792
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT10790.6838116000811195No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGG7210.4569306428716285No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCC6970.44172074629892516No Hit
AGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTACGAA6320.40052727641452035No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC6180.3916548367471101No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAAC5530.35046136686270535No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGG5390.34158892719529504No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTT5070.3213090650983573No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAA4920.31180287974041776No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG4800.3041979314540661No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGA4330.2744118839991888No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCC3980.2522307848306632No Hit
GTTACTCCCGCCGTTTACCCGCGCTTCATTGAATTTCTTCACTTTGACATT3940.24969580206854594No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCAT3900.24716081930642875No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA3800.24082336240113567No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCG3720.23575339687690122No Hit
GGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTAC3610.22878219428107888No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTG3540.22434597444737375No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCA3510.22244473737578585No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGG3470.21990975461366863No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGG3450.21864226323261002No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTG3450.21864226323261002No Hit
CAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTG3170.2008973838977895No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAA3160.20026363820726018No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC3130.19836240113567227No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCA3110.19709490975461366No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATT3100.19646116406408434No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAA3100.19646116406408434No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGC3100.19646116406408434No Hit
CATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTC3070.19455992699249644No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCA3050.19329243561143783No Hit
GAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTC3010.1907574528493206No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATT3010.1907574528493206No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGC3000.19012370715879132No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCT3000.19012370715879132No Hit
CACACCCCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGCGC2950.1869549787061448No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCC2900.1837862502534983No Hit
CTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCAC2890.18315250456296897No Hit
AGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGT2780.17618130196714663No Hit
CCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAA2760.17491381058608801No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT2760.17491381058608801No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGCG2740.1736463192050294No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGAC2730.17301257351450008No Hit
GGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATC2720.1723788278239708No Hit
CGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAG2710.1717450821334415No Hit
ATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGA2660.16857635368079496No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCA2650.16794260799026567No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCG2600.16477387953761916No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGC2590.16414013384708984No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG2580.16350638815656054No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGA2580.16350638815656054No Hit
GGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCT2570.16287264246603123No Hit
GGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGG2430.15400020279862098No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAGG2430.15400020279862098No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCA2420.15336645710809166No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTT2390.15146522003650376No Hit
CTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTC2390.15146522003650376No Hit
GTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATC2380.15083147434597444No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCT2370.15019772865544515No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTAC2340.14829649158385724No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCT2330.14766274589332792No Hit
CGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACACC2320.14702900020279863No Hit
GATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATC2320.14702900020279863No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACA2300.14576150882174002No Hit
CCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACGAACGGAAG2300.14576150882174002No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTC2290.1451277631312107No Hit
AGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGA2280.1444940174406814No Hit
CTTGGAGACCTGCTGCGGATATGGGTACGGCCCGGCGCGAGATTTACACCC2280.1444940174406814No Hit
CAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCA2250.1425927803690935No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT2220.14069154329750558No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTG2210.14005779760697626No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAA2190.13879030622591765No Hit
CCACACCCCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGCG2180.13815656053538836No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC2170.13752281484485904No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAG2160.13688906915432975No Hit
CTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGG2160.13688906915432975No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACT2140.13562157777327113No Hit
ATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC2120.13435408639221252No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACC2060.13055161224903672No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGG2040.1292841208679781No Hit
GTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACA2030.1286503751774488No Hit
GTTGAGATCGTTTCGGCCCCAAGACCTCTAATCATTCGCTTTACCGGATAA2030.1286503751774488No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATG2020.1280166294869195No Hit
CTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCA2000.1267491381058609No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACT1980.12548164672480228No Hit
CAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACAT1980.12548164672480228No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCC1950.12358040965321437No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGG1940.12294666396268507No Hit
CCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGAT1940.12294666396268507No Hit
GTTTCCTCTGGCTTCGCCCTGCCCAGGCATAGTTCACCATCTTTCGGGTCC1940.12294666396268507No Hit
CGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCC1940.12294666396268507No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGA1930.12231291827215574No Hit
AGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG1910.12104542689109712No Hit
CTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGT1910.12104542689109712No Hit
GTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGA1900.12041168120056783No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCC1900.12041168120056783No Hit
CAAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTG1900.12041168120056783No Hit
GGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTC1890.11977793551003853No Hit
CTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGT1880.11914418981950922No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAG1860.11787669843845061No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAAC1850.1172429527479213No Hit
CCCACACCCCCGCCGCCGCCGCCGCCGCCCTCCGACGCACACCACACGCGC1850.1172429527479213No Hit
GTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGC1840.116609207057392No Hit
AGAACTCTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACC1840.116609207057392No Hit
GTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAA1800.11407422429527479No Hit
ATTTCACCGGCGGCCCGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCG1800.11407422429527479No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATA1790.11344047860474549No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAG1780.11280673291421618No Hit
CCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCAC1760.11153924153315757No Hit
AAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCG1760.11153924153315757No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTC1730.10963800446156967No Hit
CAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTA1730.10963800446156967No Hit
CAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCA1720.10900425877104036No Hit
CTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAAT1710.10837051308051106No Hit
GGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATT1710.10837051308051106No Hit
CTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACC1700.10773676738998175No Hit
ACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1700.10773676738998175No Hit
CCCGACTCGCGCACGTGTTAGACTCCTTGGTCCGTGTTTCAAGACGGGTCG1690.10710302169945245No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCC1680.10646927600892314No Hit
ATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAG1670.10583553031839385No Hit
CACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG1660.10520178462786454No Hit
CAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATC1640.10393429324680593No Hit
AACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTG1640.10393429324680593No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTC1630.1033005475562766No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGA1630.1033005475562766No Hit
ATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAAC1630.1033005475562766No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGTC1620.10266680186574731No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGAC1620.10266680186574731No Hit
CGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCC1610.102033056175218No Hit
AGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCCGT1590.10076556479415939No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCG1580.10013181910363009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)