FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007277544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007277544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6144917
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT5486198.928013185532043No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT4208796.849221885340355No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAACTGTAGGCACCATCAA1389102.2605675552655957No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1080831.7589008932097865No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA577480.939768592480582No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA534640.8700524352078312No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT510940.8314839728510572No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC500760.8149174350117342No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA496040.8072362897659968No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAACTGTAGGCACCATCAA457500.7445177860010151No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAACTGTAGGCACCATCAAT452450.7362996115325886No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATT434500.7070884765408548No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATC426430.6939556710041812No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG332620.5412929092451534No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATG287790.46833830302345825No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTAACTGTAGGCACCATCA246600.4013072918641537No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCAACTGTAGGCACCATCAA240850.3919499644991137No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAA144330.23487705366891043No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAACTGTAGGCACCATCAA127640.207716393891081No Hit
AGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAATGTTGGT125920.2049173324879734No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATA120990.19689444137325207No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGAACTGTAGGCACCATCA113920.18538899711745496No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATT104900.17071019836394863No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAACTGTAGGCACCATCA99540.16198754189845038No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATC94450.1537042729787888No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC92390.15035190874018314No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAACTGTAGGCACCATCAAT78530.12779668138723435No Hit
GCCCGGATGATCCTCAGTGGTCTGGGGTGCAGGCTAACTGTAGGCACCAT75000.12205209606574019No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA74540.12130350987653699No Hit
GCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACAACTGT73910.12027827226958478No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAACTGTAGGCACCATCAAT68190.11096976574297099No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGAAACTGTAGGCACCATCAATG65790.10706409866886729No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCAACTGTAGGCACCATCAATA62990.102507487082413No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)