FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6512637
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT2662184.087714392802792No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT2631624.040790235967397No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA991051.5217338230274464No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA905531.3904198867524782No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA508320.7805133312358726No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT340640.5230446591756919No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA318390.4888803106944238No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC291330.4473303210358569No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT281920.43288148871186893No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA266960.40991076272176696No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGT240280.36894425407097003No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT238100.36559691565797386No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC237620.36485988701658023No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA233420.3584108864043858No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA219010.336284672399214No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAACTGTAGGCACCATCAA210560.32330989735801335No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAACTGTAGGCACC198460.3047306336895485No Hit
TCCCTGGTGGTCTAGTGGTAGGATTCGGCGCAACTGTAGGCACCATCAAT191630.2942433303130514No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG189830.29147947290782517No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG170470.26175265103828144No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAGG150210.23064390046612454No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG147170.2259760524039648No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT136080.20894761983509905No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA119260.18312090785959667No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA112410.17260289495637482No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT109140.1675818873368806No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCAACTGTAGGCACCATCAA103090.15829225550264817No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC97440.14961681420291043No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCAACTGTAGGCACCATCAA90200.13849996552855626No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT89890.13802396786432286No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAACTGTAGGCACCA71290.109464108010319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)