FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7811574
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA6817168.726999193760438No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA2141362.7412657167428742No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCAACTGTAGGCACCATCA1575462.0168278505714725No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCAACTGTAGGCACCATC1450601.8569881050861197No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA1083971.3876460749139674No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT1076001.3774432655953845No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC995111.273891791846304No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCA991061.268707177324314No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT985351.2613975109241748No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA885761.1339071997525723No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC666300.8529651002474022No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA590000.7552895229565769No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA551800.7063877267244733No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTAACTGTAGGCACCATCAA497740.6371827239939095No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTAACTGTAGGCACCATCA450680.5769387834001188No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT436850.5592342849213231No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAACTGTAGGCACCATCA386020.4941641722910133No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAACTGTAG308670.3951444356796723No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAACTGTAGGCACCATCAA280500.35908256133783023No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAACTGTAGGCACCATCA260930.3340299919068807No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATA252210.3228670687879293No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA250830.3211004593952512No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCAACTGTAGGCACCATCAA236340.3025510607721312No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCCAACTGTAGGCACCAT235250.3011556953822623No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT222760.2851666002267917No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT219410.28087809191847896No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATT217620.2785866203149327No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC215330.2756550728444741No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTAACTGTAGGCACCATCAA209060.2676285214733932No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA193030.24710768918018317No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATC191560.24522586613146083No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA186040.23815942856074845No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTAACTGTAGGCACCATCAA184350.2359959721305847No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTAACTGTAGGCACCAT183620.23506146136489264No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA181700.23260357003594923No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTAACTGTAGGCACCATCA174190.22298963051492568No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGTTTTAACTGTAGGCACCAT169510.21699852040062603No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAATA156740.20065098275968452No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATAACTGTAGGCACCATCAAT147430.18873277011777653No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATG145170.18583962719933267No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG140060.17929805183948844No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA139500.17858116686854658No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAATT136940.17530397842995535No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTTCAACTGTAGGCACCAT136030.17413904035217487No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAACTGTAGGCACCATCAAT135350.17326853717317406No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAACTGTAGGCACCATCAA134930.1727308734449677No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCAACTGTAGGCACCATC134270.1718859733006434No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGCCTTCAACTGTAGGCACCAT127560.1632961551666796No Hit
TCCTCGTTAGTATAGTGGTGAGTATCCCCGCCTGTAACTGTAGGCACCAT122670.1570362131882768No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAATC122670.1570362131882768No Hit
GTTTCCGTAGTGTAGCGGTTATCACATTCGCCTAACTGTAGGCACCATCA107130.13714265524464084No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTAACTGTAGGCACCATC93010.11906691276303598No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTAACTGTAGGCACCATCA90570.1159433425325037No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGGTTTAACTGTAGGCACCATC87320.1117828493975734No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAATG85790.1098242172448216No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAACTGTAGGCACCATC82550.10567652562722954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)