FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8044537
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA4969616.177620912179284No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA3096423.8490965981012955No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA2697573.3532942915173365No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA1380831.7164816321933754No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT1209891.5039896018875916No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAACTGTAGGCACCATCAA738530.9180515920307161No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA654480.8135707499387473No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA572740.7119614217698297No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC524800.6523681847693659No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC524710.6522563076035327No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT506800.6299927516027337No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAACTGTAGGCACCAT429670.5341140204837146No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCAACTGTAGGCACCATCAA421430.5238710444118785No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA374000.46491177801780265No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA350420.43559996056951444No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT347200.431597244191928No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG325870.40508235588946884No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA279030.34685650647141036No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT272250.3384284266453122No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA259880.3230515317413544No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA244430.3038459516066618No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA239360.2975435379313937No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAA210190.26128290540524585No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATA209510.26043761126339526No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATC203150.25253162487785186No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC195630.2431836661326811No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA194960.24235080278703422No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAACTGTAGGCACCATCAA191980.23864642551833623No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAACTGTAGGCACCATCAAT189440.23548900328260036No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCAACTGTAGGCACCATCAAT186630.231995949549365No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT180190.22399051679419213No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAACTGTAGGCACCATCAA178240.22156651153447363No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATT176090.21889389035068146No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTA175530.2181977657632751No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAACTGTAGGCACCATCAA173100.21517708228577978No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA164260.20418825844172261No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCC161940.20130431372246782No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAC160420.19941483269950774No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAAT153690.19104890685442805No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTT152580.18966908847581904No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCA147640.18352827515119888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAT147580.18345369037397677No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAACTGTAGGCACCATCAAT137960.17149526442603222No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTC128870.16019567067688295No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAG128460.15968600803253188No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGA127330.15828132806151554No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT126450.1571874179955913No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCG120890.1502758953063427No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT120330.14957977071893633No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATG116350.14463231382986988No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTG101810.12655793614971253No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGT101400.12604827350536146No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA101320.125948827135732No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCT90860.11294621430667792No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT90430.1124116900699195No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA87900.10926669863038732No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGC87650.10895592872529518No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCTAACTGTAGGCACCATCA83420.10369770193113662No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTAACTGTAGGCACCATCA82780.1029021309741008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)