FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6247581
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA2582144.133023645471743No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA1457192.332406734702599No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA976021.5622366480722698No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT579010.9267746988794543No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA550180.8806288385856863No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA538120.861325367370187No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAACTGTAGGCACCATCAA460720.7374374177781768No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA280330.44870166549261226No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC266590.42670915351077476No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA263640.4219873259746453No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA259540.41542478600917704No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA240150.38438877383102354No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATA219060.3506317084964565No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAA216940.3472383951484582No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA210420.33680235598386No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA201010.3217405264533585No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA191550.3065986659476684No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATC183280.29336154265146785No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATT180660.2891679195515833No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTA180410.2887677646756401No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT168630.2699124669211972No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAC164030.26254961720384257No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCC157850.252657788670527No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTT157570.25220961520947066No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC155520.24892834522673657No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAT149680.23958072732470376No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCA143180.22917670055018097No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA142480.22805626689754002No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAAACTGTAGGCACCATCAAT135970.2176362339279795No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT134210.21481914360133944No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT133550.21376273472884944No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTC133100.21304245595215174No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGA131760.21089762581709623No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATAG130330.2086087399267012No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTTAACTGTAGGCACCATC123180.19716431047472616No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAACTGTAGGCACCATCAA122800.1965560750632925No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAACTGTAGGCACCAT121260.19409112102748247No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGAACTGTAGGCACCATCAATG120460.19281062542446428No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT116840.18701638282080696No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA116030.18571988102275105No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCG114930.18395919956860102No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAACTGTAGGCACCATCAA113550.18175034465339465No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCAACTGTAGGCACCATCAA112770.1805018614404519No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG107560.17216263382579594No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATTG106940.17117024973345685No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGT104200.16678455229311953No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT91590.14660074035054527No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATGC85750.13725312244851245No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAACTGTAGGCACCATCAATCT81930.1311387559441006No Hit
GGTTCCATAGTGTAGTGGTTATCACGTCTGCTTAACTGTAGGCACCATCA79240.12683308947895194No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT73610.1178216016727114No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCAACTGTAGGCACCATCAAT72550.11612494499871229No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTAACTGTAGGCACCATCA72470.11599689543841049No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAACTGTAGGCACC69950.11196333428890318No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)