FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5897960
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA5779419.7989982977165No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA4207597.133975137166071No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA2961645.021465048932173No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT1950783.307550407259459No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1698212.879317594558118No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1647212.79284701829107No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA1381382.3421318557602966No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA1219082.0669519630516313No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC972381.6486717441284784No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA879051.49043058955978No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA776181.3160143507246573No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT652281.105941715440593No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC576990.9782874078494936No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT572360.9704372359256421No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC534550.906330324383346No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA439840.7457493777509512No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT419820.7118054378123961No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA378500.6417473160211328No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCAACTGTAGGCACCATCA336790.5710279486466507No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT320130.5427808937327483No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA283220.4801999335363414No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA266240.45141031814390065No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA244420.4144144755135674No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCAACTGTAGGCACCATC228000.38657434095856874No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA223370.37872416903471706No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAACTGTAGGCACCATCAA200600.3401175999837232No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA186690.3165331741822596No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA177660.3012227956785058No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAACTGTAGGCACCATCA172300.29213490766298855No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTAACTGTAGGCACCATCA168540.28575982204016304No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA165620.28080895767350067No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC145890.247356713168621No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA137770.23358924102571058No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATAACTGTAGGCACCATCAAT136330.23114771887228802No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA133040.2255695189523157No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC132620.22485740832423415No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA122140.20708855265210344No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT121390.2058169265305292No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAACTGTAGGCACCATCAA120060.2035619095416042No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA113970.19323630543442138No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTAACTGTAGGCACCAT109150.18506398822643763No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGTAGGCACCATCA104940.17792592693066756No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAACTGTAGGCACCAT101230.17163561638261365No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTAACTGTAGGCACCATCA97540.16537921586446838No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT95740.16232731317269022No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCCAACTGTAGGCACCAT95270.16153042746983703No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCGCAACTGTAGGCACCATCAA94950.1609878669912987No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAATA93620.15873285000237372No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATA92540.1569017083873068No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCAACTGTAGGCACCATCAA91860.15574876737041282No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAACTGTAGGCACCATCAA85630.14518579305386947No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATT79570.13491105399154962No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG78930.13382593303447293No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAATT78000.13224911664372088No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAATC77570.13152005100068498No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATC76230.12924807899680568No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA75450.12792558783036848No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAACTGTAGGCACCATCAA75180.12746780242660175No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT74770.1267726468134745No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC72330.12263562316461962No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC66670.11303908470047269No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCTAACTGTAGGCACCATCA66350.11249652422193436No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT66000.11190309869853306No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG65030.1102584622479637No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAACTGTAGGCACCAT61250.10384946659522953No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)