FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5772403
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA2129093.6883945906063733No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA1358052.352659715546541No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAACTGTAGGCACCATCAA738581.2795017950063432No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT706531.223978991071829No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA522690.9054981088465237No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT519020.8991402713913079No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA467730.8102864612883058No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAA416620.7217444797253414No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATC375940.6512712296767914No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCC367840.6372389453750891No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA352480.610629576625194No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTA352320.610352395700716No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCA352290.6103004242773763No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAC332700.5763630848365923No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC323590.5605810959491221No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAACTGTAGGCACCATCAAT322650.5589526580178134No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAACTGTAGGCACCATCAAT310250.5374711363707627No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATA308180.5338851081603276No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTT306300.5306282322977103No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAT293800.508973472572861No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA282330.4891030650493391No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTC271250.46990828602923257No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCG268240.46469381988748876No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATT263660.45675951592430397No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT255580.4427618792381613No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGA253660.43943570814442445No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAG253170.4385868415632104No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT252230.43695840363190164No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT251340.43541658473949235No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCAACTGTAGGCACCATCAA231710.4014099500675888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCT228000.3949828173812535No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC216530.3751124098577317No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTG206750.3581697258490095No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGT196710.3407766228380104No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAACTGTAGGCACCATCAA193580.33535427100290816No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGC166380.2882335138416358No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA165200.28618930452360997No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATG163390.2830536953154518No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAACTGTAGGCACCATCAA159890.276990362592494No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG157500.2728499725331028No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA156550.2712042107940142No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA140830.2439711849640436No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT132730.22993890066234113No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAACTGTAGGCACCATCA128790.2231133203970686No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAACTGTAGGCACCATCAA128040.22181403481357762No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTAACTGTAGGCACCATCAA107230.1857631908236483No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATAA104000.18016760091074724No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC102690.177898182091583No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATCC94580.1638485739821007No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGG93870.16261858362972925No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA91710.15887664114927527No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATTA89040.15425118447204741No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATCA87360.15134078476502766No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA85680.1484303850580079No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGAACTGTAGGCACCATCAAT85310.14778940417015235No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATAC84900.14707912805117732No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT81020.14035749063258404No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAACTGTAGGCACCATCAATC78180.13543752922309826No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA78150.1353855577997586No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATTT76160.13193812005156258No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAA75320.1304829201980527No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATAT75060.13003250119577583No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATAACTGTAGGCACCATCAAT72450.12551098736522726No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAACTGTAGGCACCATCAAT72120.12493930170849124No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCAACTGTAGGCACCATCAAT71370.12364001612500028No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATC70180.12157848299919462No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT69160.1198114546056469No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCC68800.11918779752557124No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAACTGTAGGCACCATCAATA68390.11847752140659616No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATTC67960.11773259767206136No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAACTGTAGGCACCATCAA67780.11742076913202353No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATCG67130.11629472162633135No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCA65380.11326305526485243No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATAG63230.10953843659217834No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTA62930.10901872235878196No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGAACTGTAGGCACCATCAATGA62100.10758084631305195No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAC59530.10312862771362291No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC58840.10193328497681121No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAACTGTAGGCACCATCAATT58580.10148286597453436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)