FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5066078
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA3195956.3085290040935025No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1238482.44465245106767No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT1113622.1981896054502124No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT661541.3058227686190382No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA559381.1041677605437579No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA500980.9888912093339265No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA481780.9509920692101465No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA478840.9451887633786925No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC464410.9167051908794139No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA428010.8448547377280808No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT426800.8424663023348634No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG275490.5437934433698021No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA275290.5433986606601793No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAACTGTAGGCACCATCAA209850.4142257580716286No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA155050.306055295635006No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT148470.29306694448841886No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT148060.2922576399336923No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC147880.29190233549503186No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAAT142390.28106555011588846No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA133650.2638135457053761No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA133140.2628068497958381No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA131310.2591945880027903No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC126760.25021328135887366No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT116710.2303754502003325No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA101860.20106283401084626No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCAACTGTAGGCACCATCAA101570.2004903990818933No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT95240.18799552632233454No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAACTGTAGGCACCAT92220.18203430740703164No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAACTGTAGGCACCATCAA82290.16243334587426408No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA78700.15534699623653642No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG77770.15351125663679083No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA60150.11873089991903006No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAA55350.10925611488808502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)